核酸雜交 的英文怎麼說

中文拼音 [hésuānzájiāo]
核酸雜交 英文
hybridization of nucleic acid
  • 核 : 核構詞成分。
  • 酸 : 酸構詞成分。
  • 雜 : Ⅰ形容詞（多種多樣的; 混雜的） miscellaneous; varied; sundry; mixed Ⅱ動詞（混合在一起; 攙雜） mix; blend; mingle
  • 交 : Ⅰ動詞1 （把事物轉移給有關方面） hand over; give up; deliver 2 （到某一時辰或季節） reach （a cert...
  • 核酸 : [生物化學] nuclein; nucleic acid核酸聚合酶 nucleic acid polymerase; 核酸酶 nuclease; 核酸內切酶 [生物化學] endonuclease
  • 雜交 : [生物學] hybridize; cross; hybridization; cross breeding
  1. More and more fluorescent signal can be collected with the pcr reaction carry on. the method is more automatized and much less time consumption ( only 3 hours from nucleotide hybridization capture to result found )

    這樣利用熒光信號積累可以實時監測整個pcr進程，實現了pcr擴增和核酸雜交以及熒光電信號放大檢測同步進行的自動化檢測技術；實時熒光pcr技術具有更大的優越性： l ）不需要pcr后處理，
  2. Interspecific hybridization of nucleic acids is a method of this type currently being developed.

    核酸的種間雜交便是當前正在發展的這樣一種方法。
  3. Dig labeled probe hybridization with solid pcr product was performed as well as electrophoresis of liquid product, this method combines rna purification, pcr amplification and nucleotide probe hybridization detection together and has many advantages including better rna purity, less time consumption, reliable positive reaction and 10 times sensitivity as rt - pcr gel running detection, reduce false positive result, unpurified nucleotide requirement, loug infected plant organism can be detected by solid hybridization

    2 ）結果可靠，雜交特異性誘捕目的片段，同時去除了核酸中存在的pcr抑制物質，減少了因核酸提取不純造成的漏檢現象，結果輸出通過雙重判定保證了結果的可靠性。 3 ）靈敏度高，通過雜交進行結果判定，靈敏度比傳統的rt干cr大約高10倍。
  4. Lastly by using the technique of dot blot hybridization, the genome dna of chlamydia was detected with the probe of momp gene labeled with dig - 11 - dutp by using the way of random primer. the results showed the degree of sensitivity of the probe was 10 pg and other pathogens could not be detected by this probe. by comparing the diagnostic ways of nucleotide probe and fc, the technique of nucleotide probe were proved to have high sensitivity and speci fi city

    最後，用地高辛隨機引物法標記成momp基因核酸探針，斑點雜交檢測衣原體基因組dna ，靈敏度可達10pg ，且不能檢出其它病原體的核酸。將核酸探針法與補體結合反應法對衣原體感染的診斷進行比較，初步證明該探針具有較高的敏感性與較強的特異性。
  5. One was cloning, sequence analysis and expression of the fragment containing the b and c antigenic sites locating at the 5 " terminus in spike gene of tgev in prokaryotic expression system ( fused with gst ), the other was preparation of non - radioactive probe labeled by digoxigenin for detecting the rna extracted from tgev by assay of dot - blot

    為了鑒別診斷tgev與prcv及對tgev進行流行病學調查，本研究採用原核表達系統（ gst融合表達系統）表達tgev纖突蛋白（ s蛋白）中含有b和c抗原位點的多肽，並且制備了非放射性地高辛標記的核酸探針，通過斑點雜交（ dot - blot ）檢測tgev核酸rna 。
  6. The divergent sequences of rdna from s. costatum are used to design primers meeting the requirements of the rfq - pcr. seven pairs of primers are designed and designated as primer 1 ( f / r ) ~ primer 7 ( f / r ), respectively, among which primer 1 ( f / r ), primer 2 ( f / r ), primer 5 ( f / r ), primer 6 ( f / r ) showed high level of specificities to s. costatum. then, the pcr products primed by primer6 ( f / r ) are sequenced

    首先以中肋骨條藻的rdna序列為設計種特異性引物的靶區域，共設計出7對適合rfq - pcr的引物（依次命名為primer1 （ f r ） primer7 （ f r ） ） ，並用常規pcr實驗初步證明其中有4對引物（ primer1 （ f r ） 、 primer2 （ f r ） 、 primer5 （ f r ） 、 primer6 （ f r ） ）可作為中肋骨條藻特異性引物的候選者；然後測定了以primer6 （ f r ）為引物的pcr產物的序列，序列分析表明，中肋骨條藻的pcr產物序列與其他藻的pcr產物序列差別較大，從中可設計出滿足rfq - pcr需要的taqman探針（命名為taqman6 ） ；進一步的核酸雜交實驗表明， taqman6隻與中肋骨條藻的pcr產物雜交，不與其他藻的pcr產物雜交。
  7. 3. using scanning tunnel microscopy ( stm ) to observe microcosmic change between biomolecule and gold particle on the surface of lsaw biosensor during the process of probe immobilization and hybridization, also the naked gold membrane

