蟲綠蛋白 的英文怎麼說
中文拼音 [chónglǚdànbái]
蟲綠蛋白
英文
insectoverdins-
To study the localization of the peptide, we also generated recombinant viruses containing the ctl - egfp fusion gene, following observation of infected larvae by such viruses will provide insight into the function of baculoviral conotoxinlike genes
為了研究毒素在蟲體中的定位,構建了類蝸牛毒素和增強的綠熒光蛋白融合的重組病毒racbacctlgfp2和rhabacctlgfp ,為下一步研究奠定了基礎。2. expression of gfp gene in b. thuringiensis strain green fluorescent protein gene is a very useful report gene which could be detected easily. it can be used in the detection of b. thuringiensis environmental release and study of the insecticidal mechanism
綠色熒光蛋白基因在蘇雲金芽胞桿菌中的表達綠色熒光蛋白基因是一種容易檢測的報告基因,可用於蘇雲金芽胞桿菌制劑施用后的環境監測,殺蟲機理等方面的研究。The ha 122 protein, when fused to gfp was observed in the nuclei of h. armigera cells, but only in conjunction with wild type hasnpv infection
將ha122與綠色熒光蛋白( gfp )基因融合,在昆蟲細胞中瞬時表達。當有病毒感染同時發生時, gfp信號定位在核內。3 antimicrobiol substance and protein in the hemolymph of nymphae induced by m. anisopliae no antimicrobiol substance was detected in the hemolymph of nymphae by different kinds of micropathogens after injection with m. anisopliae, which meant that the fungus could not induce p. americanna to produce antimicrobiol substance. injection of fungal spores caused the decline of protein concentration ( pc ) at the begaining, but after that the pc increased rapidly
3綠僵菌孢子對蜚蠊血淋巴中抗菌物質及蛋白質的誘導注射綠僵菌后的不同時期提取蜚蠊血淋巴,以多種不同類型的植物病原菌、昆蟲病原菌及腐生菌作為檢測菌,均未檢測出蜚蠊血淋巴中有抗菌物質存在,即綠僵菌不能誘導蜚蠊產生抗菌物質。In order to get the soluble recombinant eo protein and inspect the protein expression status convinently, the egfp and eo gene were ligated into baculovirus transfer vector. with the co - transfecting sf9 cells of baculovirus recombinant transfer vector and linearized viral dna, and plaque purification in the posttransfection procedure, the pure recombinant baculovirus were harvested, which infected the sf9 cells for amplifying to generate a p - l stock. in the meantime, the fluorescence microscopy detection indicated expressed egfp protein to confirm the heterogenous protein expression of recombinant baculovirus. the pi - stock from a pure plaque was used to generate a high liter p - 2 stock, which was determined in liter as 1. 14 107pfu / ml by performing a plaque assay. when a volume of p - 2 stock infected the sf9 cells with moi 5 - 10 for expression, the strong fluorescence was obeserved on the day 3 of postinfection
此外,為了得到可溶性重組eo蛋白並便於觀察重組蛋白的表達情況,我們將egfp基因與eo基因相連插入昆蟲桿狀病毒轉移載體中,與線性桿狀病毒dna共轉染sf9細胞后通過噬斑純化得到純的重組桿狀病毒,將其感染sf9細胞制備p1種子液,同時用熒光顯微鏡觀察綠色熒光蛋白的表達情況剔除表達效果差的重組桿狀病毒。再用p1種子液感染sf9細胞制備高效價的p2種子液。通過病毒液的梯度稀釋和噬斑測定,確定p2種子液的病毒滴度達1 . 14 10 ~ 7pfu ml 。分享友人