等位基因重組 的英文怎麼說

中文拼音 [děngwèijīyīnzhòngzǔ]
等位基因重組 英文
interallelic recombination
  • 等 : Ⅰ量詞1 （等級） class; grade; rank 2 （種; 類） kind; sort; type Ⅱ形容詞（程度或數量上相同） equa...
  • 位 : Ⅰ名詞1 （所在或所佔的地方） place; location 2 （職位; 地位） position; post; status 3 （特指皇帝...
  • 因 : Ⅰ動詞[書面語] （沿襲） follow; carry on Ⅱ介詞1 [書面語] （憑借; 根據） on the basis of; in accord...
  • 重 : 重Ⅰ名詞（重量; 分量） weight Ⅱ動詞（重視） lay [place put] stress on; place value upon; attach im...
  • 組 : Ⅰ名詞1 （由不多的人員組成的單位） group 2 （姓氏） a surname Ⅱ動詞（組織） organize; form Ⅲ量詞（...
  • 重組 : bpr
  1. But as a kind of mature management system, it has n ' t been applied successfully in most of chinese companies. the reasons of that include misunderstanding of science property of overall budget management 、 unfit budget management organization 、 lack of scientific management methods, and another important reason is that most of companies use the traditional building method of budget management system. the traditional building method based on the company ' s existing department functions, keeping the existing management process and work process fixedness, and expressing the department ' s work plan in quantity or currency form as their budget

    然而全面預算管理作為一項比較成熟的管理體系，在我國大部分企業卻並沒有得到很好的實施，這其中固然有對全面預算管理科學性認識不足、預算編制工作的組織不到位、缺乏科學的預算管理手段和控制手段等原因外，另一個重要原因是大部分企業在構建全面預算管理體系時使用的是傳統的構建方法，即基於企業現有的部門職能劃分而進行的，在保持作業流程和管理流程不變的情況下，將部門工作計劃以貨幣或數量的方式表示出來，即成為公司預算。
  2. Using the net assets per capital, the investment return rate, the t - m model, the h - m model, the single factor evaluating model which consists of the treynor index, the jensen index, the sharpe index and the square m index, we evaluate the performance of the twelve mutual funds. and we come to the following conclusions : ( 1 ) after the modification of the risk factor, our mutual funds in the recent one year outguess the market ; ( 2 ) better performance comes from the aid of the government, the improvement of the investment environment and the hard, smart work of the managers especially in the way of selecting some securities in the capital market. ( 3 ) though we make progress, there are still many problems which prevent the further development of our mutual funds such as the devise of the management fee and the characteristics of different funds, all of them divided into the subjective ones and the objective ones

    通過使用投資基金單位凈資產和投資收益率指標、單因素整體績效評估模型，包括treynor指數、 jensen指數、 sharpe指數和業績的m ~ 2測度以及t - m 、 h - m模型對12隻樣本基金進行實證研究，實證研究表明： （ 1 ）經過風險調整后，在最近的一年中，我國證券投資基金的業績總體上優於市場基準組合； （ 2 ）基金業績的提高得益於管理層的重視、投資環境的改善和基金經理的經營，而基金經理的良好業績是通過一定的證券選擇來獲得的； （ 3 ）已成為證券市場上舉足輕重力量的基金在發展過程中雖然取得了一定的成績但其進一步發展還面臨著許多問題，有主觀存在的諸如管理費率的設定、基金風格方面的問題等等，也有客觀存在的諸如證券市場現階段的不完善等等，所以，我們應該抓住《證券投資基金法》問世帶給基金業發展的契機，大力促進證券投資基金規范發展，採取各種措施做大、做優和做強基金業。
  3. Owing to its excellent adaptability to large angle - displacement between two transmission shafts and other characteristics such as large transmission ratio, terse mechanism, high transmission efficiency, cardan cross - pin spindles has been widely used as an important mechanical part in transport machines, metallurgy machines, petrolic machines, heavy machines, etc. as an effective and promising approach to resolve complicated development of modern software, component - based development ( cbd ) is put forward to change the present way of software development by producing software through assembling ready software components and its final target is to realize the industrialization of software development totally

