片段疫苗 的英文怎麼說

中文拼音 [piānduànyìmiáo]
片段疫苗 英文
split vaccines
  • 片 : 片構詞成分。
  • 段 : Ⅰ量詞（部分） section; segment; part; paragraph; passage Ⅱ名詞（姓氏） a surname
  • 疫 : 名詞（瘟疫） epidemic disease; pestilence
  • 苗 : 名詞1 （初生的種子植物） seedling; sprout; shoots 2 （初生的飼養動物） the young of some animals ...
  • 片段 : part; passage; fragment; extract; segment; bit; episode; snatch; section
  • 疫苗 : vaccinum; vaccine; vaccin
  1. By using aa 385 - 365 fragment, an elisa system for the evaluation of post - e2 - vaccination humoral immune responses was also established, and was successfully applied to recombinant vaccinia virus - and dna - based vaccine research

    利用aa385 - 565片段，建立了e2疫苗免疫后抗體反應的elisa評價體系，並已成功用於重組痘苗病毒疫苗和dna疫苗的研究。
  2. Vp3 gene of hl isolate of goose parvovirus derived from recombinant piasmid pproex htb - vp3 was subcloned into ecorl site of psy538, and the reporter gene lacz with promoter pll was cloned into smai site of recombinant piasmid. both vp3 gene and lacz gene were cloned into noti site of psy681, recombinan fpv transfer vector containing vp3 gene of gpv was obtained. the result is basis of construction of recombinant fpv expressing vp3 gene of gpv and gpv genetic engineering vaccine

    本研究另從含有gpvh1分離株主要結構蛋白vp3基因的重組質粒pproexhtb - vp3中切取gpvh1株vp3基因片段，將其亞克隆于psy538的ecori位點，然後將帶有痘苗病毒啟動子p11的lacz報告基因平端克隆于上述重組子的smai位點，再用noti切下同時含有vp3基因和lacz報告基因的片段，再亞克隆于psy681的noti位點，構建出含有vp3基因的重組禽痘病毒轉移載體，為構建表達vp3基因的重組禽痘病毒從而制備gpv基因工程疫苗奠定了基礎。
  3. These findings of the specific ctls epitopes in irbp and pedf may lead to improved understanding of the pathogenesis of uveitis. our study also provides a strategy to identify specific ctls epitopes within tumour antigen, which is helpful to make tumour vaccine for the patients

    本研究也為尋找腫瘤抗原中具有誘導特異性ctls能力的肽片段提供了一種策略，為制備肽片段腫瘤疫苗提供了理論基礎。
  4. Methods : the rearranged gene fragment coding tcr y v region of the jurkat cell line was obtained by rt - pcr technique the pcr product was cloned into the eukaryocytic expressive vector pcdnas to construct pcdna3 / tcr y. after confirmed by sequncing. pcdnas / tcr y plasmids were amplified in bacteria extracted by alkaline lysismethod

    方法：本文採用rt ? ? pcr的方法擴增jurkatt淋巴瘤細胞特異性重排的tcr可變區基因片段，克隆到真表達載體pcdna _ 3中，經序列測定無誤后，堿裂解法大量提取質粒，制備dna疫苗。
  5. As well as in eukaryocyte ( hepg2 and cos - 7 ), then detect their antigenity as a basis study and explore of the choice of immunogen for preventive and therapeutic vaccines of hepatitis b. methods : the gene fragments coding 152aa ( si ) and 124aa ( s2 ) of the carboxyl terminus of hbsag were amplified by pcr from plasmid pecob6 with a pair of primers containing different endonuclease sites and were cloned into multiple cloning sites of plasmid pbks ( + )

    為乙型肝炎的預防和治療性疫苗免疫原的選擇進行初步的研究和探討。方法：本研究利用聚合酶鏈反應（ pcr ） ，通過設計帶有不同酶切位點的一對引物，從質粒pecob6特異性擴增hbsag蛋白羧基末端152個氨基酸（ s1 ）和124個氨基酸（ s2 ）的基因片段，分別將二者克隆到質粒pbks （ + ）的多克隆位點，篩選重組克隆。
  6. This strain ' s virulence was judged by mean death time of chick embryos ( mdt ), intracerebral pathogenicity index in day - old chicks ( icpi ) and intravenous pathogenicity index in 6 - week - old chickens ( ivpi ) and it was found to be the virulent strain. at last, it was tested by the recurrent infection and found that it was the newcastle disease virus ( ndv ), and it was named hbg - 1 strain. in order to find the difference of the cleavage site of this strain with f48e9 and ? 30 strain, a part of the cleavage site of fusion protein gene fragment was amplified by rt - pcr using a primer and sequenced. the sequence analysis showed this strain had low homology with f4ge9 and cso. a phylogenetic tree based on the published sequences of ndv reference strains was constructed and showed the isolated strain hbg - 1 belonged to the genotype w ndv, a novel genotype ndv

    為了進一步探尋分離株與標準株的異同，又採用rt - pcr方法，擴增獲得分離株f _ o裂解位點附近的基因片段，經測序后與國際上已發表的新城疫病毒的核酸序列進行比較，結果表明其與標準株和疫苗株之間的同源性較低，僅為82 86之間。經系統發育進化樹分析后，判定該分離株為新城疫病毒（ ndv ）基因型。運用計算機軟體對其裂解位點處的氨基酸序列進行預測和分析，結果表明該分離株為新城疫病毒強毒株並具有基因型的典型結構特徵。
  7. In this study, a 1. 7kb kpni fragment and a lacz gene expression cassette carrying the e. coli lacz gene under the control of sv40 promoter were inserted into the transfer vector pbdtk - uni ( a 277bp acci - accl fragment in the tk gene was deleted ). the new transfer vector was called puni - lacz. the transfer vector puni - lacz and purified genomic dna of strain bartha - k61 were used to cotransfect vero cells using lipofectin transfection procedure

    本研究以呈ge ~ -表型的經典弱毒疫苗bartha - k61株為親本株，在通用prv轉移載體pbdtk - uni的基礎上，在其多克隆位點中插入由sv40早期啟動子控制下的lacz基因表達盒，同時將下游同源臂增加了一個1 . 7kb的kpni片段，使上下游同源臂的長度都超過了1kb ，構建了一個新的轉移載體puni - lacz 。
  8. For creating a reasonable displaying method, momp gene fragment and pbv221 are recombined oritationally they are transformed into e. coli jm109 ( de3 ). a expressing protein was got with momp antigen. this study establishes the foundation for advance research of momp

    設計一條較為合理的表達方案，將momp基因片段與pbv221定向重組轉化大腸桿菌jm109 （ de _ 3 ） ，得到具有momp抗原性的表達蛋白，為研究momp的生物學功能以及研製沙眼衣原體基因工程亞單位疫苗奠定了基礎。
  9. To explore the feasibility of ib edible vaccine, we have transformed si gene of ibv into potato and researched the immunogenicity of it ' s expression product. the findings are as follows : 1. a pair of primers for ibv si gene were designed and synthesized according to the published nucleotide sequence of ibv si gene and multiclone sites of expression vector pbi121

    為了探索ibv可食性疫苗的可行性，我們進行了轉基因馬鈴薯表達ibv免疫原基因及其表達產物免疫原性的研究，取得以下結果： 1根據周繼勇等報道的ibv - zj971毒株s1基因核苷酸序列和pbi121植物表達載體的多克隆位點，設計併合成引物，以含s1pbs質粒為模板擴增s1基因，將擴增片段定向克隆到pbi121質粒的35s啟動子下游。
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