病毒核質 的英文怎麼說

中文拼音 [bìngdúhézhí]
病毒核質 英文
vironucleon
  • 病 : Ⅰ名詞1 （疾病; 失去健康的狀態） illness; sickness; disease; malum; nosema; malady; morbus; vitium...
  • 毒 : Ⅰ名詞1 （對生物體有害的性質或物質; 毒物） poison; toxin 2 （毒品） drug; narcotics 3 （姓氏） a s...
  • 核 : 核構詞成分。
  • 質 : Ⅰ名詞1 （性質; 本質） nature; character; essence 2 （質量） quality 3 （物質） matter; substance;...
  • 病毒 : [醫學] virus; inframicrobe （濾過性）
  1. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  2. The lead researcher, joseph hibbeln of the us national institutes of health, and the bristol scientists, including professor jean golding, compared the amount of fish eaten by pregnant mothers with the development of their children up to the age of eight

    對孩子的行為和智力的新的研究表明聽從了美國控制結核病的「建議」 ，或害怕汞中毒而不吃魚的婦女，可能缺少大腦發育所需的營養物質，從而損害到他們的孩子。
  3. In this study five recombinant plasmids containing hn ( hemagglutinin - neuraminidase ) genes of ndv ( newcastle disease virus ) were constructed, hn genes were amplified by reverse transcriptase - polymerase chain reaction ( rt - pcr ) and were inserted directly into eukaryotic expression plasmid pcdna3

    應用反轉錄-聚合酶鏈反應（ rt - pcr ）獲得兩株新城疫病毒（ ndv ）血凝素-神經氨酸酶（ hn ）基因cdna ，然後定向插入真核表達質粒pcdna3中，構建了含hn基因的重組質粒。
  4. Results in the 260 slices of liver biopsy, there were 20 ones of acute viral hepatitis, 78 ones of chronic hepatitis, 79 ones of hepatocirrhosis, 28 ones of primary liver cell cancer, 27 ones of liver karyon heterogeneity, 20 ones of liver metastatic adenocarcinoma, 12 ones of fatty liver, five ones of alcohol liver, three ones of liver cyst, two ones of congenital bile duct atresia, six ones of illegible structure, 20 ones without liver cell or with few scattered liver cell

    結果260例肝穿活檢組織病例中，急性（病毒性）肝炎20例；慢性肝炎78例；肝硬化39例；原發性肝細胞癌28例；肝細胞核異質27例；肝轉移性腺癌20例；脂肪肝12例；乙醇肝5例；肝囊腫3例；先天性膽管閉鎖2例；穿刺組織結構模糊6例；未穿到或僅穿到少量肝細胞20例。
  5. The loop sequence of mb1 and mb2 were the anti sense and sense sequence ofing1, respectively the sequence of mb3 was a piece of ssrna sequence in tobacco mosaic virus, which had no analogical to human gene. mbl was the most suitable probe because mbl had the highest fluorescence enhancemen after hybridizing wtth rna extrated froin normal cell

    第三章，根據一種常見的病毒煙草花葉病毒（ tmv ）的核酸序列設計了分子信標熒光探針，由於tmv的遺傳物質是rna ，分子信標又具有很高的特異性和靈敏度，因此感染了病毒粒子的植物葉片在經過簡單處理后，可用分子信標檢測葉片上。
  6. Differential diagnosis included cerebral toxoplasmosis, cytomegalovirus ( cmv ) encephalitis, primary cns lymphoma, progressive multifocal leukoencephalopathy, fungal abcess due to candida, aspergillus, or cryptococcus, varicella - zoster virus encephalitis or vasculitis, herpes simplex encephalitis, tuberculosis ( m. tuberculosis ), and kaposi ' s sarcoma

    其它的診斷包括：腦弓形體病，巨細胞病毒（ cmv ）型腦炎，原發中樞系統淋巴瘤，漸進性多灶性腦白質病，假絲酵母菌，麴菌或隱球菌所致真菌性膿腫，水痘帶狀病毒型腦炎或脈管炎，單純皰疹腦炎，肺結核（多發性結核） ，和卡波西肉瘤。
  7. Such examples are given as the blood filtering principal of hemoglobin, catalysis of enzymes, immune recoglization, prion, glycoprotein and the relationship of structure and function of membrane protein, et al, as well as the applications to medicine

    在簡要介紹結構生物學的研究方法的基礎上，主要從分子水平闡述蛋白質和核酸的結構原理、相互作用、結構與功能的關系，通過具體實例闡述血紅蛋白的輸氧機制、酶的催化機制、免疫分子識別、朊病毒、糖蛋白、生物膜的結構功能關系等，以及結構生物學在醫學上的應用。
  8. Many cucurbitaceae plants were known to he chinese medicine herbs and contain ribosome - inactivating proteins ( rip ), a group of toxic proteins that have been proposed and demonstrated to play potential use as toxins in the therapy of a variety of human tumors and viruses including hiv

    核糖體失活蛋白（ ribosome - inactivatingproteins ， rip ）具有抗腫瘤和抗病毒等醫用價值，以及抗植物病毒和病原真菌等農用價值。它是一種多活性的物質，不同rip具有各自獨特的功能，其潛在活性還在不斷發現之中。
  9. In immunocompetent hosts, jc virus remain dormant in nuclei of rare scattered glial cells in brain and spinal cord parenchyma