    3 .利用掃描隧道顯微鏡觀察傳感器裸金膜表面、探針固定、核酸雜交過程中生物分子與金顆粒之間的微觀變化。
  8. System for detecting single nucleotide polymorphisms based on hybridization probes

    熒光雜交探針技術檢測單核苷酸多態性
  9. To device a primer pairs and amplify the full length pstvd by rt - pcr, the positive rna extraction from tuber sample was used as template. the product of rt - pcr was purified and connected to the plasmid pmd 18 - t vector

    Cdna核酸斑點雜交反應（ nash ）檢測pstvd方法準確、靈敏度高，一次檢測樣品數量多，且對于異地樣品檢測非常方便，是以往其它檢測方法的有效補充。
  10. 5. the mpcr - rflp assay was useful for its reliability in monitoring hbv ymdd mutants. melting curve assay and pcr microplate hybridization - elisa assay should be further improved to increase their sensitivity and specialty

    5 . mpcr一rflp法檢測ymdd突變株具有較好的可靠性和可行性，是監測拉米夫定耐藥株的一種非常有效的方法;熔解曲線法和pcr微板核酸雜交法需要進一步完善以提高敏感性和特異性。
  11. The effects of glp - 1 ( 7 - 36 ) nh2 on insulin secretion glp - 1 ( 7 - 36 ) nh2 with the concentration of 2. 5nmol / l, 5. 0nmol / l, l0. 0nmol / l, 20. 0nmol / l, 40. 0nmol / l respectively were added to the medium as different experimental groups, 24 hours later, insulin amount are 68. 76 ? 1. 71 72. 30 ? 3. 13 104. 16 ? 5. 57 110. 98 ?. 29 111. 58 ? 0. 65miu / l respectively, and the insulin account is 55. 53 ?. 63miu / lin the control group. there was no significant difference between the groups with 2. 5nmol / land 5. 0nmol / l glp - 1 ( 7 - 36 ) nh2 respectively ; and there was not significant difference among the groups with lo. onmol / l, 20. 0nmol / l and 40. 0nmol / l glp - 1 ( 7 - 36 ) nh2 respectively. but the difference is significant between experimental groups and control group ( p < 0. 05 ). the data show that with the rising concentration of glp - 1 ( 7 - 36 ) nh2, there is an increasing amount of insulin

    對照組培養液中不含g廿一1 （ 7一36 ） nhz ，實驗組培養液中含有20nmol / lglp一1 （ 7一36 ） nhz ，培養24h后，用0 . 25 %胰蛋白酶消化胰島分散細胞，塗片后利用針對胰島素mrna的寡核甘酸探針進行細胞原位雜交， dab顯色，高清晰度病理圖文分析系統（ highpathologiealimageanalysissystem ， hp認s ）對細胞著色的平均光密度（ mean即tiealdensity ， mod ）量化分析，觀察實驗組和對照組胰島素mrna的表達情況。
  12. Hbv genotype was determined by the restriction fragment length polymorphism analysis in patients with chronic hbv infection in 5 cities of fujian province. 2. the sensitivities and specialties of melting curve assay and pcr microplate hybridization - elisa assay were compared with mpcr - rflp and sequence analysis for the detection of hbv ymdd mutants in 44 serums from patients receiving lamivudine monitherapy with viral breakthrough

    應用熔解曲線法和pcr微板核酸雜交- elisa法對44例接受拉米夫定治療過程中出現病毒學反跳時的血清進行ymdd突變株的檢測，並與測序法和mpcr - rflp法比較它們的敏感性、一致性。
  13. The author reviewed the detection measures of prunus necrotic ringspot virus, and related the research progression of the pathogen detection technology inside and outside. the template amplication technology include pcr assays ^ nasba and so on. the cdna and crna probe which labeled with the isotope % biotin or dig, the offset probe and the peptide probe can be applied to magnify the signal. pcr - gene scan assays and pcr agilent chip chamber combine the template amplication and the signal magnification

