engineered protein 中文意思是什麼
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Researches of schistosomiasis vaccines have gone more than 60 years, approximately including from the stages of dead vaccine and live vaccine ( irradiated attenuated cercariae vaccine ) to gene engineered vaccine, etc. many different forms of vaccines have been tested in animal models, including gluthathione s - transferase, paramyosin, irv - 5, triose phosphate isomerase, sm23, fatty acid binding protein ; which were considered promising by who / tdr. but none of them steadily accomplished the pre - set target level of 40 % protection. in order to enhance the protective capacity further, it is essential to develop novel vaccine antigens and / or vaccine adjuvants
血吸蟲病疫苗研究已有60多年的歷史，大致經歷了死疫苗、活疫苗（照射致弱尾蚴疫苗）和基因工程疫苗等研究階段，產生了一些who / tdr推薦認為很有希望的疫苗候選分子，如谷胱甘肽- s -轉移酶（ gst ） 、副肌球蛋白（ sm97 ） 、照射致弱疫苗抗原5 （ irv - 5 ） 、磷酸丙糖異構酶（ tpi ） 、曼氏血吸蟲膜內在蛋白（ sm23 ）和脂肪酸結合蛋白（ fabp ， sm14 ）等，但其對宿主的保護作用均不甚理想，未能穩定地達到40或以上的保護力水平，因此有必要繼續尋找新的疫苗抗原分子和/或疫苗佐劑，進一步提高其保護力。 -
The fusion protein was bactericidal active against staphylococcus aureus. in present study, we will truncate the none channel - forming do main, then attach the agrd to the pore - forming region ( k544 - i626 ) to construct a new engineered multidqmain protein machine - compact engineered peptide targeting staphylococcus aureus. such engineered peptide was constructed by linking the gene of staphylococcal agrd pheromone with the gene of c - terminal ( 1626 ) of colicin la pore - forming region ( k544 - i626 ) with site - directed mutation
利用點突變方法將金黃色葡萄球菌信息素agrd （ i型， ystcdfim ）的基因引入到大腸菌素fa梭基端1626基因上，並將限制性內切酶sacl酶切位點基因分別引入到大腸菌素fa的p4和k544上，通過酶切、膠回收、連接獲得含大腸菌素ia水性孔道結構域和金黃色葡萄球菌信息素agrd基因的重組質粒。 -
Treatment of avascular necrosis of femoral head by implanting a composite of bone marrow, bone morphogenetic protein, and noncelluar tissue engineered bone allograft
非細胞型組織工程化異體骨復合骨形態發生蛋白和紅骨髓治療股骨頭缺血性壞死 -
Green fluorescent protein has several good characters. under excitation of long uv light or blue light, it emits green fluorescence without requiring any exogenous substrates and cofactors. gfp gene expression can be used to monitor gene expression and protein localization in living cells and organisms. this is a development of revolutionary significance. the dna sequence of this gene can be re - engineered by mutagenesis and the gfp will get improved fluorescent properties. the applications of gfp will be wider and wider
綠色熒光蛋白具有優良的特性，在藍光或長紫外光的激發下，不需要任何外源底物或內源輔助因子的參入就能發出綠色熒光.綠色熒光蛋白基因的表達可用來監控活細胞或生物體中基因表達和蛋白質的定位.這是一個革命性的進展.而且，對基因dna序列的改造有可能使綠色熒光蛋白的發光特性更加優良，從而其應用范圍會更加廣泛 -
The engineered strain ghd - yz26 / e. coli bl21 ( de3 ) was incubated and induced at 30 for 8 hrs. the results show that the target fusion protein is soluble and active, which is about 15 % compared with the total proteins in cells, and the enzymatic activity reaches 5 u / ml that is about 8 folds compared with the activity of strain yz - 26
Colibl21 （ de3 ）中30誘導培養8hrs ，可獲得可溶性表達，融合蛋白的表達量約占總菌蛋白的15 ，其酶活力為5u ml ，約是原始菌株的d -海因酶表觀活性的8倍。 -
In conclusion, the compact engineered peptide can form lethal ion channel in the membrane of staphylococcus aureus guided by staphylococcal agrd pheromone, therefore the engineered peptide had bactericidal activity against staphylococcus aureus, especially against the drug - resistant strains. because of the properties mentioned above, the engineered peptide showed great potential in the future. the engineered peptide was an example of bactericidal protein machine combining two minidomains with different bioactivities and different protein origins
該工程多膚能在人工脂質膜上形成電壓依賴性離子通道，即當電壓由omv變為+ somv時通道立刻開放，電導驟然上升到某一數值，其後電導發生丫系列諧振式變化;而當電壓由+ somv變為一50mv時電導並不立即減小至o ，而出現正電壓狀態下類似的表現。 -
The standard way to manufacture protein medicines ? typically in huge fermenting vats filled with genetically engineered hamster ovary cells ? is labor - intensive
製造蛋白質藥物的標準做法是勞力密集的工業，一般需要巨型的發酵槽，裝滿了經遺傳工程改造的倉鼠卵細胞。
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