終止密碼子 的英文怎麼說

中文拼音 [zhōngzhǐmìmǎzi]
終止密碼子 英文
stocodons
  • 終 : Ⅰ名詞1 （最後; 末了） end; ending; finish 2 （指人死） death; end 3 （姓氏） a surname Ⅱ形容詞（...
  • 止 : Ⅰ動詞1. （停止; 攔阻） stop; cut out 2. （截止） close; end Ⅱ副詞（僅; 只） only; just Ⅲ名詞（姓氏） a surname
  • 密 : Ⅰ名詞1 （秘密） secret 2 [紡織] （密度） density 3 （姓氏） a surname Ⅱ形容詞1 （距離近; 空隙小）...
  • 碼 : Ⅰ名詞（表示數目的符號或用具） a sign or object indicating number; code Ⅱ量詞1 （指一件事或一類的...
  • 子 : 子Ⅰ名詞1 （兒子） son 2 （人的通稱） person 3 （古代特指有學問的男人） ancient title of respect f...
  • 終止 : 1 （結束） stop; end; suspend 2 （停止） termination; annulment; abrogation 3 [音樂] cadence; 終...
  • 碼子 : 1. （數目符號） numeral 2. （籌碼） counter; chip
  1. Chain termination codon

    鏈終止密碼子
  2. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  3. The start reading framae and stop codons, base composition in protein - coding genes and the codon usage of amino acids in scolopendra multilane were compared with the three other myriapods

    本研究在蛋白質編碼基因起始閱讀框和終止密碼子、蛋白質編碼區的堿基組中文摘要成、氨基酸及密碼子的利用等方面把少棘蜈蚣與另三種多足類進行了比較。
  4. Sv40 polya signal and two loxp sites was added consecutively after the termination codon taa, and then a gfp gene was inserted between the two loxp sites

    在三個終止密碼子taa之後，連上sv40polya 。 sv40polya之後，又連上兩個loxp位點。在兩個loxp位點，插入標記基因gfp 。
  5. A late baculoviral transcription initiation motif ataag was found 65 nt upstream of the putative translational start site and a polyadenylation signal aataaa was identified 14 nt downstream of the taa stop codon

    在其起始密碼子上游- 65nt發現桿狀病毒晚期啟動子轉錄起始信號ataag ，在其終止密碼子下游- 14nt發現polya加尾信號aataaa 。
  6. Pcr method was used to identify candidate ms 188. sequence analysis indicated that a point mutation occurred in the second exon of the atmyb103 gene in male sterile mutant with caa ( gln ) replaced by a stop codon taa

    利用pcr的方法從突變體中擴增atmyb103基因並進行序列分析，結果表明突變體中atmyb103基因第二個外顯子上發生了點突變，由原來編碼谷氨酰胺的caa密碼子突變為終止密碼子taa 。
  7. Analysis of the sequence variation of cytochrome b gene indicated that there is no evidence of insertions or deletions, i. e., they are all of identical length of 1143 bp in all the sequences of cytochrome b gene. further, the sequences can be fully translated into amino acid using chicken mitochondrial codon without nonsense mutations or intervening stop codons. the 1143 bp cytochrome b alignment contained 416 variable sites, of which 306 were parsimony informative sites with the strongest variable in third codon positions and less variable in first and second codon positions

    細胞色素b基因序列變異分析表明： 1 ）雁形目鳥類細胞色素b基因全序列長度一致，無插入和缺失：對照雞線粒體密碼子系統全序列能全部翻譯成氨基酸序列，無無義突變，全序列內部無終止密碼子； 2 ）序列比對后1143加，含416個核著酸變異位點， 306個簡約信息位點，其中處於密碼子第三位的變異最大，第一位和第二位堿基的變異相對較小。
  8. Compared hasnpv helicase with the helicases of autographa colifornica mnpv ( acmnpv ), ( bombyx bori npv ( bmnpv ), lymantria dispar mnpv ( ldmnpv ) spodoptera exigue mnpv ( semnpv ), orgyia pseudotsugata mnpv ( opmnpv ), and xestia c - nigrum granuloviurs ( xcgv ), only 5 motifs ( i, la, ii, iii, iv ) were found conserved in baculovirus

    其在終止密碼子下游第12位有? polya終止信號aataaa 。 hasnpv解螺旋酶與其它5種桿狀病毒解螺旋酶相比，有5個基元序列（ 、 a 、 、 、 ）保守，另外兩個（和）完全不保守。
  9. Using 3 ' race the transcription stop site mapped 27nt downstream of the putative translation stop codon

    3 』 race分析結果表明轉錄的終止位點在翻譯終止密碼子下游的27個堿基處。
  10. Conclusion we have constructed the expression plasmid pbv220 - hpf4 successfully. 3 ' - utr of h pf4 c dna was deleted and tag was mutated to taataa by pcr. after sds - page and densitometric scan analysis, the expression level of r hpf4 is 25 - 30 %

    結論本研究運用pcr定點突變技術，完全去除了hpf4cdna基因3 」端utrat富含區：改用大腸桿菌強串聯終止密碼子taaaataa ，成功構建高效表達克隆pbv220 rhpp4 。
  11. The sequence encodes an open reading frame of 518 amino acids. there is a transit peptide of 74 amino acids in the n terminal of the putative amino acids sequence coded by the cdna

