gel cell 中文意思是什麼

gel cell 解釋
膠體電池
  • gel : n. 凍膠，凝膠（體）；定型發膠。vi. （-ll-） 成凍膠，膠化。
  • cell : n 1 小室，單室；隔間，艙；〈詩〉茅舍；（單個的）蜂窩，蜂房。2 〈詩〉墓穴，墓。3 （大修道院附屬的...
  1. The metabolites eliciting inhibition to foam cell formation process of macrophage produced by endophyte hccb00017 were studied. several products were isolated through solvent extraction, and silica gel chromatography et al. one compound, hccb00017 - a, showed cytotoxicity ; the other two, hccb00017 - c and hccb00017 - e, showed inhibitory activity against foam cell formation process of macrophage

    對具有巨噬細胞泡沫化抑制活性的植物內生菌hccb00017的代謝產物進行研究，應用溶媒萃取、硅膠柱分離等方法，從其發酵液中分離出具有細胞毒性的活性物質hccb00017 - a ，以及具有巨噬細胞泡沫化抑制活性的組分hccb00017 - c和hccb00017 - e 。
  2. 二 、 effects of calcium concentration on the membrane - bound gq protein a subunit in the photoreceptor cell of macrobrachium rosenbergi on light adaptation and dark adaptation. the membrane - bound gq a was extracted from the retina of the macrobrachium rosenbergi with protein extract and was electophoresised by sds - page. the percent of the membrane - bound gq a was analyzed by tanon gis gel image disposal system

    二、鈣離子濃度對光暗適應羅氏沼蝦感光細胞膜結合gq蛋白亞基的影響用蛋白質提取液提取膜結合gq蛋白亞基並sds - page電泳，利用tanongis凝膠圖像處理系統分析其含量。
  3. ( 2 ) the mortality of cp cell in 10 were increasing directly related with the time in the low temperature. after 120d some cp cells died in the way of apoptosis. most nucleus of cells were condensed, and chromosome was marginated beside the nucleus inner membrane, cell sizes were reduced, dna ladder showed in dna gel electrophoresis

    （ 2 ） cp細胞系在10低溫下細胞死亡率與時間成正比， 120d的細胞具典型的凋亡現象，即細胞核固縮、染色體邊集在核膜內側；細胞體積變小；瓊脂糖凝膠電泳上顯示特徵性的「梯狀」帶。
  4. In order to detect the effect of human sperm mannose - ligand receptor on the fertilization ability, in the study reported here mannose - ligand receptors ( mrs ) were purified from human sperm by modified mannose - agarose gel affinity chromatography coloumn and determined protien concentration by lowry, preincubated zona - free hamster oocytes with four purified mannose - ligand receptor ( pmr ) concentrations before sperm penetration assay ( spa ) to test the pmrs cell biology nature of inhibition to fertilization

    本研究用改良后的親和層析法分離純化mr ， lowry法測定其蛋白質濃度，在精子穿透試驗（ spermpenetrationassay ， spa ）模型中定量研究其對精卵融合能力的影響並檢測其細胞生物學活性；以已知濃度的pmr （ purifiedmannose - ligandreceptor ）干預精子半透明帶試驗，觀察用pmr預處理半透明帶對精子與透明帶結合的影響。
  5. The approaches used in the study included : observing the microstructure and ultrastructure of the cell line of colossoma brachypomum ( cbt ) and the cell line of carp ( cp ) stressed low temperatures under fluoroscopy and tem ; analysis of dna damage in the cultured cells under temperatures stress by dna gel electrophoresis

    本研究採用的主要實驗方法：通過熒光顯微觀察、電鏡超微結構觀察確定cbt （淡水白鯧臀鰭細胞）和cp （草魚胚胎細胞）在低溫處理后的顯微與超微結構的變化。應用dna電泳分析細胞dna在低溫處理后的斷裂現象。
  6. Effects of estrogen on cisplatin - resistance human ovarian cancer cell line coc1 ddp : analysis by two - dimensional polyacrylamide gel electrophoresis

    順鉑耐藥的蛋白質雙向凝膠電泳圖譜分析
  7. In this paper, the effect of nuclear actin on the process of chromosome construction has been studied by utilizing the precise natural synchrous plasmodium of physarum polycephalum, sds - polyacryl amide gel electrophoresis ( sds - page ), western blotting, the cell - free system and optics microscopy. the major results and conclusions are as follows : 1

