限制酶切消化 的英文怎麼說
中文拼音 [xiànzhìqiēxiāohuà]
限制酶切消化
英文
restriction digest- 限 : Ⅰ名詞（指定的范圍; 限度） limit; bounds Ⅱ動詞（指定范圍, 不許超過） set a limit; limit; restrict
- 制 : Ⅰ動詞1 （製造） make; manufacture 2 （擬訂; 規定） draw up; establish 3 （用強力約束; 限定; 管束...
- 酶 : 名詞[生物化學] （生物體的細胞產生的有機膠狀物質） enzyme; ferment
- 切 : 切Ⅰ動詞1 （合; 符合） correspond to; be close to 2 （用在反切后頭 表示前兩個字是注音用的反切）見 ...
- 消 : 動詞1 （消失） disappear; vanish 2 （使消失; 消除） eliminate; dispel; remove 3 （度過; 消遣） pa...
- 限制 : place [impose] restrictions on [to]; astrict; restrict; limit; confine; shut down on [upon]: 限制...
- 消化 : digestion; digest
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An infectious eiav clone was recovered by transfecting fatal donkey dermal ( fdd ) cell cultures and donkey leukocyte ( dl ) culture in vitro with the full - length gene clone of dv. the virus ( designed pd70344v ) derived from the third passage in dl culture was observed by electron microscope and the reverse transcriptase ( rt ) activity was determined
將包含全基因片段的三個基因克隆以限制性內切酶消化后順次連接克隆到載體ptz18r上，構建了4個全長基因的分子克隆，分別命名為pd30343 、 pd70333 、 pd70343 、 pd70344 ，其中兩個轉移到低拷貝載體plg338上，命名為plgd30343 、 plgd70344 。The genome could be digested only by restriction endonucleases hind, accl and xbal, and was resistant to cleavage by a wide range of other restriction endonucleases
病毒的基因組可以被hind 、 acc和xba酶切消化，但不能被其他所使用的限制性內切酶消化。But the results of restriction enzyme reaction are not satisfied. there may be two reasons which lead to these results : one is that the restriction enzymes are not suitable for this plasmid, that is to say there are no appropriate cut sites on the plasmid for these restriction enzymes ; and the other is that the purification of the plasmid is not enough for restriction enzyme reaction, and some impurity affects the last results
為了建立質粒的物理圖譜，我們對所提取的質粒進行了限制性酶切消化，摘要但是未能取得滿意的結果，原因可能有兩個：一，所選擇的酶不合適，在質粒上沒有合適的酶切位點；二，質粒的純度不夠，影響了酶切反應的結果。
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