限制酶分析 的英文怎麼說

中文拼音 [xiànzhìfēnxī]
限制酶分析 英文
restriction enzyme analysis
  • 限 : Ⅰ名詞（指定的范圍; 限度） limit; bounds Ⅱ動詞（指定范圍, 不許超過） set a limit; limit; restrict
  • 制 : Ⅰ動詞1 （製造） make; manufacture 2 （擬訂; 規定） draw up; establish 3 （用強力約束; 限定; 管束...
  • 酶 : 名詞[生物化學] （生物體的細胞產生的有機膠狀物質） enzyme; ferment
  • 分 : 分Ⅰ名詞1. （成分） component 2. （職責和權利的限度） what is within one's duty or rights Ⅱ同 「份」Ⅲ動詞[書面語] （料想） judge
  • 析 : Ⅰ動詞1. （分開; 散開） divide; separate 2. （分析） analyse; dissect; resolve Ⅱ名詞（姓氏） a surname
  • 限制 : place [impose] restrictions on [to]; astrict; restrict; limit; confine; shut down on [upon]: 限制...
  1. It was suggested that eric - pcr could substitute for rapd in research related to the genetic identification and genetic diversity in auricularia and other edible and medicinal fungi : 2 to a certain extent, genetic differences among auricularia strains tested in this study did not have necessary relativity with their geographical origins respectively ; 3 in this study, genetic diversity in a. polytricha was higher than that in a. auricula : 4 in this study, a. fuscosuccinea had a higher homology to a. auricula than to a. polytricha ; 5 morphological characteristics validated the results from eric - pcr and provided a potential explanation for the higher similarity coefficient between a. auricular and a. fuscosuccinea ; 6 southern hybridization was employed by choosing a strain from a. auricula as a probe which hybridized with a. auricula and a. fuscosuccinea except a. polytricha, further confirming the veracity of the results from eric - pcr ; 7 in this study, isozyme analysis could not cluster the 7 strains from three auricularia species to different groups efficiently ; 8 2 strains from two auricularia species revealed high conservative degree and the restriction fragment patterns by 4 kinds of restricted enzymes showed no diversity

    本研究中，木耳屬2個種的2個菌株在its區域表現出較高的保守性， 4種限制型內切酶的酶切圖譜沒有顯示出多態性；增加內切酶種類及供試菌株數量，有可能獲得具有多態性的限制性內切酶酶切圖譜； 9本實驗中， its區域的真菌特異性引物與真核生物通用引物對于擴增效果無較大差異，擴增片段長度均為650bp左右； 10根據形態學實驗、 eric - pcr實驗以及southern雜交實驗的結果分析，紫木木耳屬種質資源的遺傳鑒定和遺傳多樣性評價耳極有可能是毛木耳種的一個變種; n .本研究中所用的gutc法是一種適用於木耳屬菌株基因組洲a快速提取的方法; 12 .傳統的形態學分類法和現代的分子生物學分類法，兩者的關系是相輔相成，互為驗證
  2. Based on the structure and function analysis of hirudin, a potent thrombin inhibitor, and some platelet aggregation inhibitors, which contain the recognition sequence argglyasp as their functional motif, two chimeric antithrombotic molecules were designed by introducing rgd sequence to hirudin cterminus. these chimera genes were constructed by pcr and inserted into the expression vector pet21a, the constructs were confirmed by restriction enzyme digestion and dna sequence analysis. these recombinant plasmids were transformed into

    經限制酶消化和dna序列分析，證明兩種重組質粒與設計完全一致。由於rgd -水蛭素嵌合基因上游連接了金黃色葡萄球菌蛋白a spa的信號肽序列，在iptg誘導下兩種嵌合分子都獲得了分泌表達，表達產物主要集中在細胞周質空間。
  3. In this paper, a field strain of infectious bronchitis virus was isolated from proventriculus tissue, morphological observation by electron - microscope and the biological characterizations of the virus were studied, pairs of specific primers are designed and synthesized in correspondence with them, according to the published sequences of infectious bronchitis virus three structural protein ( spike protein s membrane protein m nucleocapsid protein n ) genes, the cdna of si gene, s2 gene, m gene. n gene of ib v isolate lx4 were amplified by rt - pcr and full sequences were first reported

