細胞分裂周期 的英文怎麼說
中文拼音 [xìbāofēnlièzhōuqī]
細胞分裂周期
英文
cell division cycle- 細 : 形容詞1 (條狀物橫剖面小) thin; slender 2 (顆粒小) in small particles; fine 3 (音量小) thin ...
- 胞 : Ⅰ名詞1 (胞衣) afterbirth2 (同一個國家或民族的人) fellow countryman; compatriot Ⅱ形容詞(同胞...
- 分 : 分Ⅰ名詞1. (成分) component 2. (職責和權利的限度) what is within one's duty or rights Ⅱ同 「份」Ⅲ動詞[書面語] (料想) judge
- 裂 : 裂動詞[方言] (東西的兩部分向兩旁分開) open
- 周 : Ⅰ名詞1 (圈子; 周圍) circumference; periphery; circuit 2 (星期) week 3 [電學] (周波的簡稱) c...
- 期 : 期名詞[書面語]1. (一周年) a full year; anniversary 2. (一整月) a full month
- 細胞 : cell; sytes; bioplast; cella; [口語] gene; [生物學] cellule; cellule cellulli cellulo ; cello ; k...
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Protein kinase a is camp - dependent kinase, one of the most important signal transduction pathways, plays a pivotal role in growth, differentiation, tumor occur, cell cycle control, etc. pka activators such as camp, 8 - br - camp or phosphodiesterase ( pde ) inhibitor isomethyl butyl xanthine ( imbx ) or purified pka catalytic subunit all can inhibit germinal vesicle breakdown ( gvbd ) and meiotic maturation in mouse oocytes, also in xenopus oocytes
蛋白激酶a ( proteinkinasea , pka )是依賴于camp的絲蘇氨酸蛋白激酶,是重要的信號傳導途徑之一,廣泛參與許多生命過程,包括生長、分化、腫瘤發生、細胞凋亡、細胞周期調控等。以小鼠卵母細胞為實驗對象,給予pka激動劑camp 、 8 - br - camp或磷酸二酯酶抑制劑imbx ( isomethylbutylxanthine )均可抑制小鼠卵母細胞的胚泡破裂及減數分裂成熟。We used fission yeast schizosaccharomyces pombe ( s. pombe ), an unicellular eukaryotic organism, as research material. electroporation was adopted to load ca2 + fluorescent indicator into yeast cell and under the laser scanning confocal microscopy ( lscm ), we observed cytosolic ca2 + distribution and relative content as well as fluorescence intensity of gfp - cam in different phases of cell cycle of yeast cell. flow cytometry provided a way of determining the relative dna content of populations of fission yeast
本文以單細胞的真核模式生物裂殖酵母( schizosaccharomycespombe )為研究材料,通過激光掃描共聚焦顯微鏡觀察酵母細胞胞質內游離ca ~ ( 2 + )的分佈及相對濃度,以及不同周期時相細胞中gfp - cam的熒光強度變化,並採用細胞流式法對酵母細胞的相對dna含量進行測定以確定細胞所處周期時相。" dr. gorbsky ' s results provide elegant proof that the cell cycle must be precisely controlled, " said rodger mcever, vice president of research at the facility. " now he and his lab can work toward developing innovative methods to probe and better understand the complex process of cell division. the research is detailed in the april 13 issue of the journal nature
該基金會負責研究工作的副主席羅傑麥凱維爾說, 「戈爾博斯基博士的研究成果為下面這種觀點提供了非常有力的證據,那就是細胞分裂的循環周期必須處于精確控制之下」 , 「現在他及其所在的實驗室可以致力於開發出更具創新性的研究方法,以對細胞分裂進行進一步的探索,並使人們能夠更為深入詳細地了解細胞分裂的復雜過程」 。Wilmut said there were so many failures because it was difficult to ensure that the empty oocyst and the donor cell were at the same stage of the cell division cycle