    本實驗以多頭絨泡菌原質團為材料，採用同步化培養、細胞核提取、 sds - page 、免疫印跡、非細胞體系構建、光學顯微鏡觀察等方法，研究了有絲分裂前期核內肌動蛋白對染色體構建的影響。
  8. After electrophorised on 1 % agarose gel, the pcr production was purified with agarose gel dna extraction kit. the segment was ligated with vector pmd18 - t and then was tranformed into the competent cell of dh5 a. a construction mstnd - pmd18t was generated by inserting the sequence of 254bp into pmd18 - t vector and selecting the sense clones. positive clone was identified by three ways : endonuclease digestion, pcr and sequencing. the result showed that the cloned sequence coincides with the designed sequence. this construction was digested with nco i and xho i and ligated the pet28a ( + ) vector digested with the same enzymes using dna ligation kit. the production of ligation reaction was transformed into the competent cell of bl21 ( de3 ). after 12 - 16 hours of culture, several colnes appeared on the plate. some positive clones were selected to extract their plasmid. these plasmids were digested by nco i and xho i and indentified by pcr. a contraction, mstnd - pet28a was generated. the result showed that the cloned sequence coincides with the designed sequence

    F _ 1長38bp ， r _ 1長36bp ，其它片段均40bp長， f _ 1和r _ 1片段兩端分別加上限制性內切酶nco和xho的識別位點序列。用成對單鏈片段進行延伸反應，然後用其他單鏈片段作為引物，進行pcr擴增，用dna快速純化回收試劑盒回收所得254bppcr產物，與pmd18 - t載體連接、轉化dh _ 5 。受體菌感受態細胞，利用藍白斑遺傳學篩選法篩選陽性克隆，提取其質粒，採用nco和xho雙酶切鑒定，獲得了254bp的片段；用pmd18 - t載體上的特異引物rv - m和m13 - 47進行pcr鑒定，獲得300bp的片段。
  9. Pavlova viridis, isochrysis zhanjiangensis and isochrysis galbana 3011 were cryopreserved in liquid nitrogen using encapsulation - dehydration. algal cells in early stationary phase were encapsulated in 3 % ca - alginate beads with 30 nacl, 2 million cells in one bead. beads were desiccated with silica gel then directly immersed in liquid nitrogen. the cell viability after warming was evaluated by chlorophyll content. the main factors influencing the cell viability, such as water content of beads, dehydration rate, dehydration procedure, preculture and recovery methods after thawing were studied. the results are as follows : 1

    本文以綠色巴夫藻（ pavlovaviridis ） 、湛江等鞭金藻（ isochrysiszhanjiangensis ）和球等鞭金藻（ isochrysisgalbana3011 ）等三種餌料金藻為試驗材料，用包埋脫水法進行冰凍保存。選擇靜止初期的藻細胞包埋在含有30氯化鈉的3 %的褐藻酸鈣膠球中，細胞負載約200萬個細胞/膠球，經過硅膠吸濕法脫水后，探討了膠球含水量、脫水速率、脫水程序、預培養以及化凍后恢復方法對冰凍保存存活率的影響。
  10. In order to understand the mechanism of mtx further and to investigate the genotoxic target organs, we studied the dna damage and the correlation with dose of mtx by using the alkaline single cell gel electrophoresis ( comet ) assay. liver, spleen, bone marrow, thymus, kidney, testicle, stomach and peripheral lymphocytes of mice were isolated at lh, 3h, 6h, 12h, 24h after 5mg / kg mtx intraperitoneal injection

    為了進一步了解甲氨蝶呤（ mtx ）的作用機制，探測其作用的遺傳毒性靶器官，為應用mtx治療過程中的臨床監測和副作用防治提供理論依據，我們以小鼠為研究對象，用單細胞凝膠電泳技術檢測了mtx腹腔注射染毒1h 、 3h 、 6h 、 12h 、 24h后對肝、脾、骨髓、胸腺、腎、睪丸、胃和外周血淋巴細胞的dna損傷作用及損傷程度與mtx劑量間的關系。
  11. In this study, the profiles of proteins expesssion in different growth phases of rt19 have been obtained using the technique of proteome analysis with two - dimensional polyacrylamide gel electrophoresis ( 2 - d page ). an efficient imagemaster 2d was used to reveal the number of protein spots corresponding from lag phase to stationary phase, varying from 398 to 516, which manifested kinetic state of proteome in cell