    該序列長為1758hp ，起始密碼子位於84 86hp ，終止密碼子為1658 166fop ，開放閱讀框長為1557hp ，編碼58個氨基酸序列，其中n末端含有74個氨基酸的轉運肽。
  12. In this research two full - length cdnas have been cloned by a combination of rt - pcr and 3 " - is1 - race with synthesized degenerate primers from young leaves of vicia faba l., pichia methanolica high - level expression systems of the genes have been constructed, and the milligram expressed protein was purified using probond resin purification system, which may result in further identification of the function of the aba binding protein. the full - length cdna of abp370 fragment is 3449 bp long and has an open reading frame of 2304 bp encoding 768 amino acids with 876 bp long 5 ' - utr, 369 bp long 3 ' - utr and poly ( a ) tail. the full - length cdna of abp640 fragment is 1012 bp long and has an open reading frame of 780 bp encoding 260 amino acids with 88 bp long 5 ' - utr, 144 bp long 3 " - utr and poly ( a ) tail

    3 - 5 - race擴增片段序列分析結果表明， abp370擴增片段的全長cdna為3449核苷酸，其中5非翻譯區為876個核苷酸， 3非翻譯區為369個核苷酸並末端帶poly （ a ）尾巴，從起始密碼子atg至終止密碼子tga ，含有一個編碼768個氨基酸殘基的開放閱讀框架（ 2304bp ） ； abp640擴增片段的全長cdna為1012核苷酸，其中5非翻譯區為88個核苷酸， 3非翻譯區為144個核苷酸並末端帶poly （ a ）尾巴，從起始密碼子atg至終止密碼子taa ，含有一個編碼260個氨基酸殘基的開放閱讀框架（ 780bp ） 。
  13. The cdna is 2 149 bp long with an open reading frame of 1 697 bp, which encodes a polypeptide of 565 amino acids, preprotein of 62. 3 kda ; 5 " untranslated regions contains 59nt, 3 " untranslated regions contains 391nt, a poly ( a ) tail signal and long poly ( a ) tail

    該cdna全長2149bp ，包含一個1697bp的開放閱讀框架，編碼565個氨基酸。在起始密碼子上游有一個由59個堿基組成的5 』非編碼區；在終止密碼子下游有一個由391個堿基組成的3 』非編碼區，包括4個分解信號、 1個加尾信號和1個長度為17個腺苷酸的poly （ a ）尾。
  14. After sequencing of 84 cdna clones and removing redundant cdnas, we obtained 36 cold - regulated unique cdna clones. 12 cdna clones were expected to be novel genes, because no sequence homology with any known sequences was found in genbank databases

    全長基因ej175共有603bp ，對其可讀閱讀框架進行分析，從57 - 515位核苷酸的一段序列，包含了起始密碼子和終止密碼子，編碼152個氨基酸的多肽。
  15. The fact results in the conclusion that the bases related to the stop codon tga are strictly constrained to be used

    由此得出結論：終止密碼子對蛋白質編碼區堿基的使用具有限製作用。
  16. Three codons, uaa, uag, and uga serve as punctuation codons and are not translated into amino acids

    Uga這三個密碼子不翻譯成氨基酸，起終止密碼子作用。
  17. The statistical results show that the bases and dinucleotides with low contents are just associated with those in the stop codon tga

    對單核苷酸和雙核苷酸的使用進行統計分析，結果表明與終止密碼子對應的組分的含量都很低。
  18. The tk gene contained an open reading frame of 957 bp encoding a 318 aa protein. upstream of the tk orf, three putative gc boxes are located at positions - 22, - 166 and - 199. a potential poly a signal begins 110 nucleotides downstream from the termination codon at position 1306

    在tkorf上游- 22 、 - 166 、 - 199位存在3個gc框樣序列，在終止密碼子下游第110個核苷酸處的1306位存在有多聚腺苷加尾信號。
  19. A polypeptide with sequence of qkvdssggggs was designed to be a linker between c terminal of penicillin g acylase and n terminal of the coat protein. the ribosome binding site ( rbs sequence ) of psurfscript is also replaced by rbs sequence originating from bacillus subtilis. it was demonstrated that constructed phagemid can still express penicillin g acylase

    將包含信號肽和琥珀終止密碼子uag （ amber ）的完整巨大芽孢桿菌青霉素g酰化酶基因克隆到噬菌粒psurfscript ，通過引入的11肽連接青霉素g酰化酶的c末端與噬菌體外殼蛋白gp3的n末端。
  20. Vp2 and vp3. the genes, encoding them, were located in a same orf, and they possess of common stop codons. furthermore, amino acid sequence of vpl contained the fu1l sequence of vp2 and vp3, so vp1 gene become interest gene. in this research

    Gpv的三種結構蛋白vp1 、 vp2 、 vp3位於同一開放閱讀框內，共用同一終止密碼子， vp1包含了組成vp2 、 vp3的所有氨基酸，因其具有這一特殊性，成為本研究的目的基因。
分享友人
分享到 Line

分享到臉書