幾丁質酶 的英文怎麼說
中文拼音 [jīdīngzhí]
幾丁質酶
英文
chitinase-
Research progress on bacillus thuringiensis chitinase
蘇雲金芽孢桿菌幾丁質酶的研究進展Chitinase forming strain is a kind of special microorganisms. this strain can utilize chitin as carbon source to survive and repoduce. and it has the common biochemical ch aracteristics of secreting chitinase. chitinase can degrade chitin into chitin oligosaccharide, chitin disaccharide, and chitin monosaccharide. the application of chitinase and chitin oligo saccharide on plant resistance are extensively reported. moreover researches verified that c hitin oligosaccharide can promot the growth of plant. so chitinase froming strain is a kin d of promising fungi - resistant microorgnanism. therefore, it ' s a very meaningful work to d o more extensive and deeper researches in this respect
而幾丁質酶和幾丁寡糖在植物抗病上的應用已經被廣泛的報道，而且有研究證實幾丁寡糖還能促進植物的生長發育。幾丁質酶產生菌是一類很有前途的抗真菌的微生物，因此，在這方面作更廣泛更深入的研究是很有意義的工作。By transformation with the genes. plant disease biocontrol bacteria bacillus subtil is aplls and b. megaterium ap25 were isolated from wheat field soils collected from south australia and tai an. enzyme activity analysis on chitin agar and abp media showed that b. subtilis aplls secreted chitinase and b. megaterium ap25 secreted endoglucanase, respectively
測序后在genebank上進行序列比較，該基因片段同編號為2634966的枯草芽孢桿菌全序列的2599451到2812870 （功能未知）有85的同源性，但同已發表的13種幾丁質酶的基因（包括枯草芽孢桿菌幾丁質酶基因）的同源性很低，只有30 。The engineering bacterium which carried bcih i - chi and i - glu cdna was pcg - ii. two methods of agrobacterium - mediated and gene gun were used to transformate long ya lillium. the results of pcr analysis and southern dot blotting hybridization demonstrated that the chi a nd glu cdna have been intergrated into host genome. at the same time ; compared agrabactenum - mediated method with gene gun method, the transformation frequency of the former was 16. 7 %, while the latter was 50 %, so gene gun transformation method was suitable for long ya liiliwn
用攜帶有幾丁質酶基因和- 1 、 3葡聚糖酶基因的工程菌，通過農桿菌介導法和基因槍轉化法轉化龍牙百合，經pcr和點雜交檢測證明外源基因已經整合到植物染色體中。同時對農桿菌介導法和基因槍法進行比較，發現農桿菌介導法的轉化率為16 . 7 ，基因槍法的轉化率為50 ，因此可能基因槍轉化法更適于龍牙百合的遺傳轉化。Purification and characterization of a chitinase from endophytic chaetomium spirale nd
35幾丁質酶的純化和性質14 out of 25 strains of biocontrol agent streptomyces showed chitinolytic activity by biological assay. these 14 strains of biocontrol agent streptomyces were detected for chitinase gene using pcr primers derived from the conservative sequence of 17 cloned chitinase genes from streptomyces
此方法相對于幾丁質酶的傳統生物學檢測，具有更好的準確性和靈敏性；而且與生物學檢測相比，使用分子檢測省時省力。So we can say that the red gene may also be used as a reporter gene
該重組菌株既能產生幾丁質酶，又能使菌體在紫外線下呈現紅色熒光。Cloning of chitinase gene from streptomyces roseoflavus and its splicing expression in escherichia coli
玫瑰黃鏈黴菌幾丁質酶基因的克隆及拼接表達Producing plant phytoalexins has been recognized as the primary and dominant routines in defending system in cotton, include terpenoid, proteases and hormones in which many enzymes, such as chitinase, has been proved useful against verti - cillium in several plants, but not their functional mechanism
在棉花抗病反應中以植物抗毒素的合成為主要途徑，包括萜類、酶類和激素類3類化學物質，其中酶類物質如幾丁質酶已經在很多植物中被證明具有抗病作用，但是其具體作用途徑仍不清楚。Aeromonas caviae can secrete several chintinases with different molecular weights. one chitinase gene chil has been cloned and sequenced
