protein assay 中文意思是什麼

protein assay 解釋
蛋白質定量法
  • protein : n. 【化學】朊，蛋白（質）。
  • assay : n 1 化驗；分析；鑒定，測定，驗定。2 被分析物，被化驗物。3 化驗結果，化驗報告。4 〈古語〉企圖，嘗...
  1. In order to get further evidence of the localization and distribution of emt - 1 in cells, we have prepared emt - 1 his6 - fusion protein and intend to abtain emt - 1 antibody for immuno - histochemistry assay

    為了進一步證實emt l在細胞內的定位及組織分佈，擬制備抗emt l抗體，進行免疫組化驗證。
  2. Application of hantavirus nucleocapsid protein into immunofluorescence assay

    漢坦病毒核蛋白在免疫熒光診斷技術中的應用
  3. To investigate the mechanism of trefoil factor 3 on the gastric intestine epithelial restitution, the recombinant human trefoil factor 3 was added to human colonic tumor cell and the proliferation effect was examined by mtt assay. the recombinant protein didn t promote the proliferation of the hct cells at low density of 0. 010. 05 g l and only has weakly proliferation effect at density of 0. 10. 2 g l. 1 g l of the recombinant protein could significantly promote the cell migration of hct cells when added to the monolayers cells

    將重組人三葉因子3 trefoil factor 3 ， tff3作用於人結腸腫瘤細胞，研究重組蛋白對細胞增殖的影響，結果發現該蛋白在較低的濃度1050 mg l下對細胞的增殖基本沒有影響，在100200 mg l濃度下該蛋白對細胞僅有微弱的刺激作用，提高濃度對細胞增殖作用沒有改變。同時研究了tff3對損傷的單層結腸腫瘤細胞遷移的影響，發現tff3對細胞有明顯的促進遷移作用。
  4. There were some degration in the purified protein, but gst - ap - 2 a still had the dna binding activity in the gel shift assay. the gst - testin and gst - antn1 were used for immunolize rabbits

    雖然所提取的融合蛋白gst - ap - 2出現較多降解，但是電泳遷移率變動分析顯示其仍然具有良好的dna結合活性。
  5. It was testified that the antibody can special immunological recognition the protein gst - cpti and cptl by indirect enzyme linked immunosorbent assay ( elisa ). the coefficient of correlation is significant and the potency is more than 1 : 800

    經間接elisa法檢測，抗體能與gst - cpti 、 cpti蛋白特異性結合，其相關系數達到顯著水平，效價1 800 。
  6. To search proteins that associate with the mouse mint protein and regulate notch signaling in nuclei, and to study the function and mechanism of mint - mediated transcription repression, yeast two - hybrid assay was used to screen proteins that interact with a fragment of mint ( f5, amino acids 2226 - 2959 ). from 4x106 yeast clones transformed with the bait plasmid and a cdna library of 9 dpc mouse embryo, fifty - one were positive for nutritional screening and p - galactosidase assay. restriction digestion identified 10 independent positive clones and these were analyzed by dna sequencing these clones represent 3 correctly fused cdna fragments, which are mint, alpha a - crystallin - binding protein i ( alphaa - crybpl ), and nuclear receptor co - repressor 1 ( n - corl ), respectively

    鑒于mwt基因編碼區較長，共有10799個堿基，故此我們將mint分為六段，分別命名為fi一f6 ，本研究以其中的f5 （ 222e959 ）和f6 （ 296s576 ）片段為研究對象，將h者分別插入pgb盯7載體中，結果顯示： mintfs可與核受體輔助抑制因子1階cori ） ， o晶狀體蛋白結合蛋白1hlphatcrybp入及mw加互作用，而f6可與igm的重鏈恆定區、泛素結合酶2l6和一個未知功能的新的蛋白基因進行結合。
  7. Get the inclusion bodies 2 ) western blot analysis of fusion protein expression ( 1 ) electrophoresis ( 2 ) transfer proteins from gel to membrane ( 3 ) blocking ( 4 ) incubation with primary antibody ( 5 ) enzyme conjugate incubation ( 6 ) substrate incubation. 3 ) gst detection module with cdnb enzymatic assay 3 purification of gst fusion proteins 1 the denaturalization of inclusion bodies. 2 purification using glutathione sepharose 4b column wash matrix with 1 pbs, prepare a 50 % slurry for batch purification method, pack column with matrix slurry

