幾丁質合酶 的英文怎麼說
中文拼音 [jīdīngzhígě]
幾丁質合酶
英文
chitin synthase-
For the high selectivity and safety of chitin synthase inhibitors, they are used for insecticides, fungicides and acaricides
幾丁質合成酶抑制劑由於具有安全、高效等特點,成為農用殺蟲、殺蟎、殺菌劑以及醫藥抗真菌藥物的研發熱點。The engineering bacterium which carried bcih i - chi and i - glu cdna was pcg - ii. two methods of agrobacterium - mediated and gene gun were used to transformate long ya lillium. the results of pcr analysis and southern dot blotting hybridization demonstrated that the chi a nd glu cdna have been intergrated into host genome. at the same time ; compared agrabactenum - mediated method with gene gun method, the transformation frequency of the former was 16. 7 %, while the latter was 50 %, so gene gun transformation method was suitable for long ya liiliwn
用攜帶有幾丁質酶基因和- 1 、 3葡聚糖酶基因的工程菌,通過農桿菌介導法和基因槍轉化法轉化龍牙百合,經pcr和點雜交檢測證明外源基因已經整合到植物染色體中。同時對農桿菌介導法和基因槍法進行比較,發現農桿菌介導法的轉化率為16 . 7 ,基因槍法的轉化率為50 ,因此可能基因槍轉化法更適于龍牙百合的遺傳轉化。Chitin synthase is the important enzyme that converts udp - glcnac to chitin
摘要幾丁質合成酶是生物合成幾丁質的關鍵物質。Producing plant phytoalexins has been recognized as the primary and dominant routines in defending system in cotton, include terpenoid, proteases and hormones in which many enzymes, such as chitinase, has been proved useful against verti - cillium in several plants, but not their functional mechanism
在棉花抗病反應中以植物抗毒素的合成為主要途徑,包括萜類、酶類和激素類3類化學物質,其中酶類物質如幾丁質酶已經在很多植物中被證明具有抗病作用,但是其具體作用途徑仍不清楚。Chi 1 degraded colloidal chitin both endolytically and exolytically with jv - acetylglucosamine, chitobiose and chitotriose as its products while a c - teminal 302 - amino - acid d eleted mutant had its degrading pattern shift to chitobiose, chitotriose and chitotetraose. further research showed a shifting of the minimal length of chitooligosaccharide needed for these two correlated proteins. in the case of full - length chitinase, it was chitotriose while with its truncated form, it turned out to be chitotetraose
進而對chi1和chi1 ~ -做了體外表達、純化及功能的比較分析,首次揭示了幾丁質酶潛在的幾丁結合區對酶作用底物與產物的特異性影響,即chi1 ~ -仍具有較強活性,但其膠體幾丁質降解產物為幾丁二、三、四糖。It encodes a protein of 865 amino acids with a signal peptide at the n - terminus, of which a polycystic - kidney - disease ( pkd ) - like domain, a triosephosphate - isomerase ( tim ) catalytic region, a receptor - for - egg jelly ( rej ) - like domain and two tandem chitin - binding - domains ( chbds ) locate from the n - to c - terminus
根據已知幾丁質酶基因的dna保守序列,利用人工合成的pcr引物和cb101總dna ,擴增出一個約2 . 6kb的片段並克隆到pbluescript ks載體上。分享友人