    在免疫功能正常的宿主， jc病毒主要存在於腦、脊髓的少數神經膠質細胞的核內。
  10. In order to investigate the genomic organization of the single - nucleocapid nucleopolyhedrovirus of helicoverpa armigera, the ecori - n fragment located at 54. 8 - 59. 3 kbp of the viral genome was sequenced. the fragment contained 3762 bp helicase gene potentially encoding a protein with a molecular mass of 146 kda

    對棉鈴蟲單核衣殼核多角體病毒（ helicoverpaarmigerdsingle - nucleocapsidnucleopolyhedrovirus ， hasnpv ）基因組中ecori ? n片段進行序列分析，獲得了完整的解螺旋酶基因（ hel ） ，其開放閱讀框大小為3762bp ，編碼一個分子量為146kda的蛋白質。
  11. The protein product of meq gene was highly expressed in the nuclei of recombinant baculovirus infected sf9 cells when using an anti - meq monoclonal antibody ( mcab ) 23b46 to run the immunofluorescence assay ( fa ) ; the expression quantity and if staining patterns differed with different times post - infection ( pi ). the results of western blotting and immunoprecipitation test showed there were two specific bands around 60 kd. the results of the study demonstrated that the baculovirus / insect cell system is effective to be used to express nuclear protein of virus

    結果發現:本表達系統產生的meq蛋白可被重組痘病毒表達的meq制備的單抗23b46所識別;在感染細胞中， meq蛋白僅局限於細胞核內，而且隨著感染后（ pi ）時間的增加，具有從核質向核仁和核膜轉移的趨向; w已stemblot和免疫沉澱試驗均證實重組桿狀病毒感染細胞裂解物中出現有兩條大小約為60kd的特異帶。
  12. Salmonella typhimuriwn, one of the invasive bacterial species, can be attenuated without loss of invasiveness and thus used for delivery of eukaryotic expression vectors into host cells in vivo. the recombinant plasmid containing the target gene is released inside the host cells and gain entry into the nucleus, resulting in expression of encoded antigens and subsequent induction of humoral and cellular immune responses

    沙門氏菌（ salmonellatyphimurium ）是一種較為常見的侵襲性胞內菌，通過基因工程方法減毒后對宿主致病性顯著降低，但仍保留良好的侵襲力，可直接將真核表達質粒攜帶進入動物細胞內表達相應的蛋白而誘導特異性的免疫應答反應。
  13. All the result showed that ndv f48e9 strain has its own speciality compared with other five ndv strains, and there were many difference between velogenic strains and lentogenic strains. so the infectious cdna of rnesogenic strains and lentogenic strain was far from enough to understand the replication, pathogenicity of ndv and the interaction between ndv and host cells, and the infectious cdna of velogenic strains ( eg. f48e9 ) was required to explain the relationships between structure and function

    本研究成功地獲得了ndvf48e9 t因組的核昔酸序列，並構建了表達ndvf48e9基因組cdna的低拷貝表達載體休f48e9 ，為構建新城疫病毒強毒株f48e9株的感染性cdna奠定了物質基礎，進一步研究ndv的生物學特性、結構與功能的關系；進一步探討影響ndv毒力的因素、以及研製新型疫苗載體提供了可靠保證。
  14. The expression conditions of e2 gene in p. pastoris were optimized, the results indicated that the peak obtained after 72 hours ; pattern of inhibition / induction could improve expression level ; the best ph value were between 7. 5 and 8. 0 and the optimized methanol - induced concentration was 2 % - 3 % the e2 genes of the prevalent strain ( guangxi yulin strain ) and c strain derived from rabbit spleen tissue were amplified and cloned into e. coli the expression vector pproex - htb respectively, the recombinant plasmids pproex - gxyl and pproex - c were obtained and then were transformed into the dh5a e. coli competent bacteria respectively, the recombinant bacteria could express the major antigen region of e2 gene, the expression yields amount to 35 % and 38 % repectively

    豬瘟病毒ez基因的原核表達： pcr擴增出當前豬瘟流行野毒株，中國豬瘟兔化弱毒（ c株）兔脾組織毒ez基因的主要抗原區，將其克隆到原核大腸桿菌表達載體pproex htb中誘導表達，經sds page檢測表明，重組質粒能表達ez基因主要區蛋白， westernblot檢測表明，誘導表達蛋白與豬瘟陽性血清發生特異性反應，表達量為35和38 ，可用於基因工程診斷抗原。
  15. In this research, the gpv hl isolate was propagated with 13 day ' s duck embryos. a pair of primers gflgr used to arnplify vp3 gene was designed using oligo4. i software according to the whole nucleotide sequence of gpv b isolate published by zadori. the major structural protein vp3 gene was amplified from the dna of gpv hl isoiate by polymerase chain reaction ( pcr ), and then cloned into pmdl8 - t vecter

    根據zadori等發表的gpvb株全基因核苷酸序列，藉助oligo4 . 1軟體設計了1對用以擴增主要結構蛋白vp3基因的引物gf / gr ，通過pcr技術，從病毒基因組dna中擴增出病毒主要結構蛋白vp3完整基因片段，經酶切鑒定后直接與pmd18 - t質粒載體連接。
  16. These anthraquinones are extremely cytotoxic ( they fight against ) towards a broad spectrum of bacteria, fungi, and viruses that include pneumonia, e coli, blood infections, diarrhea, skin infections and ( tuberculosis

    蒽醌是一種極為有效的廣譜類抗細胞毒素物質，可抗擊多種細菌、真菌、病毒，包括導致肺炎的細菌和病毒、大腸埃希氏桿菌，以及導致血液感染、腹瀉、皮膚感染和肺結核的微生物。
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