重組蛋白質 的英文怎麼說

中文拼音 [zhòngzǔdànbáizhí]
重組蛋白質 英文
recombinant protein
  • 重 : 重Ⅰ名詞（重量; 分量） weight Ⅱ動詞（重視） lay [place put] stress on; place value upon; attach im...
  • 組 : Ⅰ名詞1 （由不多的人員組成的單位） group 2 （姓氏） a surname Ⅱ動詞（組織） organize; form Ⅲ量詞（...
  • 蛋 : 名詞1. （鳥類或龜、蛇類所產的卵） egg 2. （像蛋形的東西） an egg-shaped thing 3. （辱罵之詞）
  • 白 : Ⅰ形容詞1 （似雪的顏色） white 2 （清楚; 明白; 弄明白） clear 3 （空的; 沒加他物的） pure; clear; ...
  • 質 : Ⅰ名詞1 （性質; 本質） nature; character; essence 2 （質量） quality 3 （物質） matter; substance;...
  • 重組 : bpr
  • 蛋白質 : [生物化學] protein; proteide （舊稱「朊」, 由多種氨基酸結合而成的高分子化合物）
  • 蛋白 : 1. （卵中透明的膠狀物質） egg white; albumen; gary2. [生物化學] （蛋白質） protein
  1. The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss

    將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。
  2. Because, this is of chinese herbal medicine outer the organization is stimulated suddenly by high fever, instantly constrictive, caky even, its protein is formed on cellular wall cannot go against the metamorphic layer that turn, prevent moisture development, organize interior to composition dissolves hard and be released, such, the decoct of medicaments active ingredient goes out rate reduce greatly, affect curative effect badly

    這是因為中草藥的外層組織忽然受到高熱刺激，立即緊縮，甚至凝固，其蛋白質便在細胞壁上形成不可逆轉的變性層，防止水分深入，組織內部成分難以溶解並釋放出來，這樣，藥物有效成分的煎出率大大降低，嚴重影響療效。
  3. The highest antiviral litre of rpoifn - a was l l08iu / mi. the rpoifn - a protected pk - 15 cells against virulent hog cholera virus ( hcv ) infection in vitro

    Sds - page分析表明，構建的重組表達質粒pqe30 poifn在大腸桿菌jm109中表達的rpoifn占菌體蛋白總量的20 26 。
  4. In this paper, the studies on breeding aspect, energy level and feed nutrition, including exogenous porcine somatotropin ( pst ), - incitant, l - novain, lycine, rucca schidigera and electrolyte, which would affect the pork quality were also reviewed

    本文從飼養狀況、飼糧熱能水平、飼糧營養成分（包括蛋白質和氨基酸、脂肪、礦物元素和維生素） 、生長促進劑（包括重組豬生長激素、 -興奮劑、 l -肉堿、甜菜堿、莫哈夫絲蘭提取物和礦物元素）等方面對豬肉品質影響的研究進展做了綜述。
  5. Recovery of this photoinhibition is a complicate but orderly course, including degradation of photodamaged d1, synthesis and assembly of new one, etc. using lincomycin to block the replacement of new synthetic dl protein into photodamaged one, the spinach leaves was exposed to highlight, giving rise to photoinhibition before the thylakiod membranes were isolated

    解除光抑制后， ps活性恢復是一個復雜而有序的過程，需要d1蛋白降解、新合成d1蛋白和重組裝ps等。實驗首先進行菠菜葉片光抑制處理，加入林可黴素阻斷葉綠體蛋白質合成，利用尿素sds變性電泳分離類囊體膜蛋白，藉助d1蛋白抗體westen免疫印跡、磷酸化蛋白快速檢測方法分析d1蛋白存在形式，並進行定量分析。
  6. From the new point of view, looking for tumor - specific proteins with proteomics research strategy may help to elucidate the correlation of the change of protein expression to oncogenesis, and may play a vital role in the early detection of cancer and anticancer therapy including drug exploitation