    三、 gst一hbrp重組蛋白的純化1 .超聲破碎細胞，離心，上清和沉澱進行sds一page電泳分析2 .樣品處理提取包涵體，變性后，加人用pbs平衡過的以utal腸onesepb抓脫4b ，室溫下孵育3 .以utadtionesepharose4b柱純化以utad雲onesepharose4b柱的準備根據毛山lel ，決定純化所需的gll衛ta1如oneseph娜se4b的柱床體積，用預冷的1xpbs清洗cldtathionese戶， se4b ，得到50 %的基質
  8. In this study, dna strand breaks in jm109 cells caused by - rays and the protective effect of lexa protein of deinococcus radiodurans have been studied by fluorometric assay of dna unwinding., the results indicated that the rate of dna strand breaks increased with the increase of - rays dose. however, when the concentration of lexa protein from 0. 1 g / ml to 10 g / ml, it still showed no protective effect on dna strand breaks, then lexa protein of deinococcus radiodurans was n ' t radioprotector

    本研究採用了dna解旋熒光法檢測由射線所致的dna鏈斷裂的損傷程度以及抗輻射菌lexa蛋白的輻射防護作用，在輻照前加入不同濃度的lexa蛋白，檢測dna鏈的斷裂，結果表明dna鏈的斷裂程度隨著射線輻照劑量的增加而增強，抗輻射菌lexa蛋白對射線所致dna鏈斷裂的修復不起作用，進一步說明抗輻射菌lexa蛋白不起輻射防護作用。
  9. With the base of related research on nucleic acid immunization, the technology was used to develop th e research and application of cpti transgenic plant. a new antibody preparation method by nucleic acid immunization to assay the expression of the transgenic gene was explored and compared with the traditional one which immune animal by protein

    在國內外大量核酸免疫的研究基礎上，本研究首次將核酸免疫技術應用於轉基因植物檢測研究中，探討一種核酸免疫法制備抗cpti抗體來檢測基因表達的方法，並與傳統的蛋白免疫方式制備抗體進行比較。
  10. The protein product of meq gene was highly expressed in the nuclei of recombinant baculovirus infected sf9 cells when using an anti - meq monoclonal antibody ( mcab ) 23b46 to run the immunofluorescence assay ( fa ) ; the expression quantity and if staining patterns differed with different times post - infection ( pi ). the results of western blotting and immunoprecipitation test showed there were two specific bands around 60 kd. the results of the study demonstrated that the baculovirus / insect cell system is effective to be used to express nuclear protein of virus

    結果發現:本表達系統產生的meq蛋白可被重組痘病毒表達的meq制備的單抗23b46所識別;在感染細胞中， meq蛋白僅局限於細胞核內，而且隨著感染后（ pi ）時間的增加，具有從核質向核仁和核膜轉移的趨向; w已stemblot和免疫沉澱試驗均證實重組桿狀病毒感染細胞裂解物中出現有兩條大小約為60kd的特異帶。
  11. Using kyot2 protein and antibody in gst pull - down assay similar result was also obtained. therefore we confirmed kyot2 interacted zo - 2 - i3 in vitro. furthermore it was identified in yeast that kyot2, through its lim2 domain, associated with zo - 2 - i3

    即zo4可以與kyotz的lm結構域發生特異性的結合，而不與kyoth的limi結構域或kyoti的limi及limz結構域相互作用。
  12. Was first processed by dgd embedment and embedment - free technique and general technique for em morphology. a perinuclear structure consisted of interacted filaments we called lamina - like structure was observed. then using western blot assay, we found a lamin - like component band of 68kd protein in the three - step - fractionated cells. to investigate the distribution of the lamin - like protein in cells, a immunofluorescence experiment for in situ hybridization was designed using goat anti - lamin protein antibodies as the probes. the results revealed that the positive reactivity presented at different part of the cells. the perinuclear cross - actions were distinct, and cross - action with the oral apparatus and the cortex were also obtained

    本文以dgd包埋去包埋技術對草履蟲的核纖層通過透射電鏡和免疫熒光分子雜交等技術進行了觀察。結果顯示，在核周存在由10nm纖維組成的核纖層免疫熒光結果表明，在核周呈陽性反應，並在其表皮口器等部位呈交叉的陽性反應蛋白分子雜交的反應帶在68kd處呈陽性反應。
  13. Dna and rna dot blotting revealed that the f gene was transcribed into mrna in the vero cells. there was expression of the f protein as shown by indirect immunofluorescent assay. the expression began at 48h post - infection and increased thereafter, as indicated by elisa