    本文採用高解析度的雙向電泳技術，得到不同生長時期的蛋白表達譜，應用高效的imagemaster2d軟體進行圖譜分析，結果顯示： （ 1 ）從延滯期到穩定期的末期，所表達的蛋白數量由398個逐漸增加至516個，顯示了細胞內的蛋白在不同生長期的動態表達水平。
  12. Protection characteristics of taurine on oxidative damage of human retinal pigment epithelial cells detected by single cell gel electrophoresis

    牛磺酸對經單細胞凝膠電泳技術檢測人視網膜色素上皮氧化損傷的保護特徵
  13. We applied single cell gel electrophoresis and cell culture technique, which constitute sing cell gel electrophoresis assay system for detecting mutagenicity to detect mutagenesis in vitro induced by animal drug quinocetone and olaquindox. and confirmed optimum lysing - time. vero cells in the period of logarithm - growth time were treated with 9. 1 ~ 273u mol / l h2o2 at 37 3h, then were lysed for lh, 2h, 3h and 4h to find optimum lysing - time

    並基於陽性致突變物h _ 2o _ 2作用於非洲綠猴腎vero細胞引起細胞dna損傷的原理，研究了其關鍵步驟-裂解時間，以9 . 1 273 mol l劑量的h _ 2o _ 2染毒處于對數生長期的vero細胞3h后，收獲細胞用於制備三明治凝膠板，分別裂解1h 、 2h 、 3h和4h並選擇最適裂解時間。
  14. The enzyme was purified from cell extract by ammonium sulfate fractionation ( 30 % - 80 % saturation ) and consecutive column chromatography using deae - cellulose and sephadex g - 100. the sod activity for purified enzyme could reach 4966 iu / mg proteins, and the molecular weight of the sod was about 20 kda which determined by sds - page electrophoresis. when the non - denaturing polyacrylamide gel stained with substrate for sod in the present of 0. 2mmol / l kcn or 0. 5 mmol / l h2o2, the active band was still showed at the same position, which indicated the sod could not be inhibited by the treatments with kcn or h2o2 and it was the mn sod

    通過sds一聚丙烯酸胺凝膠電泳可知該sod酶的分子量約為20kda .在非變性聚丙烯酞胺凝膠（ pagb ）電泳后，在凝膠son顯色反應液中加入0 . 2耐01 / lkcn或0 . 5inmol / lh202 ，凝膠sod顯色反應后在原來的sod酶部位仍然含有sod活性帶，這表明利用kcn和h20 :處理並不能抑制500的活性，該sod屬于mn一sod 。
  15. In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals

    首先，用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上，並轉化e colidh5a感受態細胞中，經pcr 、酶切以及測序證明得到了完整的2c3abc基因，並與國內外參考序列進行比較分析。然後，將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ，以zeocin ~ （ tm ）抗性篩選陽性克隆，大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞，通過zeocin ~ （ tm ）抗生素梯度濃度篩選，獲得重組酵母用0 . 5甲醇誘導表達，通過sds - page電泳、 westernblotting分析，結果表明， 2c3abc基因在畢赤酵母中成功表達，其表達產物為一95ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。
  16. Then the strain cell is detected to determine weather there is any plasmid in the cell. the detection result analysed by gelose gel electrophoresis shows that the cell harbors a plasmid, and this plasmid is relatively large

    應用質粒檢測的方法，對菌株細胞進行質粒檢測，並對檢測結果進行電泳分析，實驗發現，菌體細胞中存在一個質粒，而且質粒較大。
  17. Ectoplasm the outer gel - like layer of the cytoplasm in the cells of plants and some protoctists, which lies immediately beneath the cell membrane and contains a dense array of microtubules

    外質：植物及某些原植體細胞內，細胞基質近外方的膠狀層，直接位於細胞膜之下，並含有密集排列的微管。
  18. Study on ratio of moment move and ratio of inertia move as indices of single - cell gel electrophoresis

    矩類遷移比作為單細胞凝膠電泳指標的研究
  19. The components of 37kd and 39kd glycoproteins from gel were immunized respectively mice and polyclonal antiserums were obtained. glycoproteins of sperm cell surface can be examined using igg rabbit anti mouse - colloidal gold as probe

    用它們分別免疫小鼠得到多克隆抗體，以兔抗鼠igg膠體金15nm為探針，檢查此兩種蛋白在精細胞質膜上的分佈。
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