    腫瘤蛋白質組學從新的角度研究腫瘤，尋找腫瘤標志蛋白，進而闡明其表達水平的變化與腫瘤發生、發展的相互關系，對腫瘤的早期診斷、治療、藥物研發等方面具有重要意義。
  7. This modification includes : ( 1 ) selecting two important molecules as candidates, ( 2 ) choosing a promiscuous t - cell epitope, and two b - cell epitopes or conserved amino acid sequences from the two important molecules, ( 3 ) connecting them adequately through analysis by the molecule designing software. therefore, the synthetic new antigen may interfere with the process of fertilization by multiple ways and its contraceptive effects may be enhancing. based on the molecule designing methods, the b - lymphocyte cell epitope of sperm / testis specific protein sp17 and cyritestin which interfere with fertilization in mouse, as well as the promiscuous th cell epitope of the ribonuclease ( rnase ) in bovine were selected

    本研究以蛋白質分子設計的理論和方法研究避孕疫苗，將sp17和cyritestin關鍵表位和牛核糖核酸酶非選擇性th細胞表位合理組合，獲得新抗原- 35肽序列；並在合成、純化後分別與弗氏佐劑、免疫刺激復合物（ iscoms ）混合后免疫不同遺傳背景的雌性小鼠，觀察血清和生殖道內的特異性抗體滴度的動態變化、生育力的改變以及免疫后小鼠重要臟器的組織病理學改變：以及在ivf下，新抗原的特異性抗血清對精卵相互作用的影響及抗原在精子表面的特異性定位。
  8. Baculovirus / insect cell system has been widely used for recombinant protein production, but traditional system eventually resulted in cell lysis, so that the expressed recombinant protein was lost into medium

    摘要:桿狀病毒/昆蟲細胞系統已經被廣泛的應用在重組蛋白質的生產上，但傳統的桿狀病毒感染后會造成細胞溶裂，而使得表現出的重組蛋白質流失到培養基中。
  9. Get the inclusion bodies 2 ) western blot analysis of fusion protein expression ( 1 ) electrophoresis ( 2 ) transfer proteins from gel to membrane ( 3 ) blocking ( 4 ) incubation with primary antibody ( 5 ) enzyme conjugate incubation ( 6 ) substrate incubation. 3 ) gst detection module with cdnb enzymatic assay 3 purification of gst fusion proteins 1 the denaturalization of inclusion bodies. 2 purification using glutathione sepharose 4b column wash matrix with 1 pbs, prepare a 50 % slurry for batch purification method, pack column with matrix slurry

    三、 gst一hbrp重組蛋白的純化1 .超聲破碎細胞，離心，上清和沉澱進行sds一page電泳分析2 .樣品處理提取包涵體，變性后，加人用pbs平衡過的以utal腸onesepb抓脫4b ，室溫下孵育3 .以utadtionesepharose4b柱純化以utad雲onesepharose4b柱的準備根據毛山lel ，決定純化所需的gll衛ta1如oneseph娜se4b的柱床體積，用預冷的1xpbs清洗cldtathionese戶， se4b ，得到50 %的基質
  10. The recombination vectors were transformed into host strain of bl21 and induced with iptg. all the recombinant protein was expressed into inclusion body. the recombinant protein was identified with sds - page and westen - blot and purified through elution method. the muticlone antibody was got by immuning the rabbit with the purified protein

    把重組質粒轉化入表達受體菌bl21 ，經iptg誘導后都獲得了表達，且都以包涵體的表達形式存在，用sds - page和western - blot的方法對重組蛋白進行了鑒定。
  11. Hla - g1, which is a newly defined non - classical hla class i molecule, plays an important role in mediating immunotolerance and protecting embryo and even some kinds of tumors from nk cells attacking. the full - length coding sequences containing cdna of hla - g1 were cloned from placenta, monocytes and liver cancer tissue of chinese donors. sequence analysis reveals that it is a highly conserved human gene with only two amino acid mutation sites compared to foreign nationality. its truncated form was overexpressed in