    將真核表達質粒pcdna3 - f高壓電轉化dam和phop基因雙突變的減毒鼠傷寒沙門氏菌zj111株（ zj111 / pcdan3 - f ） ，並直接轉染vero細胞，分別提取細胞總dna和總rna ， dig標記探針均可檢測到陽性雜交信號。
  14. Recombinant nucleocapsid protein of hantavirus as antigen for colloidal gold immuno - dot assay to detect hemorrhagic fever renal syndrome

    重組漢坦病毒核蛋白抗原用於免疫滴金技術檢測腎綜合征出血熱抗體的研究
  15. In this experiment, to screen the epitope of e2 protein of classical swine fever virus ( csfv ) defined by the monoclonal antibody ( mcab ) all, which had been prepared in previous experiment, the mcab all was raised in mice and followed by purification, the concentration of protein was assayed by using the bca protein assay kit

    本室利用e2基因疫苗制備了多株單抗，為e2的抗原表位研究提供了條件，我們以噬菌體隨機12肽庫分析鑒定了豬瘟病毒e2蛋白的抗原表位，為深入研究豬瘟病毒的抗原結構、制備更有效的診斷試劑和疫苗提供更多理論依據。
  16. The expression of lexa protein in the iptg - induced jm109 ( pza172 ) was checked by sds - page, and the survival curve of this strain was measured using colony formation assay after treatment with different doses of radiation and different concentrations of mmc

    應用電穿孔技術將攜帶有抗輻射菌lexa基因的重組質粒pza172轉入大腸桿菌jm109 ，其啟動子為lacz ，用iptg誘導， sds - page凝膠電泳檢測lexa蛋白的表達。
  17. Detection and bio - assay of insecticidal crystal protein bmb20 - 4 from bacillus thuringiesis

    4的殺蟲晶體蛋白檢測及毒力測定
  18. To identify the interaction between kyot2 and zo - 2 - i3, yeast two - hybrid system, purification of kyot2 protein and gst pull - down assay were performed in the experiments. after kyot2 and zo - 2i3 exchanged their vectors, yeast two - hybrid test revealed physical binding of the two proteins

    本實驗通過酵母雙雜交及gstpull downas腳驗證了kyotz蛋白與zoz蛋白在體外的相互作用，並通過酵母雙雜交實驗初步確定其相互作用的位點。
  19. In order to get the soluble recombinant eo protein and inspect the protein expression status convinently, the egfp and eo gene were ligated into baculovirus transfer vector. with the co - transfecting sf9 cells of baculovirus recombinant transfer vector and linearized viral dna, and plaque purification in the posttransfection procedure, the pure recombinant baculovirus were harvested, which infected the sf9 cells for amplifying to generate a p - l stock. in the meantime, the fluorescence microscopy detection indicated expressed egfp protein to confirm the heterogenous protein expression of recombinant baculovirus. the pi - stock from a pure plaque was used to generate a high liter p - 2 stock, which was determined in liter as 1. 14 107pfu / ml by performing a plaque assay. when a volume of p - 2 stock infected the sf9 cells with moi 5 - 10 for expression, the strong fluorescence was obeserved on the day 3 of postinfection

    此外，為了得到可溶性重組eo蛋白並便於觀察重組蛋白的表達情況，我們將egfp基因與eo基因相連插入昆蟲桿狀病毒轉移載體中，與線性桿狀病毒dna共轉染sf9細胞后通過噬斑純化得到純的重組桿狀病毒，將其感染sf9細胞制備p1種子液，同時用熒光顯微鏡觀察綠色熒光蛋白的表達情況剔除表達效果差的重組桿狀病毒。再用p1種子液感染sf9細胞制備高效價的p2種子液。通過病毒液的梯度稀釋和噬斑測定，確定p2種子液的病毒滴度達1 . 14 10 ~ 7pfu ml 。
  20. These results suggest that kyot plays an important role in the development of testis and spermatogenesis. 2 we performed yeast two - hybrid screening of a cdna library from human lymph nodes using kyot2 as a bait protein. 42 clones were gotten after 5xl06 were screened by four kinds of nutrition limitation and p - galactosidase assay, 22 clones were got after restriction of positive clones, at last, 13 genes were got by sequence assay including rbp - j known as the interacting protein with kyot before and two novel genes

    對此22個克隆進行序列分析，共獲得13種基因，其中包括已知的kyot相互作用蛋白rbpj和兩個未知基因，也包括在哺乳動物睪丸中特異3第四軍醫大學博士學位論文性表達的蛋白piasi ，同時還篩到了具有可與lm結構鞠互作用的pdzdomain分子緊密連接蛋白2和兩種同屬于kg家族的蛋白ringi和polycomb2
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