oocyte clamp 中文意思是什麼

oocyte clamp 解釋
卵母細胞電壓鉗
  • oocyte : n. 【胎】卵母細胞。
  • clamp : n 1 鉗,夾子。2 【機械工程】壓板,壓鐵;【建築】夾板;【造船】支梁板。vt (用夾鉗等)夾緊,夾住;...
  1. At first, the vrl - cdna plasmids were transformed into tg1 r. coli to enlarge vrl - cdna ; then the plasmids were extracted and cut off with enzymes ; subsequently, vrl - cdnas were transcribed into vrl - inrna by t7 or sp6 polymerase in vitro, following vrl - mrnas were injected into xenopus laevis oocyte to express into vr1 receptor protein ; at the end the vrl + - oocytes were tested by double electode voltage clamp

    這項實驗中,我們首先將大白鼠vr1 - cdna質粒轉入大腸桿菌中進行擴增,然後提取質粒並酶切,然後在體外轉錄成vr1 - mrna ,接著將vr1 - mrna注射到非洲爪蟾卵母細胞中表達為蛋白質受體,建立了實驗模型細胞,最後用雙微電極電壓鉗檢測此模型細胞。
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