芥子素 的英文怎麼說
中文拼音 [jièzisù]
芥子素
英文
myroxin-
Our previous studies showed existence of apoplast cam in the plant cell and cam had many extracellular functions. so it supposed cam may be one of important extracellular polypeptides and trigger the intracellular signal transduction by binding the receptor. in this study, radiolabelled ligand is used to investigate the binding characteristic of cam and a. thaliana protoplasts. and chemical crosslinking is employed to explore binding proteins in the membrane. at first, ( 35 ) ~ s - cam was produced using ( 35 ) ~ s - labeled amino acid mixture in e. coli. sds - page and autoradiograph indicated high - purified, high - specific radioactivity ( 35 ) ~ s - cam was obtained. electrophoresis of ( 35 ) ~ s - cam is the same as that of unlabeled cam with ca ~ ( 2 + ) or egta ; a quatitive of protoplasts was prepared by enzymolysis
首先，用~ （ 35 ） s標記的氨基酸混合物喂養工程菌成功地制備了~ （ 35 ） s標記的擬南芥鈣調素（ ~ （ 35 ） s - cam ） ， ~ （ 35 ） s - cam純度高、放射活度高、 ca ~ （ 2 + ）與egta存在時的電泳行為與未標記cam相同，可作為一種高靈敏性的探針用於進行受體學分析實驗；用擬南芥種子誘導愈傷，通過酶解制備了大量原生質體。Dark green leafy vegetables such as broccoli, spinach, collards, turnip greens and yellow vegetables and fruits such as apricots are rich in biochemical substances that protect vision from one of the major factors in age - related macular degeneration, say richard bone, professor of physics, and john landrum, associate professor of chemistry, in the latest issue of the journal methods in enzymology
物理教授理察柏恩及化學副教授約翰蘭卓姆最近在酵素學方法期刊上發表指出，深綠色葉子的蔬菜，像花椰菜菠菜芥藍大頭菜，還有黃色的蔬菜和水果，像是杏桃，都含有一些豐富的生化物質，這些生化物質是對抗中老年視斑性退化的一個主要因素。Using the young seedlings of wild type ( wt ) and four mutants ( phya, phyb, phyaphyb and hy4 ) of arabidopsis thaliana as plant materials, the effects of light quality and kinetin ( kt ) on some photomorphogenesis responses such as cotyledon enlargement, hypocotyl growth inhibition, accumulation of chlorophyll and anthocyanin were investigated in the present study. photoreceptor functions and interaction of light and kt in the responses was examined and discussed
本文以擬南芥（ arabidopsisthaliana ）野生型（ wt ）和四種光突變體phya 、 phyb 、 phyaphyb和砂hy4為材料研究不同光質與細胞分裂素（ kt ）對幼苗子葉擴大、下胚軸伸長、葉綠素和花色素苷積累的影響，分析光受體參與的反應以及探討光與kt在調節各種反應中的相互關系。Iraq ' s procurement efforts include equipment that can filter and separate micro - organisms and toxins involved in biological weapons, equipment that can be used to concentrate the agent, growth media that can be used to continue producing anthrax and botulinum toxin, sterilization equipment for laboratories, glass - lined reactors and specialty pumps that can handle corrosive chemical weapons agents and precursors, large amounts of vinyl chloride, a precursor for nerve and blister agents, and other chemicals such as sodium sulfide, an important mustard agent precursor
伊拉克采購的設備包括可過濾和分離生化武器中微生物和毒素的設備；可用於為炭疽病毒和肉毒（桿）菌病毒集中藥劑和生長媒體的設備；實驗室殺菌設備；可處理腐蝕性化學武器藥劑、前體、乙烯基氯化物（一種神經和水泡藥劑）及其他化學藥劑（如鈉硫化物，芥子氣藥劑的前體）的玻璃線紋反應堆和專業水泵。The activity of apx was also decresed after sa and vd - toxin treatment. the activity of cat was decreasded more obviously. in the vd - toxin infected arabidopsis, no also restrained the activity of cat and decreased the level of cat3 mrna. the results suggested that h2o2 acted as a signal to involve the defense reactions which is induced by sa and no in arabidopsis
No在毒素與擬南芥的互作過程中也表現出對cat的抑制作用，結果表明， h _ 2o _ 2可能作為信號分子參與了sa和no調控的擬南芥的防衛反應， sa和no或許在轉錄和蛋白水平上通過對cat的抑制作用而促進h _ 2o _ 2的積累。The resulting plasmid, named prok - sod2, was mobilized to agrobacterium tumefaciens strain gv3101 used for plant transformation. the yeast sod2 gene was introduced into arabidopsis thaliana ( ecotype landsberg erecta ) by agrobaterium tumefaciens - mediated transformation with floral - dipping method under the control of camv 35s promoter. transformants were selected for their ability to grow on medium containing kanamycin ( 30mg / l ), several homozygous lines that were all tolerant to kanamycin were selected and used for further molecular and physiological determination
