雜交細胞系 的英文怎麼說
中文拼音 [zájiāoxìbāoxì]
雜交細胞系
英文
hybrid cell line- 雜 : Ⅰ形容詞(多種多樣的; 混雜的) miscellaneous; varied; sundry; mixed Ⅱ動詞(混合在一起; 攙雜) mix; blend; mingle
- 交 : Ⅰ動詞1 (把事物轉移給有關方面) hand over; give up; deliver 2 (到某一時辰或季節) reach (a cert...
- 細 : 形容詞1 (條狀物橫剖面小) thin; slender 2 (顆粒小) in small particles; fine 3 (音量小) thin ...
- 胞 : Ⅰ名詞1 (胞衣) afterbirth2 (同一個國家或民族的人) fellow countryman; compatriot Ⅱ形容詞(同胞...
- 系 : 系動詞(打結; 扣) tie; fasten; do up; button up
- 雜交 : [生物學] hybridize; cross; hybridization; cross breeding
- 細胞 : cell; sytes; bioplast; cella; [口語] gene; [生物學] cellule; cellule cellulli cellulo ; cello ; k...
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Even so, by truncating hbv pres gene, we finally obtained some useful " " bailors ", either nontoxic or self - activating, and used them to fish dna fragments of hbv pres interacting protein ( s ) from an ad vector constructed human embryonic cdna library
我們通過第回軍巨大學碩士學位論文對pres基因分段截短的方法,獲得了對酵母細胞即無毒性作用,又沒有自激活作用的「誘餌」 ,通過它在酵母雙雜交系統中篩選構建於ad載體的人胎肝。Serious cross reation existed between v. albo - atrum and mv2, mv3, mv4. the other pathogen isolates v31 and v32 also had cross reactions, but the reaction was not serious. because limited number of pathogen isolates were selected, it could not prove that the selected immunogen was widely presentative, more pathogens isolates should be tested to verify the acquired hybridomas cells
5株單抗雜交瘤細胞中沒有一株具有種或屬的特異性,其中mv2在棉花黃萎病菌若干菌系間的檢測表明其能夠區分不同的致病類型; mv1和mv4組合檢測的結果基本上能將棉花大麗輪枝菌鑒定到種;黑白輪枝菌與mv2 , mv3 , mv4的交叉反應比較強烈,其他菌株v3 , v32有個別的交叉反應,但不強烈The results showed that all six tfs displayed no autoluminescence in this two - hybrid system, and one of them, the atf3 displayed a strong interaction with restin. to verify the interaction of restin and atf3, a series of truncated fragments of atf3 were inserted into pact to be used in the further study
三、為了進一步驗證atf3與restin的相互作用,對atf3進行截斷並構建了一系列atf3的截短片段的雙雜交質粒(仍然構建於pact載體中) ,再次運用細胞雙雜交進行相互作用的探索。Meq protein, highly expressed in the insect cell line sf9 by the baculovirus vector was immunized into balb / c mice and the immunized spleen cells were collected and fused with the tumor cell line sp2 / 0 via peg - 1000 in vitro. the hybridoma cells were cloned and screened for the ability of anti - meq mcab secretion by fa with the mdv ga infected chicken embryo fibroblast ( cef )
利用通過桿狀病毒載體在昆蟲細胞系sfg上高度表達的meq蛋白產物免疫balb / c小鼠,然後收獲其免疫脾細胞並與腫瘤細胞系spz / 0通過peg于體外融合;獲得的雜交瘤細胞被克隆並通過與mdv感染的雞胚成纖維細胞( cef )做免疫熒光試驗( fa ) ,進行其分泌抗meq單克隆抗體( mcab )能力的篩選。Quality of oil determined by the constituents and proportion of fatly acid components, and the understanding of heredity of fatty acid components is of importance to breeding for good quality soybean varieties
摘要利用5個大豆品種配製20個雜交組合,採用廣義種子遺傳模型分析了大豆脂肪酸組分的胚、細胞質和母體植株等3套遺傳體系的基因主效應和基因型環境效應。Establishment of embryogenic callus suspension culture system in liriodendron hybrids
雜交鵝掌楸胚性細胞懸浮系的建立Unicolor and lilium asiatic hybrids or cultivars in lilium asiatic hybrids were researched with their parents by karyotype, soluble protein, esterase isoenzyme and peroxidase isoenzyme. the results provided identification markers of cytology and biochemistry for hybridization at the early stage in lily breeding programs. the cluster analysis according to similarity coefficient of soluble protein and peroxidase showed that " yellow " and " omega " have the most closest relation in lilium asiatic hybrids
本研究對百合屬幾個植物的親緣關系進行了可溶性蛋白質、過氧化物酶同工酶分析,同時對亞洲百合雜交系內雜交及其與原種系間的雜交後代進行了核型、可溶性蛋白質、過氧化物酶和酯酶同工酶分析,以期為百合屬植物親緣關系分析提供生化依據,以及為雜交後代的早期鑒定提供細胞學、生化水平的檢測指標。Effect of cytoplasmic male sterile genes on agronomic and biochemical characters in cms lines and their f1 hybrids in pepper
