簡並寡核苷酸 的英文怎麼說
中文拼音 [jiǎnbàngguǎhésuān]
簡並寡核苷酸
英文
degenerate oligonucleotide-
All kind of oligonucleotides ( 16 sense primers, 8 antisenset primers, 128 different primer combinations in all ) were designed from the conserved motifs glpl and cfly ( distance between them is approximately 210bp ) which existed only in the type of nbs - lrr resistance gene and used as primers for scanning the genomic dna. no specific products ( which is approximately 210bp in length and obtained from genomic dna containing ht gene ) was obtained though amplified products of approximately 500 - looobp appeared in six different primer combinations
依據nbs - lrr類r基因特有的兩個保守序列glpl和cfly (兩者相距約70個氨基酸)合成所有可能的簡並寡核苷酸引物(左引物16個,右引物8個,共組成128個引物組合)對玉米基因組dna進行擴增的結果表明:在6個引物組合中得到了擴增產物,產物長度在500 1000bp之間;沒有獲得特異性擴增產物(指僅從含ht基因材料中得到,長度約210bp的dna片斷) 。
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