桿體細胞 的英文怎麼說
中文拼音 [gǎntǐxìbāo]
桿體細胞
英文
rod cells-
Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
利用從國外引進的新城疫熱穩定性天然弱毒b _ ( 95 )株接種spf雞胚繁殖病毒,經處理后提取病毒的基因組rna ,參考國內外發表的ndv融合蛋白基因序列,設計一對特異性引物,經反轉錄聚合酶鏈式反應( rt - pcr )擴增出約1700bp大小的特異性片段,將此片段回收純化后,利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中,再轉化大腸桿菌jm109感受態細胞,轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss
將缺失型pap基因克隆到植物表達載體pbi121中,通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中,然後採用葉盤法,在該農桿菌的介導下將pap基因導入普通煙草中,經過卡那黴素抗性篩選,最後獲得了轉pap基因的工程煙草植株,摩擦接種煙草花葉病毒( tmv ) ,與非轉基因煙草相比,能夠推遲癥狀表現達2月之久,說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。The bacilliform cell penetrate into interior of the fibre to degrade the cellulose strongly and produced a mass of sticky polysaccharides. after cultured 48 hours, the bacilliform cell ' s surface of sporocytophaga have a great change. at this stage the bacilliform produce a lot of sticky polysaccharides. these sticky polysaccharides associated with the sites where the filter paper was decomposed intensively and form thorns on the surface of the bacillium. at the same time, the filter - paper weight loss is the greatest and decomposing rate is the fastest, so we think that the sticky polysaccharides are produced during the cellulose degradation
培養48小時,桿狀細胞的表面結構發生很大的變化,此時的菌體表面已產生大量的粘性多糖,這些粘性多糖因菌體在纖維素表面滑動而在菌體表面形成突起,即在纖維素被旺盛降解部位的菌體表面產生了大量突起;而產生突起的菌體深入到纖維素分子內部,纖維素表面可以清晰地看到由於菌體嵌入纖維素分子內部而留下的凹陷。A new transgenic system of gerbera mediated by agrobacterium tumefaciens
小細胞團為受體的根癌農桿菌介導的非洲菊基因轉化體系The hemagglutinating activity of the fluids was not affected by treatment with 50 mm edta and egta, while it was increased by treatment with 3 - me
經注射雞大腸桿菌后,文昌魚體液中的凝集素對人b 、 o型紅血細胞,兔、草魚和蟾蜍紅血細胞的凝集活性明顯增強。Sub - - clone of s, . / hbsag fusion gene : pbuescripts, . / hbsag and ppiczaa were digested separately by xhoi and xbai enzyme, and were linked under t4 dna ligase, ppiczaa s, / hbsag was constructed and transformed to e. coli
Hbsag質粒與ppiczaa載體分別經xhol和xbaln切,再在t4dna連接酶作用下進行連接,獲得工程菌表達型ppiczaas ; hbsag質粒,轉化大腸桿菌t0p10細胞,經xhol和xbal與sacll和xbal酶切電泳,證實s ; 。The recombinant plasmid puge dna and transfer vector pfastbacl dna were treated again in the same enzyme, were linked by means of t4 dna ligase and transformed into e. coli jm109 permissive cells, yielding recombinant transfer vector plasmid pfastbac - ge dna and were transformed into dhlobac containing vector bacmid
將重組質粒pugedna與轉移載體pfastbacldna用bamhi和ecori雙酶切處理, t _ 4dna連接酶連接,用連接產物轉化大腸桿菌jm109感受態細胞,得到重組轉移載體質粒pfastbac - gedna 。Our study results indicated : on light adaptation, the ratios of the density of the gold particles in the rhabdom to that in the cytoplasm in macrobrachium rosenbergi photoreceptor cell in high calcium solution, physiological solution and low calcium solution was 21 / 6 、 1
結果顯示:光適應組,在高鈣溶液、生理溶液和低鈣溶液中細胞質與感桿束中膠體金密度的比值是21 / 6 、 17 / 8和13 / 14 。The bacteria - bursting enzymes that caught gasson s attention are called lysins. different lysins attack specific bacteria and the bacteriophage lysins researched by gasson s team can be used to detect or selectively kill listeria and clostridium
不同的細胞溶解酶攻擊特定的細胞,加森小組所研究的噬菌體細胞溶解酶可用於探測或選擇性地殺死李斯特氏菌和梭狀芽孢桿菌。The results showed that the fine structure of the photoreceptor, the diameter of rhabdom, the dimension of perirhabdom vacuole, the number of pinocytotic vesicle below the microsvilli, the location of pigment granules, the emergence of lamellar bodies and lysosomes in cytoplasm, were different in light and dark adaptation
結果顯示在感桿束的直徑、膜下瀦泡囊的體積、微纖毛基部的胞飲泡數量、色素顆粒的位置以及有無脂滴、板膜體和溶酶體等細胞器方面,光適應和暗適應的光感受器有著明顯的差異。In vitro study of peripheral blood polymorphonuclear leukocyte degranulation induced by the filtrate of ultrasonic pulverization from actinobacillus actinomycetemcomitans