威爾馬特說之所以有這么多的試驗失敗,是因為很難保證空卵囊和供體細胞能處在細胞分裂周期的同一階段。" our studies indicate that the factors pointing cells toward division can be turned and even reversed, " said lead researcher gary gorbsky of the oklahoma medical research foundation. " if we wait too long, however, it does n ' t work, so we know that there are multiple regulators in the cell division cycle
負責本次研究工作的加里戈爾博斯基表示: 「我們的研究表明,那些促使細胞分裂的因素是可以被改變甚至是逆轉的」 , 「我們已經知道在細胞分裂的循環周期中有多種調節因素參與其間。On the other hand, the activity and function of p27 kip1 was affected by many physiological agents such as camp, il - 2, rampamycin. the expression of p27kip1 increased after - irradiationin in the fibroblastoma cells, leading to arrest in gl phase, but its expression markedly decreased after y - irradiation in the nbcc cells, and leaded to arrest in g2 / m phase. several studies have reported that p27 kip1 involved in a much wider range of cellar processes, such as differentiation, apoptosis and migration, the expression level of p27kip1 is key for its function in dna damage responses
另一方面, p27kip 』的活性水平及功能作用,因細胞類型不同而各異,又受多種因素的影響,如一些有絲分裂原camp 、雷帕黴素可以上調p27kip ,的表達,而il一2等則與p27ki 」 『的表達下降有關,受電離輻射( ir )照射的成纖維細胞瘤及用1 , 25一二經基d3處理的hela細胞系在gl期均有游離的p27腳『的積累,細胞周期阻滯在gl期;而在痣癌伽bccs )細胞中, y一射線能誘導p27吻『表達水平的顯著下調,細胞周期阻滯在g2艦期。At that time, cytosolic fluorescence intensity decreased to normal level, which shows that most of cells get through the gl / s point and enter the log phase. when cultured in medium that neucl was omitted, most of the cells were synchronized at gl stage of cell cycle. with flow cytometry, we found that cytosolic cam content of gl cells was higher than that of normal cells at log stage
在激光掃描共聚焦顯微鏡下觀察不同周期時相裂殖酵母細胞中cam的濃度及分佈變化,結果表明,分裂期細胞總體熒光強度強于間期細胞;而對同一細胞內熒光強度的分析說明,間期細胞的熒光主要分佈於胞質中,細胞核內則分佈較少;而正在進行有絲分裂的細胞內熒光主要集中於赤道板處;剛完成有絲分裂的細胞內熒光則相對集中於兩端或其中的一端。Together, these cyclin / cdk complexes are the universal cell cycle regulators, with each complex controlling a specific transition between the subsequent phases in the cell cycle
Cyclin cdk復合物作為廣泛的影響細胞分裂調節物而發揮作用。每一種復合物參與不同的細胞周期各時段的調控。In the laboratory experiment part, human peripheral blood, cultured cells and icr mice were study objects. the changes of mitotic chromosome numbers were measured by human metaphase chromosome counts and statistic analyzed used x2 - test. the changes of meiotic chromosome numbers were measured by mice one - cell zygote chromosome counts and statistic analyzed usedx2 - test. the effects of low dose ionizing radiation on the expression of topoisomerase ii were measured by immunocytochemistry, western blot and rt - pcr
流行病學結果顯示長期小劑量輻射接觸與染色體不分離呈正相關,為進一步在細胞遺傳學和分子生物學方面研究小劑量電離輻射與染色體不分離關系及其機制,本課題第二部分以外周血、培養細胞、 icr小鼠為研究對象,用外周血染色體計數和單細胞受精卵染色體計數的方法研究小劑量輻射和拓撲異構酶復旦大學2000級博士生學位論文11a抑制劑及其二者的協同效應對有絲分裂和減數分裂染色體不分離的影響,用免疫細胞化學染色、 westernblot 、 rt pcr等方法研究了電離輻射引起拓撲異構酶a表達變化。With the results of analysis of cells morphology and cell cycle determination, c. chinensis extract, inhibiting pc 12 cells proliferation, inducing neurite outgrowth, was testified. at the same time, c. chinensis extract can also amplify expression of neuroregenerating maker molecule, growth associated protein - 43, increase the activity of acetylcholinesterase in rat pheochromocytoma pc 12 cells
