類凝血酶 的英文怎麼說
中文拼音 [lèiníngxiě]
類凝血酶
英文
batroxobin-
Abstract : hirudin is the potent antithrombotic factor found in nature. hirulog is a dind of peptides designed by imitating hirudin
文摘:水蛭素是迄今發現的最強的天然凝血酶抑制劑，水蛭肽是模仿水蛭素與凝血酶的相互作用機制而合成的肽類化合物。Cloning and expression of the cdna of a thrombin - like enzyme from the venom of gloydius shedaoensis
大連蛇島蝮蛇類凝血酶基因克隆與表達研究The recombinant gloshedobin binding to the affinity adsorbent con a indicated that it is a glycoprotein
此重組類凝血酶在37t中性溶液中保存過夜將分解成小肽，但在0t下很穩定。Its amino acid sequence exhibits significant homology with those of other thrombin - like snake venom enzymes. based on the homology, the catalytic residues and disulfide bridges of gloshedobin were deduced to be as follows : his43, asp88, ser182 and asp176 ; and disulfide bridges, cys7 - 141, cys 28 - 44, cys 76 - 234, cys120 - 188, cys152 - 167 and cys178 - 213
以同樣的方法，得到長白山白眉蝮蛇（ gloydiusussuriensis ）毒類凝血酶基因，並比較了它與大連蛇島蝮蛇類凝血酶基因序列的差別，發現兩者的同源性高達98 ，只有三個氨基酸不同： gln ~ （ 66 ）Its enzymatic activity showed less similarity with gloshedobin. therefore, these three residues " replacement may influence the stability of the advanced structure of the enzyme
這兩類蛇毒類凝血酶基因的成功測序為其基因工程及其結構和功能的研究創造了條件。Compared with gloshedobin, gussurobin has three different amino acid residues : gln66 vs. glu66 ; gly117 vs. asp117 ; thr 225 vs. he225
兩種蛇毒類凝血酶的cdna序列及推導的氨基酸序列均為首次報道，並已申請了專利保護。Both the mature genes of gloshedobin and gussurobin were cloned into the vector pet - 32a ( + ), strain bl21 ( de3 ), to study their expression in prokaryotic cell. the gene was expressed under t7 promoter with a fusion protein partner of thx. tag and a 6x his. tag at its n - terminal. having been induced by iptg for 4 hours, the recombinant enzyme was examined in the cytoplasm by sds - page analysis
將大連蛇島蝮蛇和長白山白眉蝮蛇毒類凝血酶基因分別克隆到大腸桿菌表達載體pet - 32 （ a ） +中，在t7啟動子下表達出融合蛋白，融合部分為硫氧還蛋白，位於類凝血酶基因上游，並在其n端帶6xhistag標簽以利於表達產物的分離純化，經熱激轉化至宿主菌bl21 （ de3 ）中， iptg誘導斗小時后收獲菌體。The transfermants with highest resistance to g418 ( 4 g / l in ypd ) were screened to study their expression level in 150 ml shaking flask. having been induced with methanol for 36 hours, the target enzyme could be examined in the supernatant by measuring amidolytic activity
首次將大連蛇島賅蛇毒類凝血酶成熟基回克隆到表達載體ppicgk中，經電激轉化至畢氏酵母菌株gs15中，再經甲醇誘導，在150ml搖瓶畔1獲得細胞外分泌表達產物。According to the purity and the activity recovery, the recombinant enzyme was well purified by both of these two separation combinations. like natural gloshedobin, the recombinant enzyme exhibited strong esterase activity using tripeptide p - nitroanilide derivatives as substrate, but hydrolyzed n a - p - tosyl - l - arginine methyl ester ( tame ) or n a - benzoyl - l - arginine ethyl ester ( baee ) very weakly
與天然蛇毒類凝血酶一致，當用三肽p山itroanilide衍生物作為底物時，分泌表達的重組大連蛇島蝗蛇毒類凝血酶具有較強的生色底物活性，但用精氨酸甲酯如tame （ na廠tosyl l ar 。The ig class is iggi. mcab of at - iii provides very efficient tools for the detection and purification of at - iii
獲得三株分泌抗人抗凝血酶的雜交瘤細胞株d5 、 e2 、 f6 ，免疫球蛋白的類型為igg _ 1型。Today, only the last thrombin - like enzyme of the whole coagulation cascade and a synthetic peptide, attached to an aniline stain is used
今天，在整個階式凝固器和合成肽中，只有最後一種隸屬于苯胺污染的類似凝血酶的酶被使用。分享友人