伴放線放線桿菌超聲粉碎濾液誘導外周血中多形核白細胞脫粒的體外研究Quinic acid, used shikimate pathway in e. coli, it is necessary to extend metabolic pathway by introduction of a heterogenous gene qutb into the host cell. double specific enzyme genes arog, qutb or three ones arog, qutb, arob were co - expressed in a single plasmid pbv220 to improve the enzymes " rate - limiting reactions. modifications of e. coli chromosome by both disruption of the arod gene and directed - site insertion of the arob gene resulted in the change of carbon flow redirected into the quinic acid biosynthesis branch
利用大腸桿菌莽草酸途徑合成新的代謝物奎尼酸,須在宿主細胞引入異源酶基因擴展代謝途徑;串聯表達酶基因,同時適量增加不同種屬的多個關鍵酶酶量,改善限速反應;利用同源重組進行基因整合和基因破壞,改造染色體結構定向改變微生物代謝途徑;目的是將碳代謝流最大程度的引向奎尼酸生成的方向。Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2. construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr. the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e. co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )
Mg _ 7重組噬菌體抗體庫的構建及鑒定從培養的mg _ 7雜交瘤細胞中提取並分離mrna ,反轉錄成cdna ;利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可變區基因,並通過? dna連接子將二者連接起來形成mg _ 7單鏈抗體基因;將mg _ 7單鏈抗體基因插入pcantab5e ;將連接產物轉化感受態tg1大腸桿菌,制備細菌形式的mg _ 7重組噬菌體抗體庫;通過菌落計數和限制性酶切分析( ecor和hind )評估mg _ 7重組噬菌體抗體庫的容量和重組率。( 2 ) the chromosomes of bmn cells showed the typical characteristics of lepidoterati insects. chromosomes of matephase without apparent centromere are short pole - like and pellet - like
( 2 ) bmn細胞的染色體表現為典型的鱗翅目昆蟲的染色體:中期染色體短桿狀或顆粒狀、無明顯的著絲粒。The reasults are summed up as following : 1 the study on chromosomes and mitoses of bmn cells the cell line, bmn, is a silkworm cell line widely used in silkworm molecular genetics, cell engineering, gene engineering and baculovirus expression system but whose genetics and cytobiology studies are nearly untouched. the chromosomes and mitoses of the bmn cells are researched by the air - drying method and culturing cells on cover glasses
同時,還通過原代培養實驗對新的家蠶胚胎細胞系的建立進行了探索和嘗試,並對家蠶胚胎原代培養過程中出現的細胞和組織類型進行了觀察、探討與研究。 1bmn細胞有絲分裂及染色體研究bmn細胞是家蠶分子遺傳學,細胞工程、基因工程和桿狀病毒表達系統中廣泛應用的家蠶細胞,但其遺傳學和細胞生物學背景知之甚少。A database search revealed that the putative sequence of the red gene shows 40 - 50 % identity with those of uroporphyrinogen iii methyltransferase ( encoded by coba gene ) from various kinds of bacteria. an over - expression of the coba gene in e. coli was reported to lead to an accumulation of trimethylated derivative of porphyrin termed trimethylpyrrocorphin and factor ii, which emit strong red fluorescence under uv
在ddbj中搜索到多種細菌來源的coba基因(編碼uroporph仰nogenhmethyltransferase )與redsene有40 50的同源性,並據報道,其中一個來源於pmpboibaclerilllaslldelll切chit的coba的基因,轉人大腸桿菌、酵母菌及動物細胞后能使表達載體在紫外線下發射紅色熒光。Meq protein, highly expressed in the insect cell line sf9 by the baculovirus vector was immunized into balb / c mice and the immunized spleen cells were collected and fused with the tumor cell line sp2 / 0 via peg - 1000 in vitro. the hybridoma cells were cloned and screened for the ability of anti - meq mcab secretion by fa with the mdv ga infected chicken embryo fibroblast ( cef )
利用通過桿狀病毒載體在昆蟲細胞系sfg上高度表達的meq蛋白產物免疫balb / c小鼠,然後收獲其免疫脾細胞並與腫瘤細胞系spz / 0通過peg于體外融合;獲得的雜交瘤細胞被克隆並通過與mdv感染的雞胚成纖維細胞( cef )做免疫熒光試驗( fa ) ,進行其分泌抗meq單克隆抗體( mcab )能力的篩選。Results we construct recombinant angiostatin baculovirus with a high virus titer ( 2 + 108 pfu / ml ) successfully. recombinant angiostatin was effectively expressed in insect cells ( sf9 ) as 53 kd fusing protein and its expression level was about 90 % of insect cellular total soluble proteins. the recombinant angiostatin protein could inhibit endothelial cell proliferation in vitro with ic50 value of 2
實驗結果我們成功地構建了滴度高達2x10 『 pm llil的angiostatin重組? 2 ?桿狀病毒,並在昆蟲細胞sffi中高效表達了分子量為53kd的an giostatin重組蛋白,重組angiostatin蛋白不僅在體外顯著抑制內皮細胞的生長, k 。The vegetative cells of the nust03 are rods and the spores are spherical
菌株的營養體細胞桿狀,粘孢子球形。In the experiments discussed in chapter 5 we generated two recombinant viruses based on an acmnpv - and hasnpv - bac - to - bac system, respectively. in such recombinant viruses the busuctl gene under polyhedrin promoter was inserted into polyhedrin gene locus. a preliminary bioassay was conducted
第五章利用桿狀病毒bac - to - bac系統構建了含有油桐尺蠖核多角體病毒的類蝸牛毒素基因的重組病毒racbacctl和rhabacctl ,在其相應宿主甜菜夜蛾和棉鈴蟲的細胞水平和蟲體水平進行了超表達實驗。分享友人