    在此基礎上，根據國內外已發表的ibv基因序列，分別設計特異性引物，應用不同引物進行反轉錄合成cdna ，分片段對ibv的主要結構基因進行pcr擴增，並分別將各個目的片段克隆到puc19載體上，在大腸桿菌dh5中實現目的基因的分子克隆，經藍白斑篩選、限制性內切酶分析、 pcr鑒定，篩選出重組陽性質粒，並對各個目的基因片段進行序列測定，從而獲得ibv主要結構基因全序列。
  4. Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2. construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr. the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e. co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )

    Mg _ 7重組噬菌體抗體庫的構建及鑒定從培養的mg _ 7雜交瘤細胞中提取並分離mrna ，反轉錄成cdna ；利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可變區基因，並通過? dna連接子將二者連接起來形成mg _ 7單鏈抗體基因；將mg _ 7單鏈抗體基因插入pcantab5e ；將連接產物轉化感受態tg1大腸桿菌，制備細菌形式的mg _ 7重組噬菌體抗體庫；通過菌落計數和限制性酶切分析（ ecor和hind ）評估mg _ 7重組噬菌體抗體庫的容量和重組率。
  5. Restriction enzymes and dna probes are used to determine the exact sequence, but it is possible to get a rough estimate by analyzing the frequency of recombination between the alleles of linked genes

    雖然可以利用限制性內切酶和dna探針精確的分析出特定序列，但是在分析連鎖基因等位基因間的重組序列時，會出現不準確的估計。
  6. After the plasmid was assayed for dna sequence, it was transformed into gs115 by electroporation

    利用限制性內切酶分析、 a序列測定在a水平對質粒進行鑒定。
  7. It indicated that the chinese isolates belong to north american group. two pairs of different primers of orf7 were used to identify the genotype of prrsv isolates in china, and then compared with the reference isolates of north american and european serotype and modified live prrsv vaccine. the results further proved that prrsv prevalent in china belongs to b genetype. combining the restriction enzyme digestion patterns obtained from mini, hindi and sactt, we observed 2 distinct rflp patterns

    在此基礎上，擴增各毒株的orf5基因，用mlu ， hinc和sac限制性內切酶切割orf5基因，通過這3種限制性內切酶獲得了各毒株的orf5基因限制性酶切圖譜，經rflp分析表明國內分離毒株與美洲型強毒株有著相同的rflp圖譜，而與疫苗毒的rflp圖譜存在明顯差異，進一步證明國內分離毒株的基因型屬於美洲型的強毒株。
  8. Methods isolates were identified as acinetobacter calcoaceticus by using antibiotic susceptibility test, plasmid profiles, restriction enzyme fingerprinting assay and plasmid elimination method

    方法對我院不動桿菌的感染進行調查，採用藥敏試驗、質粒抽提和限制性核酸內切酶分析法、質粒消除試驗。
  9. Primer - introduced restriction analysis - pcr, pira - pcr

    以引物引入限制性內切酶分析聚合酶鏈反應
  10. Then, the sensitivity of the subunits to trypsin traetment was compared by polypeptide pattern among these five psii preparations with sds - page

    分別用不同濃度的胰蛋白酶對這5種ps制劑進行限制性蛋白酶解處理， sds - page分析多肽組分變化。
  11. Two useful restriction endonucleases ( sspl and saci ) were choosed to type the different pathgenic ibdv strains. the result is saci only cleaved cibdv ( 4vaccine strains : bj836, b87, d78, bdc and 6 standard cibdv strains : hel, he2, he3, he4, sd3 / 98, zj1 / 98 ) and vibdv ( american variant - e ) rt - pcr products, whereas products obtained with vvibdv strains ( yl1, yl2, yl5, ylz ) were only cleaved with sspl