辣椒細胞質雄性不育基因對不育系及雜交一代農藝性狀和生化特性的影響The effects of glp - 1 ( 7 - 36 ) nh2 on insulin secretion glp - 1 ( 7 - 36 ) nh2 with the concentration of 2. 5nmol / l, 5. 0nmol / l, l0. 0nmol / l, 20. 0nmol / l, 40. 0nmol / l respectively were added to the medium as different experimental groups, 24 hours later, insulin amount are 68. 76 ? 1. 71 72. 30 ? 3. 13 104. 16 ? 5. 57 110. 98 ?. 29 111. 58 ? 0. 65miu / l respectively, and the insulin account is 55. 53 ?. 63miu / lin the control group. there was no significant difference between the groups with 2. 5nmol / land 5. 0nmol / l glp - 1 ( 7 - 36 ) nh2 respectively ; and there was not significant difference among the groups with lo. onmol / l, 20. 0nmol / l and 40. 0nmol / l glp - 1 ( 7 - 36 ) nh2 respectively. but the difference is significant between experimental groups and control group ( p < 0. 05 ). the data show that with the rising concentration of glp - 1 ( 7 - 36 ) nh2, there is an increasing amount of insulin
對照組培養液中不含g廿一1 ( 7一36 ) nhz ,實驗組培養液中含有20nmol / lglp一1 ( 7一36 ) nhz ,培養24h后,用0 . 25 %胰蛋白酶消化胰島分散細胞,塗片后利用針對胰島素mrna的寡核甘酸探針進行細胞原位雜交, dab顯色,高清晰度病理圖文分析系統( highpathologiealimageanalysissystem , hp認s )對細胞著色的平均光密度( mean即tiealdensity , mod )量化分析,觀察實驗組和對照組胰島素mrna的表達情況。The results showed that the compatibility indexes were obviously higher when the male sterile lines were used as the female parent than when their respective maintainer counterparts were used as the female parent, indicating the male sterile cytoplasmic effects on interspecific cross incompatibilities in cruciferae
結果表明:三種類型的不育系均有一致的趨向,即用不育系和保持系與同一親本雜交的親和指數,不育系作母本的明顯高於以保持系作母本的組合,表明十字花科種間雜交親和性具有雄性不育細胞質遺傳效應。Cellular organelles of eukaryotes have a distinctive spatial distribution and communicate through an elaborate system of vesicular - tubular transport
真核細胞中細胞器在空間上分佈不同,各細胞器之間通過復雜的囊泡運輸和微管系統進行物質交換和信息傳遞。In y2h, rapgap from c. elegans was used as bait to screen c. elegans cdna library. after - lth screen, 63 colonies were checked
在酵母雙雜交系統中,將ppc97 rpg 」與ppc86七dna文庫共轉化入酵母菌y190細胞,經過一lth篩選后,有63個菌落擬為陽性菌落。Metallothionein - 3, previously named as growth inhibitory factor, is the unique member of the metallothionein family that demonstrates bioactivity in vitro. to test if mt - 3 dimerizes or polymerizes itself
為了驗證其在細胞內是否形成二聚或多聚體,構建酵母雙雜交系統用於檢測。In the present study, the express library of monoclonal anti - sp18 scfv ( single chain fragment variable ) gene is constructed and selected for further study of sp18 antigen on mammalian fertilization and embryogenesis. total rna were firstly isolated from these growing hybridoma cells which secretes monoclonal anti - sp18 antibodies. after obtained using rpas system, vh and vl genes were used to assemble scfv gene fragment with a linker primer
應用重組噬菌體抗體庫技術,從分泌小鼠抗牛精子sp18抗體的雜交瘤細胞系中分離總rna ,克隆抗體重鏈和輕鏈可變區基因,加入連接肽引物( linkerprimer )組裝成單鏈抗體scfv ( singlechainfragmentvariable )基因並用rs引物進行擴增, sfi 、 not酶切,回收后與pcantab5e載體相連,轉化e . colitg1宿主菌,構建單鏈抗體文庫。Except for 1. 0 kb hybridization band, other different hybridization bands were also detected which demonstrated that lea3 gene integrated into strawberry genome in different loci or copies
Okb雜交帶外,還有其他片段人小不等的雜交帶。不同的株系形成不同的雜交譜,表明這些轉化植株來自不同的轉化細胞。Establishment and characterization of two hybridoma cell lines secreting anti - osteosarcoma antibody
抗人成骨肉瘤雜交瘤細胞系的建立及其特性鑒定Asymmetric somatic hybridization between mixed wheat and psathyrostachys juncea
混合小麥親本與新麥草不對稱體細胞雜交體系的建立The male sterile lines and their respective maintainer lines of brassica napus, b. campestris and b. juncea were chosen as the parents in interspecific crosses with b. napus, b. campestris, b. juncea and eruca sativa in order to investigate male sterile cytoplasmic effects on the interspecific cross incompatibility
摘要選擇甘藍型、白菜型、芥菜型三種類型油菜雄性不育系和保持系分別與甘藍型、白菜型、芥萊型油菜和蕓芥進行種間雜交,研究十字花科種間雜交親和性雄性不育細胞質遺傳效應。In our work, different hbv pres gene fragments were amplified by pcr, cloned into pgbkt ? vector in yeast two - hybrid system 3, and then transformed ah 109 yeast cells. the results showed that these pres expression products were nontoxic to yeast cells, but all self - activated the yeast two - hybrid system
本研究將hbv前s區不同片斷進行擴增,克隆入酵母雙雜交系統3中的pgbkt7載體,轉化酵母細胞ah109 ,經檢測,證實了hbv前s區不同片斷對酵母細胞無毒性作用,但是都存在自激活作用。Ceu line 2 ) molecular biology reagents of gene cloning 3 ) reagents for northern blot and rt - pcr 4 ) cell transfection kit 5 ) reagents for kinase assay 2
肝癌bel7402細胞系和cos7細胞系2基因克隆相關分子生物學試劑3 northern雜交及rt - pcr相關試劑4分享友人