    十字軸式萬向聯軸器能夠很好地適應傳動軸間的較大角位移，而且具有傳遞扭矩范圍大、結構緊湊、傳動效率高、維修保養方便等特點，是一種重要的傳動部件，因此在交通運輸、冶金、重型機械等領域中得到廣泛應用。基於組件的開發是近幾年發展並日益成熟起來的一種軟體開發新方法，其核心思想是以流水線方式、通過組裝組件生產軟體，從而容易實現軟體開發的工業化。
  4. Owing to the common expression of egf in various organs of mouse, it could be deduced that the role of egf was mainly autocrine and paracrine actions, and its endocrine role was also not excluded owing to its high expression abundance in submaxillary gland

    由於egf在小鼠各器官中表達的普遍性，可以推測egf的作用以旁分泌和自分泌作用為主，但它在頜下博士學位論文「 tnegfalelittin 」嵌合基因構建及有關基因重組表達的研究等器官中的高表達表明其也具有內分泌作用。
  5. Restriction enzymes and dna probes are used to determine the exact sequence, but it is possible to get a rough estimate by analyzing the frequency of recombination between the alleles of linked genes

    雖然可以利用限制性內切酶和dna探針精確的分析出特定序列，但是在分析連鎖基因等位基因間的重組序列時，會出現不準確的估計。
  6. By applying the theory and method of geological anomaly, it is focused on the analysis of the characters of major geological anomalies and their effect of mineralization in daye - jiurui region. the analysis result shows that the basement fault anomalies control a few major large hidden batholiths and their associated orefields ; that the anomalies of cover structure confine most cu - au deposits and fe - cu deposits within their scope ; that the geochemical anomalies and lithofacies - paleogeographical anomalies are major geological factors leading to trataboundness of some cu - au deposits ; that the anomalies of rock operties provide the favorable chemical and physical conditions for the ore - forming process, including the seepage, circulation, convergence and deposit of ore - bearing hydrothermal solution ; that the anomalous ctive stage of mesozoic magmatite is almost consistent with the major mineralization epoch of the fe, cu deposits, and the structure of multi - levels, and the zoning in magmatic system determine the spatial distribution of the mineralization system. then, based on the above analysis, a comprehensive variate, geological combination entropy, is brought forward for reflecting the complex degree of the combination of geological ore - controlling factors. after circumscribing the anomalies of geological combination entropy, the relations between the anomalies and ore deposits are determined

    從地質異常的角度，重點分析了大冶九瑞地區主要地質異常的特徵及其對成礦的影響.由分析可知：基底斷裂異常控制了本區若干重要規模較大的隱伏巖基及伴生礦田，而蓋層構造異常控制銅金礦床和鐵銅礦床的空間位置；地層的地球化學異常和巖相古地理異常是造成一些銅金礦床層控性的主要地質因素；蓋層的巖性異常為含礦熱液的滲流、循環、聚集和礦質沉澱等一系列成礦作用提供了有利的物理和化學條件；中生代巖漿巖的異常活動期也是本區主要的鐵銅成礦期，巖漿巖系統的多層分枝和分帶性結構控制了本區成礦系統的三維空間分佈.在上述分析的基礎上，構置了地質組合熵作為反映控礦地質因素組合系統結構復雜程度的綜合變量，並圈定出組合熵異常，由此確定了綜合地質異常與礦床的關系
  7. In chapter four, on the basis of taking up a great amount of first hand information, the article for the first time puts forward original degree of concentration index numerical value ( 1990 - 2001 ) of china coal industrial market and has engaged more objective analysis and appraisal in relation to so such sensitive problems as influence factor, market structure positioning, especially the orientation of industry policy and enterprise restructure and expansion and has figured out the core content of the article

    同時，在佔有大量一手資料的基礎上，第一次公開推出原創性的中國煤炭產業市場集中度指標數值（ 1990 - 2001年） ，並對其影響因素、市場結構定位，尤其是產業政策取向和企業重組擴張等敏感性問題進行了較為客觀的分析和評價，構造出本論文的核心內容。作為最大的發展中國家，中國歷經10餘年的煤炭市場化實踐，出現了許多值得認真研究並需合理解釋的現實問題。
  8. In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein

