白芥子酸 的英文怎麼說
中文拼音 [báijièzisuān]
白芥子酸
英文
sinapinicaicd-
Ip3 - ip3 receptor ( ip3r ) interaction mediates the release of ca2 + from the endoplasmic reticulum in response to many different extracellular stimulus. for higher plants, however, though it is now generally accepted that ip3 participates in signal transduction in many important cellular processes, only limited evidence is available for the presence and properties of the ip3r - like protein so far. here, using the immunological methods with an antibody raised against a mammalian inositol 1, 4, 5 - triphophate receptor ( type 1 ), we found that, 1 ) the antibody across - reacted the proteins with about 200kd in microsomes from oryza sativa and about 200kd from arabidopsis thaliana respectively
本實驗用sds - page電泳和免疫印跡的方法，用哺乳動物大鼠三磷酸肌醇受體的多肽做抗體對類三磷酸肌醇受體蛋白鑒定，結果表明：抗體與水稻和擬南芥微粒體蛋白分子量大約為200kd的蛋白交叉反應，同時還發現在水稻微粒體蛋白62kd和擬南芥微粒體蛋白45kd處有交叉反應的蛋白條帶存在，表明在植物中有類三磷酸肌醇受體蛋白的存在；用免疫膠體金方法，發現類三磷酸肌醇受體蛋白主要分佈於液泡膜和細胞質膜上。Huangyal4 was complete nucleotide sequence of 1 854 bp with a nucleotide orf ( 1575 bp ), which encoded a protein consisting of 524 aa with molecular weight of 62. 2 kda and pi of 8. 96. strongly basic ( + ) amino acids, strongly acidic ( - ) amino acids, hydrophobic amino acids and polar amino acids of the protein were 13. 74 %, 11. 64 %, 36. 45 % and 22. 70 % respectively, and predicted secondary structure of the protein revealed many conserved domains such as n - glycosylation site, protein kinase c phosphorylation site, casein kinase ii phosphorylation site, n - myristoylation site, camp - and cgmp - dependent protein kinase phosphorylation site, tyrosine kinase phosphorylation site and a cytochrome p450 cysteine heme - iron ligand signature which was typical of cytochrome p450. a - helix and b - sheet of the protein is 47. 7 %, 45. 0 % respectively
Huangya14 ）為材料分離克隆到一個細胞色素p450基因，命名為bccyp86mf5 ， cdna全長1854bp ，含1575bp的完整開放閱讀框，編碼524個氨基酸，其編碼蛋白質的分子量為61 . 2kda 、等電點為8 . 96 ；堿性氨基酸、酸性氨基酸、疏水氨基酸和極性氨基酸分別占總氨基酸的13 . 74 、 11 . 64 、 36 . 45和22 . 70 ；二級結構預測包括n -糖基化位點、依賴于camp和cgmp的蛋白激酶磷酸化位點、蛋白激酶c磷酸化位點、酪蛋白激酶磷酸化位點、酪氨基酸激酶磷酸化位點、 n -豆蔻酰化位點和細胞色素p450的典型區域，半胱氨酸亞鐵血紅素配體信號區等， -螺旋和-折疊分別佔47 . 7 、 45 . 0 ；與bccyp86mf1基因的氨基酸序列同源性達到95 . 2 ，與擬南芥cyp86c4的達到85 . 9 。
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