菟絲子提取物處理后的pc12細胞形態分析、免疫組化和細胞周期測定結果表明,菟絲子提取物不僅能抑制細胞分裂,誘導細胞分化、延伸大量突起,而且能促進神經元再生信號蛋白gap - 43的大量表達,提高乙酰膽堿脂酶( acetylcholinesterase , ache )的活性。In addition, the immediate elf - emf exposure might induce cell cycle arrest, thus also contributing to its stimulating effect of neurogenesis from mesencephalic neural progenitors
此外, elf emfs抑制中腦神經前體細胞增殖,加速神經前體細胞退出細胞分裂周期,可能也是elfThe results show that c. chinensis extract can prevent pc 12 cells apoptosis induced by reactive oxygen species ( ros ), intensify cells viability, inhibit proteins expression related to apoptosis, regulate cell cycle, increase the level of sod and gsh, and decrease the product of mda
它不僅能提高細胞活力,調節細胞中凋亡相關基因的表達,而且還能調控細胞分裂周期,提高神經分化的pc12細胞中sod和gsh的含量,降低mda水平。Mpf ( m phase promoting factor ) which is made up of cdc2 as the active subunit and cyclin b as the regular subunit is a key factor in cell division. cdc2 is the active part of the mpf, which can active multiple ser / thr
Mpf ( mphasepromotingfactor )是細胞分裂的中心調控因子,由催化亞基cdc2和調節亞基周期素b ( cyclinb )組成,其中cdc2是mpf的活性單位,具有絲蘇氨酸蛋白激酶活性。Rapid responses occured when cells were exposed to genotoxic stresses : one is initiating dna damage checkpoint that leads to arrest in gl, in order to provide the cell with sufficient time to repair damaged dna, or leads to arrest in g2, in order to avoid defective chromosome happening mitosis. on the other hand, if damage to the genome is excessive, the cells initiating apoptosis, when damages cannot be repaired
這是因為細胞在受到諸如電離輻射等基因毒應激損傷時,細胞最先作出的細胞學反應:一,起始dnadamagecheckpoint使細胞周期阻滯( arrest )在g1期,贏得時間識別與修復損傷,避免損傷的dna進行錯誤的復制,或和阻滯在g2期,避免缺陷的染色體進一步分裂;二,損傷嚴重而修復無望啟動細胞死亡程序,清除那些帶有病變傾向的細胞危害機體。B - actin primers : forward 5tcc tat cgg cct cct cct ac 3 " and reverse 5aga atg tag tcc gcc agg tc 3 ", designed for 200bps. saodn oligodeoxynucleotide of pkb : stac agc ctg aga gga cct acc tg3 "
在有絲分裂期, edc25經歷216位ser磷酸化同時其水解酶活性增強,活化的mpf磷酸化並活化edc25 ,形成正反饋,結果進一步活化mpf前體,活化的mpf促進細胞周期g2 / m期轉換。The onset of mitosis is induced by activation of mpf, a highly conserved complex consisting of a kinase, cdc2, and an activating subunit, cyclin b. during the cell cycle the concentration of the catalytic subunit cdc2 remains constant while cyclin accumulates from interphase to mitosis and is degraded at each metaphase - anaphase transition
在許多物種的早期胚胎細胞分裂過程中,由間期到m期的連續分裂是一個重要特點。 mpf ( m期促進因子, m - phasepromotingfactor , mpf ,又可稱為成熟促進因子maturationpromotingfactor , mpf )由cdc2和周期素b兩個亞基組成。The precise control of the key checkpoints of the cell cycle, such as g1 / s, s / g2, g2 / m and mitosis exit, ensures the eukaryotic cells to proliferate and divide in an orderly and programmed manner
對g1 s , s g2 , g2 m以及走出m期各轉換點的調控,保證了細胞周期各事件按次序正常發生,是細胞正常分裂、增殖和生長的保障。分享友人