    選用具有分型意廬盧人聳2003屆碩十學位論文2義的兩種限制性內切酶（ saci和sspi ）建立的rea ，對5株屬于cibdv的疫苗株和6株標準強毒株；屬于vvibdv的毒株gx 、 yli 、 ylz和ylz等分離毒：屬于葉v的美國變異e株進行酶切分析，結果與前人的研究相符。
  12. Nucleotide acid sequence analysis indicates that two construts were correct

    對其進行了限制性酶切圖譜分析。
  13. Recovered the agarose and identified by agarose gelelectrophoresis. the producys of pcr fragments and pcambial303 plasmid ligation were transformed into e. coli ( dh5a ). the result of pcrof positive recombinant and restriction analysis demonstrated that the plant expressing vector of tps is attained

    Pcr產物經回收后，經瓊脂糖凝膠電泳鑒定后，與pcambia1303連接並轉化大腸桿菌dh5a ，陽性重組子經pcr和限制性內切酶酶切圖譜分析，表明已獲得海藻糖- 6 -磷酸合成酶基因的植物表達載體。
  14. Sinensis and e. j. hepuensis has been found in the sequences of the portions of 16s rdna and pcr / rflp studies of 110 samples, from six river valleys in eastern mainland of china. these subspecies - specific restriction sites allow rapid discrimination with the endonuclease dra i, and therefore can be used as a diagnostic genetic marker for identification of the two subspecies

    通過對中國大陸東部6個水系110個絨螯蟹個體16srdna部分序列的測定和pcr rflp分析，發現在合浦絨螯蟹與中華絨螯蟹之間存在3 4個固定的堿基替代，這種亞摘要種特異性的限制性位點可以通過限制性內切酶dra進行快速檢測，成為2個亞種的分子鑒定標記。
  15. I and hindlll respectively. after the digested products were run via agarose gel electrophoresis and transferred into nylon membrane, the southern blot was carried out using the cdna of rubber tree etrl as probe. the result of the southern blot showed that a hybridization band ( - 3. 0kb ) turned up from the ecor i digested product and another band ( - 4. 8kb ) turned up from the hindi ]

    從橡膠樹pr107品系的嫩葉提取基因組dna ，用限制性內切酶ecor 、 hinofll分別酶切，瓊脂糖凝膠電泳分離並轉膜后，用克隆的橡膠樹etri基因的cdna片段作探針進行southern雜交分析，結果表明， ecor酶切在約3 okb處有一條雜交帶出現， hi 。
  16. The point mutation in vp2 residue 297 of canine parvovirus from ser to ala acid was examined, which causes a alteration of the restriction enzyme aiu i site

    根據vp2位點297的改變引起限制性內切酶aiu酶切位點的變化，建立pcr依賴性限制性片段長度多態性分析（ prfl ）方法，檢測vp2位點297的變異。
  17. 374 - nt sequence analysis between nt 47 - 420 and restriction enzyme ( re ) clevage site mapping of f gene between nt 34 - 1682 were used to compare the 18 isolates for genetic analysis. a phylogenic tree was constructed based on the 374 - nt - sequence data of eighteen isolates in the study and 37 ndv reference strains from genbank and published resources

    通過dnastar軟體對f基因47 470nt間片段進行同源性分析比較並繪制了遺傳進化樹枝狀結構發生圖，結合334 1672nt間三種限制性內切酶（ re ： hinf ， bsto及rsa ）位點的分佈情況，確定了這些分離株的基因分類地位。
  18. The positive clone was screened and identified by pcr and analysis of restriction endonuclease digestion

    轉化感受態大腸桿菌dh5 ，經pcr及限制性內切酶分析得到陽性克隆。
  19. To screen the library, defferential screening had been employed. sixty - four clones were randomly picked to perform dot blot

    隨機挑出13個克隆進行限制內切酶分析，結果有10個質粒含有300一600bp的插入片段。
  20. Using a set of primers shared by endogenous and exogenous alvs ( a > b > c, d, e ), gp85 gene was amplified by polymerase chain reaction ( pcr ) from dna extracted from common cef and exogenous alv positive samples

    根據已經發表在ncbi的alv - a 、 b 、 e亞群的序列情況，分析多態性酶切位點，運用不同的限制酶bgl ， bamh ， ssp進行分型。
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