移蠶 的英文怎麼說
中文拼音 [yícán]
移蠶
英文
transferring silkworms-
The pbacph - egf plasmid dna was used to co - transfect bmn cell with the modified bombyx mori baculovirus bm - bacpak dna, which was first linearized by aocl. after two rounds of plaque isolation, the recombinant virus ( named bmbacph - egf ) was further verified with pcr and dot hybridization. the recombinant bmbacph - egf virus was used to infect bmn culture cells at a moi of 10
重組轉移載體dna與經bsu36i酶切線性化的修飾型病毒bm - bacpakdna共轉染家蠶bmn細胞，兩輪空斑篩選和純化，獲得重組病毒rbmbacph - egf ，經pcr擴增、 dna點雜交等方法鑒定證實重組病毒中已正確插入ph - egf融合基因。Gottsch jd. liu sh, minkovitz jb. et al. autoimmunity to a cornea - associated stromal antigen in patients with mooren ' s ulcer. invest ophthalmol vis sci, 1995, 36 : 1541
王錚.陳家祺，陳龍山，等.板層角膜移植聯合環孢黴素a滴眼治療蠶蝕性角膜潰瘍.中國實用眼科雜志， 1996 ， 14 : 283Then bm cell line was co - transfected with parental bm - npv dna and the transfer plasmid pvl - nk, the recombinant virus was then obtained subsequently with routine procedure. nattokinase was expressed in silkworm larva by injection with the recombinant virus
將nk基因克隆至轉移載體pvl - 1393中後用常規方法獲得重組病毒，感染家蠶后納豆激酶獲得正確表達。Fusion gene by pcr was inserted into bombyx mori baculovirus transfer vector pbacpak. 8 and contransfected with lineared dna of bm - bacpak6 virus into bmn cells. the homologous recombination occurred inside the cells, and the recombinant virus bacpak - 6aa - hgm - csf was expressed, as identified by pcr and southern hybridization. the bmn cells and the fifth instars were infected by the recombinant virus bacpak - 6aa - hgm - csf
本研究首先通過pcr將家蠶桿狀病毒多角體蛋白起始密碼子后的18個堿基引入到hgm - csf基因的5 』端之前，然後將融合基因重組與家蠶桿狀病毒轉移載體pbacpak8中，獲得重組轉移載體pbacpak8 - 6aa - hgm - csf ，並與線性化bm - bacpak6dna共轉染家蠶細胞株，獲得重組病毒bacpak - 6aa - hgm - csf 。分享友人