hybrid binding 中文意思是什麼
hybrid binding
解釋
雜化鍵聯-
In order to figure out the machanism of transcription control by ap - 2 a and to find the partner proteins which derectly binding to ap - 2 a, a yeast two - hybrid system by using ap - 2 a as a bait was performed to screen the hela cell cdna library
為了闡明ap - 2確切的轉錄調控方式，找出與其直接相互作用的蛋白質因子，我們以ap - 2為餌蛋白通過酵母雙雜交對hela細胞的cdna文庫進行了篩選。 -
To search proteins that associate with the mouse mint protein and regulate notch signaling in nuclei, and to study the function and mechanism of mint - mediated transcription repression, yeast two - hybrid assay was used to screen proteins that interact with a fragment of mint ( f5, amino acids 2226 - 2959 ). from 4x106 yeast clones transformed with the bait plasmid and a cdna library of 9 dpc mouse embryo, fifty - one were positive for nutritional screening and p - galactosidase assay. restriction digestion identified 10 independent positive clones and these were analyzed by dna sequencing these clones represent 3 correctly fused cdna fragments, which are mint, alpha a - crystallin - binding protein i ( alphaa - crybpl ), and nuclear receptor co - repressor 1 ( n - corl ), respectively
鑒于mwt基因編碼區較長，共有10799個堿基，故此我們將mint分為六段，分別命名為fi一f6 ，本研究以其中的f5 （ 222e959 ）和f6 （ 296s576 ）片段為研究對象，將h者分別插入pgb盯7載體中，結果顯示： mintfs可與核受體輔助抑制因子1階cori ） ， o晶狀體蛋白結合蛋白1hlphatcrybp入及mw加互作用，而f6可與igm的重鏈恆定區、泛素結合酶2l6和一個未知功能的新的蛋白基因進行結合。 -
The gpa1 gene was obtained via pcr amplification and was cloned in the two - hybrid dna binding domain vector pgbkt7 the combinant plasmid was designated as pgbkt7 - ga. - galactosidase assay indicated that got did not have the property of self - activation. after pgbkt7 - ga was transformed to yeast pj69 - 4a, we transformed arabidopsis vegetative tissue two - hybrid cdna library plasmids to yeast pj69 - 4a containing pgbkt7 - ga
通過pcr擴增得到gpa1基因並將其克隆到雙雜交dna結合域載體pgbkt7中，得到的重組質粒命名為pgbkt7 - g ， ?半乳糖苷酶活性鑒定表明g不具有自激活特性，將其轉化到酵母菌pj69 - 4a中，再以此為受體菌轉化擬南芥綠色營養組織cdna文庫質粒。 -
The hybrid control of a permanent magnet linear synchronous motor ( pmlsm ) servo - drive system using an adaptive recurrent neural network was put forward to solve the problem of poor stable precision in the servo system of packaging binding machine
摘要根據裹包機的驅動系統控制精度較差的問題，提出採用遞歸神經網路自適應混合控制線性同步電動器驅動機系統。 -
Pit13, an interactor binding to trpt1 protein, was screened by yeast two - hybrid, and confirmed by pull down and co - ip assays. the association of trpt1 with pit 13 demonstrated that trpt1 should probably participate in other activities besides in pre - trna splicing. more experiments will be required to determine the role of pit 13 protein
0酵母以雜交篩選、加衛工加wn檢測及ip實驗均證明m卜1 」且與功能未知的pit13蛋； 」之間存在著相互作用，說明imj基因除了參與trna剪接之外，還可能具有其它方面的功能，有待進一步研究。 -
Recently, two distinct genes, dreb1 and dreb2, encoding dna binding proteins that specifically bind to the dre sequence of rd29a gene had been cloned from arabidopsis by using the one - hybrid screening technique
它們編碼著與擬南芥rd29a基因的dre順式作用元件特異結合的轉錄因子，參與在乾旱，高鹽及低溫脅迫條件下的rd29a基因的表達調控作用。 -
The results of gst - pulldown and yeast two - hybrid showed kyot2 interact with them respectively in and yeast and vitro. 3 using the yeast two - hybrid system, we isolated a novel protein, kbp ( kyot binding protein ). three types of cdnas were identified by rt - pcr and were designated as kbp1, kbp2, kbp3, respectively
通過rtpcr我們獲得了kbp全長的cdna ，並發現它存在三種不同的剪接體ep 、 kbpz和ep3 ，同時通過計算機比較還獲得了它的基因組全長，這三種cdna和基圇組序列已經在g bank上登錄于邱。 -
To identify the interaction between kyot2 and zo - 2 - i3, yeast two - hybrid system, purification of kyot2 protein and gst pull - down assay were performed in the experiments. after kyot2 and zo - 2i3 exchanged their vectors, yeast two - hybrid test revealed physical binding of the two proteins
本實驗通過酵母雙雜交及gstpull downas腳驗證了kyotz蛋白與zoz蛋白在體外的相互作用，並通過酵母雙雜交實驗初步確定其相互作用的位點。 -
By yeast two - hybrid assay, aes was found to interact with gp130 intracellular region through its conserved q domain. results from the yeast two - hybrid assay, gluthione s - transferase fusion protein pull - down assay and immuno - co - precipitation assay indicated that the q domain of tle1 is capable of binding gp130 intracellular domain, and the intracellular membrane proximal region of gp130 containing conserved boxl and box2 motifs seemed essential for this interaction. to investigate the consequence of this interaction, tle1 - gfp fusion protein expression vector was constructed and co - transfected into nih 3t3 cells with gp130 expression vector
在通過酵母雙雜交分析確定aes通過q結構域與sp130分子胞漿區結合的基礎上，為確定tle1分子是否也能通過保守的q結構域與gp130分子胞漿區結合，我們通過pcr擴增編碼gp130胞漿區與tleq分子不同結構域的cdna ，構建了含有這些不同結構域的酵母雙雜交載體，通過酵母雙雜交分析證實： tle1分子通過其氨基端的q結構域與gp130分子胞漿區近膜段結合。
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