    經酶切鑒定及dna序列測定，該基因為雞il - 2基因，其序列與sundick等報道的完全一致。在此基礎上，我們把雞il - 2基因亞克隆到大腸桿菌原核表達載體pproex ~ （ tm ） ht中，構建重組表達質粒並進行確證性序列測定，重組質粒測序結果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原核表達載體pproex ~ （ tm ） ht的目的位點。重組質粒轉化受體菌dh5後用iptg於37進行誘導培養， sds - page和westernblot分析顯示，表達的雞il - 2融合蛋白分子量約為18kda ，表達的融合蛋白經薄層掃描發現目的蛋白表達量約占菌體蛋白的30 。
  9. Aim : to analyze the mechanism, thermadynamic theoretical basis, dynamic mechanism and influencing factors of thermally induced phase separation ( tips ) in order to completely grasp the factors affecting the size, distribution and form of pores, so that the adjusted range of pore can be widened and the preparation of porous membrane can be repeated and controlled. methods : considering from the structural characteristics of tissue engineered materials, the methods of preparing porous membrane using tips technique, the hermadynamic theoretical basis, dynamic mechanism and influencing factors were analyzed, the problems and investigative directions in the future were also analyzed. tips technique is a process of phase separation of polymer homogenous solution under quenching, and it is suitable for diameter and structural form of the micropore materials prepared using tips are closely correlated with the kind and dispensing proportion of polymer attrnuant, polymer concentration and polymer molecular mass, etc. conducted, including determination of polymer - solvent system phase diagram, study of form and appearance of porous membrane of different thickness, study of form and appearance of porous membrane prepared with systems of different x, which is the parameter of polymer - solvent interaction

    目的:分析熱致相分離成膜過程的機理、熱力學理論基礎、動力學機制以及影響因素，以便充分掌握影響孔度大小、分佈、形態的因素，使孔度調控范圍得以拓寬，使多孔膜的制備能重復可控.方法:從組織工程材料結構特點出發，分析熱致相分離聚合物多孔膜的制備方法及該法成膜的熱力學理論基礎、動力學機制以及影響因素.並分析實驗中存在的問題及今後的研究方向.結果:以熱致相分離法可制備聚合物多孔膜.熱致相分離法制備多孔膜是高聚物均相溶液在淬冷條件下發生相分離的過程，它適用於上臨界共溶溫度型聚合物一稀釋劑二元體系.熱致相分離法成膜的過程，可以認為是旋節線機理佔主導地位.熱致相分離法制備的微孔材料，其孔隙率、孔徑大小、結構形態與聚合物稀釋劑的種類、組成配比、聚合物濃度、聚合物分子量等因素密切相關.結論:可採用熱致相分離技術制備多孔膜，通過改變不同的成膜條件可獲得一系列不同孔徑尺寸和孔徑分佈的多孔膜材料.對熱致相分離成膜過程中聚合物-溶劑體系的相圖測定，不同厚度的多孔膜形貌研究，不同x （聚合物-溶劑相互作用參數）體系所制備的多孔膜形貌等需深人研究
  10. First, based on investigation and analysis on real estate background and the actual situation of jiangbei real estate development company, the problems and the foundmental root causes existing in the operation management are found out. secendly, according to the requirements of modern business management theory and modern enterprise system, a business management model with quality management being the core is put forward by comparing and analyzing several management models, and selecting one management model as the major with multiple management models existing. based on this, the author has designed the main frame for the management model, completed organizational structure designing, department function and work position describing, work flow and work process designing, establishment of regulations, and formed the organizational structure of the management model. in addition, the varies important management actions, including human resources management, quality management, financial management, marketing management, information management, are analyzed and studied in order to enrich and improve the content of the management model. finally, the author has also put forward the measures and requirements to ensure the effective operation of the management model

