bullfrog 中文意思是什麼

bullfrog 解釋
菜蛙
  1. Why does the bullfrog dig a trench to bring life - saving water to his tadpoles, while the mole rat expects his young to guard him with their lives

    相對地，河馬爸爸就粗心大意得多， ?們甚至會不小心地踐踏死自己的子女，也不動容。
  2. Blast holes in you muthafuckin tadpoles like a bullfrog,

    今夜你會不會來你的愛還在不在
  3. A bullfrog was croaking in the distance

    一隻牛蛙在遠處呱呱地叫。
  4. Jeremiah was a bullfrog. . bullfrog

    耶利米是一隻牛蛙. . .牛蛙
  5. Jeremiah was a bullfrog. bullfrog

    耶利米是一隻牛蛙.牛蛙
  6. Bullfrog 50 inflicts poison when your enemy damages you at short range

    牛娃50對近距離攻擊你的敵人造成毒傷。
  7. If you had, perhaps you could ' ve spent your wedding night as a bullfrog

    如果你喝了的話你就可能在新婚之夜變成一隻牛蛙
  8. When a tauren gets hexed and turned into a frog, does that make him a bullfrog

    當一個牛頭人被變成了青蛙，會不會是"牛蛙"
  9. There was no moon ; besides, there was not a sign of life around but the distant barking of a dog and the twang of a bullfrog from a neightboring marsh

    沒有月亮，除了遠處的狗吠和附近沼澤的蛙鳴之外，在也沒有任何生物的氣息。
  10. A team led by professor kim se - kwon of pukyong university in busan says it isolated a peptide with antioxidant properties from bullfrog skin

    釜山釜慶大學金勝勛教授領導的研究小組稱，他們從牛蛙的皮膚中分離出了一種具有抗氧化特性的生化肽。
  11. Applications of animal ' s growth hormone are mostly studied by means of protein type, up to now, no other reports about gh gene directly used in animals have been found except one paper about the transfection of human gh gene into mice. in this research, we studied the changes in the bullfrogs after they are separately injected with the recombinant bullfrog gh protein ( re - bfgh ), bullfrog gh plasmid ( vb / gh ), grass carp gh plasmid ( vgcgh ) and expression vector vr1020 while the 0. 85 % salt - water as the control, for the purpose to determine the possibility of that the eukaryotic expression plasmid vbfgh and vgcgh are expressed in adult bullfrogs and affect their growth rate and plasma gh. we hope the results will help developing a new approach to promote the animal growth

    本研究首次以兩棲動物牛蛙為研究對象，進行了其生長激素基因的克隆以及原核表達質粒與真核表達重組質粒的構建、重組牛蛙gh蛋白的生物活性和免疫活性檢測以及重組蛋白制劑和核酸制劑的制備及其體內促長作用等表達效應研究，研究目的在於求證重組真核表達質粒是否能在牛蛙中表達、其促長效應是否強于重組bfgh蛋白，為探索重組生長激素真核表達質粒能否替代重組gh蛋白作為動物促長基因制劑等研究奠定理論基礎。
  12. Main works and their important results are showed as following. firstly, the cdna encoding bullfrog gh is amplified by use of the total rna, extracted from adult bullfrog ' s pituitary, as the template, pi ( 5 ' - cggatcc atg gct tca ggg tta ggc ) and p2 ( 5 ' - cgaattc tta aaa ggt gca gtt gct ) as the primer pair and one particular cdna product, 660bp, was obtained after the rt - pcr - the product was purified by using dna agarose gel extraction method. after the purified cdna and pmd18 - t vector were ligated at 16 over night, the ligation products were transformed into e. coli strain dh5a and then the transformants were screened by clone pcr method

    首先，以成體牛蛙的腦垂體總rna為模板，進行rt ? pcr擴增得到約660bp的特異性pcr產物帶，切下瓊脂糖凝膠中的特異產物帶進行cdna膠回收，將cdna與pmd18 ? t載體進行t ? a克隆連接並轉化到dh5 e . coli后，進行菌落pcr 、質粒酶切鑒定，篩選出陽性菌株，測序結果經blast分析，與已報道的牛蛙生長激素基因高度同源，證實此陽性克隆為牛蛙生長激素基因轉化子，命名為pbfgh 。
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