溫和噬菌體 的英文怎麼說
中文拼音 [wēnhéshìjūntǐ]
溫和噬菌體
英文
temperate bacteriophage-
The wells of elisa plate were coated with pab ( 100ng / l ) against h3n2, then phage was added to the wells. after incubation, the wells were washed vigorously with tbst to remove nonbinding phage. phage bound to the antibody were eluted with 0. 2mol / l glycine - hcl ( ph2. 2 ) for 10 min at room temperature and neutrialized with 2mol / l tris - hcl ( ph9. 1 )
以抗h3n2流感病毒的多克隆抗體（ 100ng l ）包被酶標板，加入制備好的肽庫，用tbst洗去非特異結合的噬菌體，加0 . 2mol l甘氨酸-鹽酸（ ph2 . 2 ） ，室溫放置10min以洗脫特異結合的噬菌體， 2mol ltris - hcl （ ph9 . 1 ）中和后，取2 l噬菌體接種大腸桿菌xl1 - blue菌進行空斑滴定，其餘噬菌體擴增後用于下一輪篩選，共重復3輪淘洗。Using these vectors, expression of the pks gene was achieved both in streptomyces lividans and in e. coli in a heat - dependent manner, suggesting that the lambda promoter and temperature - sensitive lambda represser functioned in s. lividans as well as in e. coli
利用這些質粒在變鉛青鏈黴菌和大腸桿菌中均表達出pks蛋白，兩種宿主中的表達都是熱依賴的。暗示噬菌體啟動子和溫敏型阻遏物在變鉛青鏈黴菌和大腸桿菌中都具有功能。
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