    首先，在調查研究的基礎上，通過對房地產行業背景和江北房地產開發公司現狀的分析，找出公司現行狀態下經營管理中存在的問題和困難，並分析原因，查找根源；其次，根據現代企業管理理論和現代企業制度的要求，通過對幾種較為流行的管理模式的分析比較，選擇以一種管理模式為主，多種管理模式相結合的方法，提出以質量管理為核心的企業管理模式；在此基礎上，對管理模式進行整體性的組織設計，完成組織結構設計、部門職能制定和崗位描述、工作流程及工作程序設計、規章制度體系的建立等工作，構建管理模式的整體框架；另外，針對各項重要的管理活動，如人力資源管理、質量管理、財務管理、營銷管理和信息管理等，分別進行分析研究，以充實和完善管理模式框架的具體內容；最後，為確保管理模式順利、有效地運行，明確提出「完善公司內部治理機制，健全公司外部治理機制」的保障措施和具體實施步驟。
  11. On the basis of collecting and processing many datum and materials. firstly. this paper analyzes main activities and cost constitutions of each stage of the life cycle of a fcs, and lay a foundation for later analysis and evaluation of system lcc. secondly, a basis method and usage range for estimating the system lcc are introduced. a multivariate linear regression model of pcs development cost and cost driven factor is built by use of the parametric method and supplies the base of cost estimation of newly - developed systems. thirdly, combine actual examples and make statistical analysis of lcc of a certain pcs developed by our institute, predict unhappened usage and service cost with grey prediction method, obtain proportion of each constitute to the lcc. forthly, according to actual conditions, use the fuzzy theory to overall evaluate efficacy of the fcs, fifthly, combimng our actual conditions, investigate specific measures of how to implement the life cycle cost management in our institute and put forward a new conception of developing web - based flight control system lcc management information system with pdm as the platform. at last, investigate important factors such as reliability and maintainability that may affect the life cycle cost of the fcs in detail, and put forward specific methods of lowering the life cycle cost of the fcs

    論文在收集和整理大量資料的基礎上，首先深入分析了飛控系統壽命周期各階段的主要活動以及各階段的費用構成，為以後系統壽命周期費用的分析和評價奠定了基礎；其次，介紹了壽命周期費用估算的基本方法和使用范圍，並利用參數法建立了飛控系統研製費用與費用驅動因子的多元線性回歸模型，為新研系統的費用估算提供了依據；第三，結合實例對我所研製的某型飛控系統的壽命周期費用進行統計分析，運用灰色預測方法對未發生的使用及維修費用進行預測，得出了該系統的壽命周期費用以及各組成部分所佔比例；第四，根據實際情況，首次運用模糊理論對飛控系統的系統效能進行了綜合評價，構造了飛控系統系統效能模糊綜合評價模型；第五，結合我所實際，探討了如何在本單位實施加強壽命周期費用管理的具體措施，提出以pdm為平臺，開發基於web的飛控系統lcc管理信息系統的新構思；最後，對影響飛控系統壽命周期費用的重要因素如可靠性和維修性等進行了詳細地研究，提出了降低飛控系統壽命周期費用的具體方法。
  12. 4 wang ea ， rosen vd ， alessandro js ， et al. recombinant human bone morphogenetic protein induces bone formation. proc natl acad sci usa ， 1990 ， 87 : 2220

    5戴毅敏，陳新梅，毛天球，等.基因重組人骨形態蛋白- 2和牛骨形態蛋白異位誘導成骨的比較.實用口腔醫學雜志， 2002 ， 18 （ 4 ） : 326 - 328
  13. Under the horizontal earthquake action and wind force, aim at " the pure frame structure with rectangle columns ", " the pure frame structure with special - shaped columns ", " the frame - truss structure with special - shaped columns " and " frame - shear wall structure with special - shaped columns ", earthquake action analysis was done by the spacial finite element method through the changes structural parameter. analyzing systematically " structure vibration mode ", " vibration period ", " structure lateral rigidity ", " seismic action force ", " seismic response force ", " floor seismic shear force ", " lateral horizontal displacement of structure " and " members internal force ". results indicate : ( 1 ) based on equal area, the special - shaped columns replaced the rectangle columns, the structural lateral rigidity enlarges, the lateral displacement minishes obviously, the earthquake response increase slightly, the biggest increasing amount of frame columns axis - compress ratio is smaller than 0. 08. the whole aseismic performance of structure has improved ; ( 2 ) when the section ' s length and section ' s thickness ratio of special - shaped columns is smaller than 3. 6, the structure benefits to resist seismic action ; ( 3 ) the angle of horizontal seismic action with the whole coordinate is 0 degree, structure earthquake response is bigger, belonging to a control factor of structure aseismic design ; ( 4 ) the frame - truss structure with special - shaped columns and the " a " - brace has the biggest lateral rigidity ; ( 5 ) the frame - shear wall structure with special - shaped columns have bigger lateral rigidity and smaller displacement, members internal force enlarged just rightly, have much superiority of resisting seismic action ; ( 6 ) in the higher seismic fortification criterion region ( 8 degree of seismic fortification intensity ), aseismic disadvantageous building site ( iii type site ), adopting special - shaped columns structure system, should reduce possibly the building ' s self - weight in order to reduce the earthquake response ; ( 7 ) the response spectrum method of computing seismic response and the time - history analysis method have similar analysis result

