western blot 中文意思是什麼

western blot 解釋
蛋白法
  • western : adj 1 西的，西方的；在西的；向西的；從西方來的。2 〈W 〉 西洋的，西歐的。3 日落西山的，衰頹的。n ...
  • blot : n 1 墨污，墨漬，污點，污斑。2 瑕疵；恥辱，污名。3 〈古語〉塗去，抹去。vt （ tt ）1 用（墨水等）弄...
  1. Especially, at the site of implantation of day 5, 6, uterine stroma cell apposing the preimplantation blastocyst changed into the decidual cells and displayed a distinct immunostaining of phospho mapk. western - blot results showed p44 express stronger than p42 did, with both their peak expression were observed on day 5, during the period of uterine decidualization

    上述結果表明， ma衛k的激活與子宮內膜的接受性及蛻膜的形成有密切關系，推測mapk級聯通路激活對蛻膜化十分重要，蛻膜化可能與egf尼gfr直接或間接激活erk有關。
  2. The western blot analysis show that the antiserum induced by the new antigen plus hemocyanin could bind with the 22kd and 55kd proteins, which were existed in the testis tissue protein of mouse, rat and human. 2 ) the purified peptide emulsified in an equal volume of freund ' s adjuvant and immunized the female balb / c mice with 8 - 10 weeks old. the antiserums and the washings of vaginal membrane were detected by elisa, and shown the highest level of the specific antibody igg was 1 : 6000, while the iga was 1 : 300

    二、 p3多肽與弗氏佐劑混懸后免疫近交系balb c小鼠后，在血清和陰道粘膜沖洗液中可檢測出特異性lgg 、 lga ，最高效價分別達到1 ： 6000和1 ： 300 ；免疫后的小鼠脾臟淋巴細胞增殖率升高，淋巴細胞培養上清液中分泌的il 4和inf y也升高，且以il 4更明顯。
  3. Then. we analyzed the immunogenic proteins of aeromonas hydrophila, and identified 5 immunogenic proteins by the use of 2 - de and western - blot

    採用2 ? de與western ? blot相結合，對嗜水氣單胞菌的鼠免疫原性蛋白進行分析，鑒定了5個免疫原性蛋白。
  4. The cells were harvested and total e. coli proteins were detected by sds - page to select expressed strains. western - blot analysis of the gel that showed a band of similar size was the major protein immunoreactive with the anti - 6xhis mab

    Rpoifn經部分純化並充分復性后，用細胞病變抑製法測定rpoifn的抗濾泡性口炎病毒（ vsv ）活性。
  5. Nogo - 66 receptor, ngr, cloned in 2001, is a leucine - rich - repeat glycophosphatidylinositol - anchored membrane protein which mediates nogo - 66 inhibition of axonal outgrowth. both the long acidic amino - terminal domain and the nogo - 66 fragment have strong neurite growth inhibitory activity suggest that nogo - a has at least two inhibitory domains. northern blot, in situ hybridization, western blot and immunocytochemistry analyses show that in addition to oligodendrocytes, nogo - a mrna and nogo - a protein are also expressed in neurons in developing and adult brain and spinal cord, nogo - a is also found in peripheral organs such as heart and testis

    Northernblot 、原位雜交、 westernblot和免疫組化結果證明： nogo amrna和nogo蛋白除了在cns的寡突膠質細胞中表達，還表達于發育階段和成年的腦、脊髓和外周神經節的某些神經元中，在外周組織如睪丸和心臟也有表達； nogoe在cns和pns以及多種外周組織中有廣泛分佈； nogo （除表達于腦和心臟外，在骨骼肌中有較高表達。
  6. Over the induction process cell body became increasing spherical and retractile, exhibiting a typical neuronal perikaryal appearance. western blot a - nalysis indicated that cells exhibited increased expression of the neuronal marker nse after 5h of bfgf, atra treatment

    免疫細胞化學顯示在加人bme5小時後部分細胞胞體收縮變圓，胞質呈nse染色強陽性，細胞呈有較長突起的雙極，多極型。
  7. Salt treatment had effects on growth, succulence and some physiological parameters. in present study, suaeda salsa seedlings were treated with different salts and isoosmotic peg to examine the succulence and some physiological parameters. the hydraulic conductance ( lo ) of the roots, the water permeability of protoplasts and western blot analysis of aquaporins in plasma membrane and tonoplast under nacl were determined