    本文回顧了李壞死環斑病毒的檢測方法，較全面地評述了國內外病原物檢測技術研究進展：在模板的擴增有各種pcr技術、 nasba技術；在信號的放大有同位素、生物素或dig標記的cdna和crna探針，分支探針和肽核酸探針；模板擴增和信號放大相結合的有pcr -基因掃描技術、 pcr安捷倫晶元實驗室技術；模板擴增和雜交以及信號放大相結合的有pcr - elisa技術、實時熒光pcr技術、生物晶元技術。
  14. Part one the studies of piezoelectric biosensors and detection of staphylococcal enterotoxins chapter one the progresses in piezoelectric biosensors and detection of staphylococcal enterotoxins section one the progresses in piezoelectric biosensors in this section, a review is reported with 69 references about the piezoelectric biosensors, including the principle and constitutes of pebs, the classification of pebs, surface modification technique in pebs and the application of pebs in immunoassay, detection of the gene and microorganism, coupling with other techniques, moreover, the development of pebs was involved

    引用參考文獻69篇。第二節金黃色葡萄球菌及其毒素檢測研究進展該部分內容主要介紹了在葡萄球菌分類與分型、金黃色葡萄球菌毒素學研究以及金黃色葡萄球菌及其毒素檢測等方面的研究進展，重點對金黃色葡萄球菌及其毒素檢測方法：生物學檢測方法、免疫血清學方法、聚合酶鏈反應技術、核酸雜交技術、生物傳感器技術和超抗原技術在近幾年的研究進展做了評述，引用文獻69篇。火nxia即。
  15. Gene chips is high density probe array which is composed of nucleic acid or nucleic acid band arranged on the solid support media according to the definite order. it can detect unknown molecule by nucleic acid hybridize principle

    基因晶元是將核酸或核酸片段按照一定的順序排列在固相支持物上構成高密度探針陣列，利用核酸雜交原理檢測未知分子。
  16. Therefore, the determination of seb is very important for food hygienic analysis as well as for clinical analysis. nucleic acid hybridization technique is one of the widely - used methods in molecular biology and gene technology. the present work has developed piezoelectric biosensors used in the detection of seb dna by tacking the piezoelectric quarts crystal as a sensitive component while synthetic oligonucleotide probe as recognize molecule

    其中b型葡萄球菌腸毒素（ seb ）是一種通常條件下更穩定，毒性最強的毒素，而核酸雜交技術則是分子生物學和基因工程中最常用和最基本的方法之一，因此本論文以該毒素的產毒基因為檢測對象，以壓電石英晶體為敏感元件，以合成的寡核苷酸探針為識別分子，構建了用於seb基因檢測的壓電生物傳感器。
  17. Then hydrate and roast " to fix the probes, ready for use 6. block and hybridizate block and wash the prepared slides by the method developed in our lab

    6 .封閉與核酸分子雜交按本室方法，將制備好的寡核昔酸微陣列，清洗，封閉。
  18. The accuracy and stability of the typing result typing results match the set results, and in 5 hybridization, the repeated rate is 91. 2 %

    3寡核營酸晶元分型結果的準確性與穩定性分型結果與dna測序相符合，重復5次雜交重現率91 . 2 % 。
  19. To improve the performance of assays on the oligonucleotide microarray, the factors that influence the hybridization effects such as surface chemistry, probe size, spacer length, hybridization conditions etc were intensely studied and optimized

    為改善寡核苷酸晶元的分析性能，對影響晶元雜交結果的因素，如片基表面的化學處理、探針的長度、間隔臂的長度、雜交條件等，進行了深入的研究和優化。
  20. The cdna expression library that contains no intron theoretically include all expressed genes and conserve resource genes permanently. lt can be used to find out and clone some genes which can express particular protein with modern molecular biological techniques, such as immunological screening, drug - prob screening, southern et al. lt is very important to study the life nature of plasmodium falciparum in molecular level. with the developments of these studies, the drug - resistant mechanism of the plasmodium falciparum and the genes of some specific medicine binding protein can be made well - known. at the same time, the researches will do good to explain the mechanism of some specific medicines in order to design and screen new anti - malaria drugs

    建立cdna表達文庫在一次永久保存基因資源的同時，可以利用功能篩選、免疫學篩選、藥物探針篩選、 southern雜交和大規模序列測定等現代分子生物學技術尋找特異性活性蛋白基因，進而克隆和表達這些基因，對從核酸及蛋白質等分子水平研究瘧原蟲的生命活動規律，對揭示其抗藥性分子機理，搞清某些特效藥物結合蛋白的基因及此類藥物的作用機制，對新型抗瘧藥物的合理設計及篩選都具有極其重要的現實意義。
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