    西安理工大學碩士學位論文在水平地震作用下，並考慮風荷載組合，分別對「矩形柱純框架結構」 、 「異型柱純框架結構」 、 「異型柱框一析架結構」及「異型柱框-剪結構」等四種結構體系，通過改變結構參數，運用空間有限元方法，進行地震作用計算。系統地分析研究「結構振型」 、 「振動周期」 、 「結構側向剛度」 、 「結構地震反應力」 、 「樓層地震剪力」 、 「結構側向位移」 、 「層間位移角」 ，以及「構件內力」 。結果表明:在等面積原則下，異型柱代換矩形柱后，結構剛度增大，側向位移明顯減小，地震反應力略有增加，框架柱軸壓比最大增幅小於8 % ，結構整體抗震性能有所提高;當異型柱肢長肢厚比小於等於3 . 6時，結構有利於抗震;水平地震作用力與整體坐標夾角為0度時，結構地震作用效應較大，屬結構設計的控制因素之一;異型柱框一析架結構採用「人」字斜撐，側向剛度大於「八」字斜撐和「人一八」字混合斜撐;異型柱框一剪結構側向剛度大、位移小，構件內力增大適中，是一種抗震性能優越的結構體系:在抗震設防烈度較高地區（ 8度）和抗震不利的建築場地（ m類場地） ，採用異型柱結構體系時，應盡可能減輕結構自重，降低地震作用力;分別採用「振型分解反應譜」法和「時程分析」法進行地震作用計算，兩種方法所得結果基本一致。
  14. This paper firstly analyzes the principle of m & a from two aspects of motivation and effects, it also introduces the meaning and types of m & a based on the analyses of the theory of m & a, this paper discusses the m & a process of chinas soes, such as problem of government behavior orientation, problem of capital evaluation of enterprises, problem of laws and regulations, problem of obstacles from stock market, problem of social security, problem of blind m & a, and so on. in order to solve mese problems, is necessary to clarify the behavior criterion of government in the process of m & a build up normative capital market and finance tools, establish sound social security system, establish and consummate the relevant law system, choose the suitable type of m & a, establish and consummate the capital evaluation system and surveillant organizations for property rights trades

    本文首先從企業並購的動因和並購的效應兩方面分析了企業並購的基本原理。在此基礎上總結了我國企業並購的發展歷程、特點及主要模式，分析了我國國有企業並購的內在動因及並購對生產要素重組的作用，最後從政府行為定位、資產評估、法律問題、證券市場的障礙等方面分析了企業並購中的盲目行為。最後論文認為規范政府的行為、建立規范的市場和金融工具、建立完善的社會保障體系、健全和完善相關的經濟和金融法規、建立健全資產評估機構及產權交易監督機構，是規范並購行為的必然選擇。
  15. Hegf gene with his - tag at the end, which was derived from pet22 - egf, was in - frame fused to the carboxy - terminal of polyhedrin ( ph ) gene, which included the amino - terminal 116aa coding region. the polyhedrin - egf fusion gene ( named ph - egf ) was then cut out with ecorv and ecori, and was cloned between ecorv and ecori sites of pbacpak. 8 ( the result plasmid was named pbacph - egf and the ph - egf fusing gene was right under the control of ph promoter ). the pbacph - egf structure was verified with restriction enzymes digestion and pcr