    本實驗以鹽生植物堿蓬幼苗為材料，用不同的鹽及與nacl等滲的peg處理，測定肉質化及有關生理指標，並測定nacl處理下植物根的導水性，原生質體的水滲透性，並在分子水平上進行了細胞質膜及液泡膜水孔蛋白免疫雜交分析。
  8. To investigate whether ha 132 is a structural component of hasnpv, western blot analysis of proteins in budded viruses ( bvs ) and occlusion derived virions ( odvs ) was conducted

    對來自bv和odv的總蛋白質進行westernblot分析，結果表明ha132蛋白不是hasnpv病毒粒子的結構成份。
  9. Dab served as chromagen. western blot thirty micrograms of protein extracted from untreated and bfgf, atra - induced mmscs cultures were separated on a 8 % gradient acrylamide gel and eletrophoretically transferred to a nitrocellulose membrane. the blot was probed for nse expression

    4westernblot檢測誘導后細胞的nse表達情況從未經處理和經過bfgf ， atra誘導的細胞中提取30爬蛋白在8的sds一聚丙烯酸胺凝膠上電泳並轉移到硝酸纖維素膜上， 4 5脫脂奶粉封閉過夜。
  10. Ability to a - complement of the ea / ed protein was determined by the addition of onpg western blot test with rabbit to e. coli p - galactosidase pcab as first antibody was used to verify the fusion proteins

    以兔抗p半乳糖昔酶抗體做western blot以證實與gst融合表達的ea工d蛋白是p半乳糖昔酶的成分。實驗結果1
  11. The positive colonies that grew on the ampicillin ( amp ) plate ( lb agar medium contaning 100 g / ml amp ) were screened and identified. sds - page and western blot analysis were performed to study the expression profiles of target gene cein in e. coli

    從轉化的平板中篩選出陽性重組子，進行不同iptg濃度和不同誘導時間的表達研究，並利用sds - page電泳和westernblotting蛋白質印跡技術對外源基因cei _ （ 12 ）在大腸桿菌e
  12. After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s

    通過細胞的免疫組化，細胞裂解物的sds - page電泳， westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示：重組質粒轉染的細胞質中有棕褐色顆粒，而空載體轉染細胞及正常細胞無此現象；細胞裂解物sds - page電泳結果顯示：只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶，這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致； western - blot分析結果顯示：約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清，成蟲蟲體可溶性抗原免疫兔血清， ts87基因原核表達蛋白免疫兔血清（ ts87血清）以及一株具保護性的旋毛蟲單抗特異識別。
  13. Get the inclusion bodies 2 ) western blot analysis of fusion protein expression ( 1 ) electrophoresis ( 2 ) transfer proteins from gel to membrane ( 3 ) blocking ( 4 ) incubation with primary antibody ( 5 ) enzyme conjugate incubation ( 6 ) substrate incubation. 3 ) gst detection module with cdnb enzymatic assay 3 purification of gst fusion proteins 1 the denaturalization of inclusion bodies. 2 purification using glutathione sepharose 4b column wash matrix with 1 pbs, prepare a 50 % slurry for batch purification method, pack column with matrix slurry

    三、 gst一hbrp重組蛋白的純化1 .超聲破碎細胞，離心，上清和沉澱進行sds一page電泳分析2 .樣品處理提取包涵體，變性后，加人用pbs平衡過的以utal腸onesepb抓脫4b ，室溫下孵育3 .以utadtionesepharose4b柱純化以utad雲onesepharose4b柱的準備根據毛山lel ，決定純化所需的gll衛ta1如oneseph娜se4b的柱床體積，用預冷的1xpbs清洗cldtathionese戶， se4b ，得到50 %的基質
  14. We used sds - page and western blot to detect a - 1b glyco - protein moleculers in culture medium. one bind was detected at mole - culer mass between 66 - 43 kda, which show a maximal level at 7 day. discussion the function of human a - 1b glycoprotein precursor gene is still not known

    我們利用生長激素（ gh ）處理肝癌bel 7442細胞系， a ibgp的表達明顯高於對照組，說明gh可以調節a ibgp的表達，該基因可能參與gh的生理功能及在與gh相關的疾病中發揮作用。
  15. The fragments were ligated directly to the pichia pastrois expression vector ppic9 to got ppic9 - e3and ppic9 - e8. vectors were amplificated in the e. coli dh5 a and were linearized with bgl ii. the linearized vctors were transformed into host strain gs115. the recombinated strain was selected though phynotype and pcrthe positive strain was induced with methyl alcohol and was selected by dot - elisa. the recombinated protein was detected with sds - page and western - blot as before