    從中國人外周血單個核細胞胎盤組織和肝癌組織等樣品中克隆了包含完整hla - g1讀框的cdna與國外同行獲得的該基因及其蛋白質序列比較分析表明，該基因雖然有著細微的種族特異性，但高度保守並獲得了它的截斷型重組蛋白，根據蛋白一級結構和同源比較方法，模建了它及其與特異性受體kir2dl4形成復合體的空間結構模擬，預測了它們之間相互作用的特徵。
  12. Sds - page analysis suggested that the bacteria containing the recombinant plasmid pet - 32a ( + ) - igf - i produced the fusion protein of 30kda as it was induced by iptg. consisting 10 % of the total bacterial proteins, and the pet - 30a ( + ) - igf - ii produced the fusion protein of 14kda, which consisting 35 % of total bacterial proteins. 5

    Sds - page分析表明，重組質粒pet - 32a （ + ） - igf -在iptg誘導下表達分子量約30kda的融合蛋白，但其表達量不高，約為菌體總蛋白的10左右；重組質粒pet - 30a （ + ） - igf -在iptg誘導下表達分子量約14kda的重組蛋白，融合蛋白表達量約占菌體蛋白總量的35 。
  13. Biomicroarray is an important technique in the medical research at postgenome era, especially in the transcriptomic, proteomic and oncogenomic research

    摘要生物晶元是后基因組時代醫學研究中的重要手段，其在轉錄組、蛋白質組與腫瘤功能基因組研究中的作用日益突出。
  14. Alpha - chain protein was not demonstrated in the patient ' s serum, urine, diluted duodenal juice, and jejunal biopsy tissue

    但在病人的血清、尿液、十二指腸液、和空腸切片組織中無法顯示出甲種重鏈蛋白質。
  15. This paper describe the formation, isolation, denaturation and renaturation of recombinant proteins as inclusion bodies in e. coli, and summarize the most efficient ways to refold recombinant proteins

    摘要描述了大腸桿菌異源重組蛋白質的形成、制備、變性和復性，綜述了國內外變性、復性的有效方法。
  16. Potentially ast amounts of recombinant protein could be produced simply by increasing the planting area

    重組蛋白質的產量的增加可以簡單的通過增加種植區域來實現。
  17. Applications of animal ' s growth hormone are mostly studied by means of protein type, up to now, no other reports about gh gene directly used in animals have been found except one paper about the transfection of human gh gene into mice. in this research, we studied the changes in the bullfrogs after they are separately injected with the recombinant bullfrog gh protein ( re - bfgh ), bullfrog gh plasmid ( vb / gh ), grass carp gh plasmid ( vgcgh ) and expression vector vr1020 while the 0. 85 % salt - water as the control, for the purpose to determine the possibility of that the eukaryotic expression plasmid vbfgh and vgcgh are expressed in adult bullfrogs and affect their growth rate and plasma gh. we hope the results will help developing a new approach to promote the animal growth

    本研究首次以兩棲動物牛蛙為研究對象，進行了其生長激素基因的克隆以及原核表達質粒與真核表達重組質粒的構建、重組牛蛙gh蛋白的生物活性和免疫活性檢測以及重組蛋白制劑和核酸制劑的制備及其體內促長作用等表達效應研究，研究目的在於求證重組真核表達質粒是否能在牛蛙中表達、其促長效應是否強于重組bfgh蛋白，為探索重組生長激素真核表達質粒能否替代重組gh蛋白作為動物促長基因制劑等研究奠定理論基礎。
  18. Potentially vast amounts of recombinant protein could be produced simply by increasing the planting area

    重組蛋白質的產量的增加可以簡單的通過增加種植區域來實現。
  19. Enhancement of expression efficiency and solubility of recombinant protein in gene engineering by a new method

    提高基因工程中重組蛋白質表達效率及可溶性產物的一種新方法
  20. The recombinant plasmid was induced to express in bl21 host cell by imm iptg. the expressed protein is a 36kda fusion protein with six

    重組蛋白，這種內源性的apoes重組蛋白對25pmap25 35多肽誘導的神經成纖維膠質瘤細胞的死亡具有桔抗作用。
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