    從pet22 - egf質粒中分離出末端帶his - tag的egf基因，對位融合於多角體蛋白n端116個氨基酸基因序列的下游（命名為ph - egf ） ，並在兩段基因間設計了凝血酶xa蛋白酶切位點，經過酶切、測序等鑒定正確后，克隆至pbacpak8中，使ph - egf融合基因置於多角體蛋白（ polyhedrin ， ph ）基因啟動子控制之下，構建成重組轉移載體pbacph - egf 。
  16. This thesis makes a systematic and detailed study on technical economic characteristics of petrochemical industry, supply and marketing mannel, logistics method and the business resources, and then devises a strategy on the third party ' s storage providers. in this thesis, storage management strategy is discussed, including infrastructure and resource distribution, business process and organization redesign, information system construction, manufacture management and human resources management. afterwards the marketing strategy of the third party ' s storage providers, cost management strategy and consumer relationship management is also designed

    本篇論文在分析了中國石化行業企業改制重組、遼陽地區石化行業技術經濟特點、石化企業供銷渠道和物流模式、以及遼化物資供應公司經營資源等因素后，制定了公司策略，其中包括基礎設施與資源配置、業務流程優化與組織結構再造、信息系統建設、企業文化建設和人力資源管理等方面，並為該企業作出了第三方倉儲專業服務商的戰略定位。
  17. To study the biological function of sh2a gene, we constructed its recombinant expression vec - tor, and investigated its function by cell transfection, kinase assay, subcellular localization and expression analysis. materials and methods 1

    我們構建了sh _ 2a基因的真核重組表達載體，通過細胞轉染、激酶活性檢測、亞細胞定位、表達分析和流式細胞儀等方法對其功能進行了初步的研究。
  18. The first chapter, general discussion of a company ' s contraction strategy, analyzing the basic concept, origination and development of asset reorganization and concept and motive of company contraction. the second chapter, the relation ship between company ' s contraction strategy and m & a, analyzing misunderstanding of m & a and relation ship between company ' s contraction and m & a. the third chapter, strategy analysis of company ' s contraction, discussing company ' s contraction and multiple operation, and company ' s contraction and company ' s competition strategy. the fourth chapter, method divestment of company ' s contraction, analyzing some firms adopted at domestic and abroad, including divestiture, spin - off, equity crave - out, stock repurchase, targeted stock and voluntary liquidations

    論文的結構體系為：第一章公司緊縮戰略概述，分析了資產重組基武漢理工大學碩士學位論文本概念、起源和發展和公司緊縮的概念以及公司緊縮的動因；第二章企業並購與緊縮的關系分析了企業並購的誤區和公司緊縮與並購的關系；第三章公司緊縮的戰略分析，討論了公司緊縮與企業價值鏈分析和企業核心能力理論與公司緊縮；分析了多元化經營與公司緊縮和公司竟爭戰略與公司緊縮戰略：第四章公司緊縮戰略的路徑設計，分析了國內外採用的幾種方式：資產剝離、分拆上市、公司分立、定向股、股份回購等。
  19. But, in our country, culture integration in the process of m & a is not satisfying at present, which is disadvantageous for successful expansion and corporate development. this paper points out the factors which bring out lag and failure of culture integration. based on the analyses, it attempts to provide a set of thoughts, mainly involved with cultural intention of m & a, principle and orientation, guiding mode and countermeasures for effective cultural integration

    本文首先對並購重組中的企業文化進行了分析，強調了進行企業文化整合的必要性和重要意義；然後，通過對我國企業並購重組目標和文化任務的特殊性及其文化整合現狀的基本分析和判斷，指出了當前我國企業並購重組文化整合普遍遲滯與失敗的原因；最後，針對我國並購重組企業文化整合中存在的問題，從企業並購重組出發點的文化定位、企業文化整合的原則取向、指導模式以及增強文化整合實效性的對策等方面提出了解決問題的思路。
  20. Therefore, a - amylase has been used widely in many industrial fields, such as glucose production, beer brewing, fermentation trade, textile industry, and so on. the study on a - amylase is one of the most active fields in enzyme industrial fields. with the development of biotechnology, more and more scientists and researchers attempt to use dna shuffling technology to breed, screen and even " create " new a - amylase genes with higher activity and other new characters

    設計引物時，上下游引物5 』端分別添加了kpn和bamh酶切位點，克隆得到的基因片段和質粒載體都用kpn和bamh進行雙酶切后，進行酶連、轉化、篩選得到陽性重組子，經過藍白斑驗證、單酶切驗證、雙酶切驗證、 pcr驗證等一系列的驗證后進行測序。
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