    重組菌用甲醇誘導表達，用dot - elisa的方法篩選到表達量較高的菌株。將篩選出的菌株大量的誘導表達，對表達上清處理后，用sds - page和western - blot進行鑒定。同時，用hiprep16 10heparinff肝素親和柱對表達蛋白進行了初步的純化。
  16. Rt - pcr results suggested ha94 was a late gene. western blot analysis of extracts of hasnpv - infected hzaml cells revealed a specific protein of 43 kda from 48 h to 96 h p. i.

    Rt - pcr結果表明ha94是一個晚期基因，在感染后24小時開始檢測到病毒的轉錄產物，持續到表達至96小時。
  17. Western - blot analysis showed the expressed protein was specific to antisera against prv fa strain. the ge - elisa for differentiation of prv infected from vaccination was developed using the expressed ge protein as antigen

    Coli中高效表達的偽狂犬病病毒ge蛋白為抗原，以辣根過氧化物酶（ hrp ）標記的spa為二抗，建立了ge - elisa鑒別診斷方法。
  18. In order to study the expression of 3 - defensins in liver as acute phase response proteins, a murine systemic acute phase responsive model was established by intraperitoneal injection o f lipopolysaccharide ( lps ) in our study. the mbd3 cdna sequence ( 145 - 169 bp ) was labled with [ - 32p ] atp as a probe to detect mbd3 mrna in different tissues by northern blot and analyze the time - and dose - dependant expression caused by lps. the 5 " flanking sequence ( - 167 - 179 bp ) of the mbd3 gene was designed as the probe and labled with [ - 32p ] dctp to investigate the binding of transcriptional factors to this region by electrophoretic mobility shift assay ( emsa ) and south - western blot

    以小鼠mbd3基因145 ? 169bpcdna序列合成探針，經[ - ~ （ 32 ） p ] atp標記后通過northernblot方法檢測mbd3在肝臟中的表達，同時分析了mbd3基因誘導表達的組織特異性，劑效和時效關系；結合mbd3基因啟動子區序列分析，以- 164 ? - 179bp雙鏈dna序列合成探針，經[ - ~ （ 32 ） p ] dctp標記后通過電泳遷移率改變實驗（ emsa ）和south ? westernblot方法對參與mbd3在肝臟中誘導表達調節的轉錄因子進行分析。
  19. The results of emsa showed the obvious retardant bands, which suggest that some proteins in nuclei can bind to the specific dna sequence of mbd3 gene after lps treatment. 5. the molecular mass of this dna binding protein was assessed between 43. 0 - 66. 2 kd by south - western blot

    4 .電泳遷移率改變實驗有明顯的滯后帶形成，表明lps刺激后，肝臟組織的細胞核內有轉錄因子與mbd3基因特異的dna序列結合，參與mbd3基因的誘導表達5 . south一westemblot進一步確定此轉錄因子分子量在43 . 0一「 . 2kd之間。
  20. Labeling tunel method. the cell ultrastructural changes were similar to apoptosis in animal cells : the apical meristemetic cells underwent the programmed cell death. this was first detected in the apex cells of apical meristem, while peripheral cells differentiated gradually into different parts of a floral bud. but all the cells in the floral bud were subjected to the pcd process before it developed into a complete flower. 140bp dna fragment was found to deposit in apical bud during the plant development. the most important role of caspase - 8 was detected by western blot, and the expression of the procaspase - 8 was time - related with the dna frgmentation and the transformation from vegetative to the reproductive growth. these results suggested that pcd was an active process during the differentiation of apical meristem, and the senescence observed in the apical bud was due to the pcd process

    顯微超微結構研究表明，短日照條件下豌豆頂芽的衰老過程是從營養生長錐向花芽的轉化，而用dna原位末端標記tunel caspase - 8 western blot和140 bp dna片斷積累的試驗結果證明，轉化為花芽的整個生長錐細胞發生了編程性死亡pcd ，而且其最頂端部分細胞首先發生pcd ，而頂端周圍的分生組織細胞逐漸分化出花芽的各部分，但頂芽最後並沒有發育成為完整的花，所有細胞就都發生pcd ，從而頂芽衰老。
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