本實驗將sod2基因構建到植物表達載體prok中，導入農桿菌后，進行植物遺傳轉化，實現其在擬南芥中過量表達，在含30mg l的卡那黴素的培養基上篩選獲得純合轉基因株系，自交一代獲得足夠的純和轉基因種子后，對其進行了分子生物學的驗證及生理指標的檢驗。Huangyal4 was complete nucleotide sequence of 1 854 bp with a nucleotide orf ( 1575 bp ), which encoded a protein consisting of 524 aa with molecular weight of 62. 2 kda and pi of 8. 96. strongly basic ( + ) amino acids, strongly acidic ( - ) amino acids, hydrophobic amino acids and polar amino acids of the protein were 13. 74 %, 11. 64 %, 36. 45 % and 22. 70 % respectively, and predicted secondary structure of the protein revealed many conserved domains such as n - glycosylation site, protein kinase c phosphorylation site, casein kinase ii phosphorylation site, n - myristoylation site, camp - and cgmp - dependent protein kinase phosphorylation site, tyrosine kinase phosphorylation site and a cytochrome p450 cysteine heme - iron ligand signature which was typical of cytochrome p450. a - helix and b - sheet of the protein is 47. 7 %, 45. 0 % respectively
Huangya14 ）為材料分離克隆到一個細胞色素p450基因，命名為bccyp86mf5 ， cdna全長1854bp ，含1575bp的完整開放閱讀框，編碼524個氨基酸，其編碼蛋白質的分子量為61 . 2kda 、等電點為8 . 96 ；堿性氨基酸、酸性氨基酸、疏水氨基酸和極性氨基酸分別占總氨基酸的13 . 74 、 11 . 64 、 36 . 45和22 . 70 ；二級結構預測包括n -糖基化位點、依賴于camp和cgmp的蛋白激酶磷酸化位點、蛋白激酶c磷酸化位點、酪蛋白激酶磷酸化位點、酪氨基酸激酶磷酸化位點、 n -豆蔻酰化位點和細胞色素p450的典型區域，半胱氨酸亞鐵血紅素配體信號區等， -螺旋和-折疊分別佔47 . 7 、 45 . 0 ；與bccyp86mf1基因的氨基酸序列同源性達到95 . 2 ，與擬南芥cyp86c4的達到85 . 9 。These results revealed that there may be a complicated interaction among the three signal moleculars no, sa and h2o2 in the responses of arabidopsis to toxin of verticillium dahliae. the interaction played important function in the defence reactions of arabidopsis
綜上所述，在大麗輪枝菌毒素與擬南芥幼苗的互作反應中，信號分子no 、 sa和h _ 2o _ 2之間可能存在復雜的相互關系，這種相關性在誘導擬南芥幼苗的防衛反應中起重要的調控作用。6. transformation system of mustard a serials of kanamycin concentration was added to optimum medium to test the explants resistance capacity of two kinds of mustard. the transformation procedures described were derived from numerous regeneration and trasformation designed to test factors that might affect shoot regeneration, which including length of co - cultivation. those producing the best result parameters were described as below : after the mustard explants were precultured on regeneration medium for 2 days. they were inoculated with agrobacterium for 20 minutes. inoculated explants were co - cultivated for 4 days and in shadow at first 2 days. then transferred to the same medium plus 30 mg / l kanamycin and 500mg / l garb. all of them were transferred to fresh medium every 2 weeks. the kan - resistant plants were regenerated
芥菜外植體高頻遺傳轉化體系的建立在最適培養基上試驗了兩類芥菜的三種外植體對卡那黴素的敏感性、預培養天數、浸菌時間等因素的影響，建立了芥菜高頻轉基因再生體系：取生長4天的芥菜子葉、下胚軸和25天的葉片在分化培養基上（ ms + ba3 . 0mg / l + naa0 . 1mg / l ）預培養2 - 3天後，投入農桿菌菌液中浸染20分鐘，在分化培養基上暗培養2天，正常條件下培養2天後，轉入抗性培養基（ ms ba3In this paper, we summarized the research advances on the gene cloning, sequence and structure analysis, function identification, expression and regulation of the ion transporting proteins and focused on their functions in the process of absorption, distribution and translocation of nitrogen, phosphorus, potassium and other nutrients in arabidopsis thaliana
文中綜述了擬南芥中養分離子轉運蛋白在基因克隆、序列與結構分析、功能鑒定、表達與調控方面的研究進展，其中著重討論了這些轉運蛋白在氮、磷和鉀等營養元素吸收、運輸、分配中的作用。The level of cytosolic free calcium in gp mutants is not different. adding exogenous purified cam to the cultural medium increased the pollen germination, tube growth, and cytosolic free calcium concertration in both wild type and ga mutant. however, the former was more sensitive to exogenous cam than the latter
外源鈣調素（花椰菜和擬南芥11亞型， 10一7m ）處理能夠提高野生型及gpol缺失突變體中的游離鈣離子水平，但對野生型花粉細胞游離鈣離子水平的激活效應高於gpal缺失突變體。分享友人