豚鼠氣單胞菌（ aeromonascaviae ） cb101能產生多種分子量不同的幾丁質酶。Chi 1 degraded colloidal chitin both endolytically and exolytically with jv - acetylglucosamine, chitobiose and chitotriose as its products while a c - teminal 302 - amino - acid d eleted mutant had its degrading pattern shift to chitobiose, chitotriose and chitotetraose. further research showed a shifting of the minimal length of chitooligosaccharide needed for these two correlated proteins. in the case of full - length chitinase, it was chitotriose while with its truncated form, it turned out to be chitotetraose
進而對chi1和chi1 ~ -做了體外表達、純化及功能的比較分析，首次揭示了幾丁質酶潛在的幾丁結合區對酶作用底物與產物的特異性影響，即chi1 ~ -仍具有較強活性，但其膠體幾丁質降解產物為幾丁二、三、四糖。Four colonies of transformed e. coli dh5 a with clear hydroiyzing zone on the chitin agar were obtained. the gene fragment in these isolates was identified by the methods of plasmid processing. dna sequencing analysis showed that sequence homology between pcr fragment and chromosomal dna of b. subtilis from 2599451 to 2812870 was 85 %, and was 30 % between the fragment and the genes encoding for chitinase of bacillus ( including b. subtilis ) in genebank
測序並序列比較結果表明該基因片段同已發表的枯草芽孢桿菌幾丁質酶和內切葡聚糖酶編碼幕因的克隆及重組芽抱桿菌的構建glyb一apre之間的同源性是最高的，為35 % ;同bacz 』了了ussp . bp23ce1b 、 b . p朋刀us內切葡聚糖酶和b . pol理vxap一1 ， 4一內切葡聚糖酶的編碼基因的同源性只有27 % 。Pcr methodology was adopted for cloning of chitinase encoding genes. based on the sequences of chitinase gene from genebank, the primers for chitinase gene amplification were designed. pcr fragment was ligated with pmd18 ~ t vector and transformed into e. coli dh5 a
盡管同源性比較低，但酶活性檢測發現該基因片段具有幾丁質酶活性，認為此pcr片段含有幾丁質酶編碼基因的全序列或部分序列，此基因片段是一個新基因或基因片段。It encodes a protein of 865 amino acids with a signal peptide at the n - terminus, of which a polycystic - kidney - disease ( pkd ) - like domain, a triosephosphate - isomerase ( tim ) catalytic region, a receptor - for - egg jelly ( rej ) - like domain and two tandem chitin - binding - domains ( chbds ) locate from the n - to c - terminus
根據已知幾丁質酶基因的dna保守序列，利用人工合成的pcr引物和cb101總dna ，擴增出一個約2 . 6kb的片段並克隆到pbluescript ks載體上。Gene cloning and sequence analysis of chitinase gene in wheat seed
小麥種子中幾丁質酶基因的擴增及序列分析Screening and purification of neutral protease with chitinase inhibitor activity
具幾丁質酶抑制活性的中性蛋白酶的篩選與純化Genoraic dna of b. megaterium was partially digested by restriction enzyme sau3a and was used to establish the genomic library in plasmid pbluscripts. selection for endoglucanase positive clones from transformed e. coli was carried out on the cmc medium. seventy - four clones that showed hydrolysis ability on the cmc plate were obtained
用ecori和sall對含有幾丁質酶基因的重組質粒tvchi （含幾丁質酶編碼基因的pmd18一t載體）和穿梭質粒phy300plk進行雙酶切並連接，轉化e . colidhs 。Sds - page and activity staining analysis revealed that all of clones 2, 5 and 9 exhibited a single active band with 83 - kd in size, whereas clone 3 exhibited four different bands with 64 kd, 70kd, 76kd and 83 kd after construcing a series of deleted subclones from the plasmid prepared from clone - 5, a complete nucleotide sequences comprising of 3, 205 bps wes determined. one open reading frame ( orf ) was found in this sequence, which was comprised of 2, 661 bps and could encode a polypeptide with 887 amino acid residues
利用基因分析軟體genetyx對clone - 5質粒的核苷酸序列進行分析，並搜索日本dna數據庫（ dnadatabankofjapan ，簡稱ddbj ） ，推斷clone - 5質粒的幾丁質酶基因含有一個2 ， 261bp的開放閱讀框（ orf ） ，編碼一條887個氨基酸的幾丁質酶蛋白； orf上游有一個啟動子。Microorganism chitinases : molecular biology and potential use as biopesticides
微生物幾丁質酶的分子生物學研究及其應用Studies on transgenic oilseed rape brassica napus plants transformed with - 1, 3 - glucanase and chitinase genes and its resistance to sclerotinia sclerotiorium
葡聚糖酶及幾丁質酶基因的轉基因可育油菜及其抗菌核病的研究分享友人