細胞多核化 的英文怎麼說
中文拼音 [xìbāoduōhéhuà]
細胞多核化
英文
multinucleation-
The study of astragalus polysaccharides on inducing the cord blood monocyte into the dendritic cells
黃芪多糖誘導臍血單核細胞分化為樹突狀細胞的實驗研究Cytologic examination shows benign appearing spindle cells, chondroid cells, multinucleated giant cells and calcified debris ( diagn cytopathol 2001 ; 24 : 336 )
細胞學檢查顯示良性的梭形細胞、軟骨樣細胞、多核巨細胞以及鈣化的碎屑。The steady dead generation and time that was caused by the isolated virus was certain by chicken embryo which was inoculated on seven or nine days. the histopathological changs of the infectious stunting syndrom were studied by the way of ordinary paraffin section and he dying. the experimental result were as follows : the test proved that the changes of the chicken embryo were different in different stage. the chicken embryo dead in a week after it inoculated. the body was dropsy and hemorrhage. dead before it hatched out, the embyo body were dropsy, pale and slime. the liver was yellow and swolled, gallbladder ( vesica fellea ) was filled with bile. bursa and glandula thymus analosis. the kindey dropsy. bowel lamina were humble, dilatation. gas and yellow foam were filled the bowel. histopathological changes were that, in early stage, obvious changes of liver and kindey were dropsy, hemorrhage and necrosis. two types eosinophilic intranuclear inclusion bodies including large round and little granular were present in cells of the above organs. the obvious changes of bursa were dropsy, adverse folliiculated growth and little lymphocytes proliferating, 19 - 21 days chicken embryo, one or two big empty vacuoles were prensent in cells of liver and kindey. the number of the folliculi was growing, the vacuoles between cells were larger
膽囊充盈、其內充滿稀薄的膽汁；法氏囊、胸腺萎縮，腸道擴張、腸壁菲薄、內充滿氣體及黃色泡沫狀物；腎臟腫大。病理組織學變化方面，早期肝臟、腎臟、腸主要以出血、水腫和壞死為主，且肝細胞核及腎小管的上皮細胞核內均發現有核內包涵體，包涵體呈嗜酸性，為大型圓形包涵體或不規則的顆粒狀；法氏囊則以水腫、濾泡發育不良、小型淋巴細胞數量增多為主。 19 21日齡雞胚肝細胞、腎小管上皮細胞的胞漿內出現1 2各大的空泡，法氏囊濾泡數目增多細胞間有較大空隙。Methods : hyperosmotic pressure animal model was established by administering 3 % sodium chloride as drinking water to rats or increasing osmotic pressure of the culture medium. osmoregulation positions in the brain, reciprocal projection pathways between the medullary visceral zone ( mvz ) and supraoptic nucleus ( son ) or hypothalamic paraventricular nucleus ( pvn ), oscillation of intracellular calcium in cultured neurons and astrocytes were studied by means of anti - fos, glial fibrillary acidic protein ( gfap ), tyrosine hydroxylase ( th ) or vasopressin ( vp ) multiple imrnunohistochemical staining, immuno - electronic microscope, wga - hrp retrogradely tracing and cell culture methods. results : ( 1 ) fos positive neurons within the mvz, parabrachial nuclei, locus ceruleus, pvn, son, subfomical organ increased markedly
方法：通過給予大鼠飲用3氯化鈉或提高培養基滲透壓濃度的方法復制高滲刺激模型，主要採用抗fos 、膠質原纖維酸性蛋白（ gfap ）和酪氨酸羥化酶（ th ） （或加壓素? vp ）免疫組織化學多重染色、免疫電鏡、 wga - hrp束路追蹤結合免疫組織化學多重染色、細胞培養等實驗方法，系統觀察了中樞參與滲透壓反射的調控部位、下丘腦視上核（ son ）神經元? ast超微結構的變化、延髓內臟帶（ mvz ）和son及下丘腦室旁核（ pvn ）之間往返投射通路和神經元的性質及其與ast的關系、培養神經元和ast內鈣波的變化。Fos + / th + / gfap + and fos + / vp + / gfap + triple labeled n - asc could be found in the mvz, pvn and son respectively ; ( 2 ) under electronic microscope, the astrocytic processes connected closely with the dendrites or axons of the neurons, where the bilateral membranes became thick. we call transiently it electron - dense areas ( edas ). the number of edas increased remarkably following hyperosmotic stimulation ; ( 3 ) when trace retrogradely, wga - hrp was microinjected into the unilateral son, pvn or nucleus of solitary tract ( nts ) respectively using the stereotaxic method, the n - ascs formed by the neurons triple - labeled with hrp / fos / th ( or vp ) and astrocytes labeled with gfap could be found in the mvz, son and pvn respectively ; ( 4 ) after being treated with heperosmotic nacl solution, intracellular calcium concentration in cultured hypothamic neurons and astrocytes increased and then decreased
腦內gfap陽性結構也明顯增多，其分佈與fos陽性細胞分佈基本一致，表現為胞體肥大、突起粗長； ast緊密包繞在神經元周圍形成神經元- ast復合體（ n - asc ） ；在mvz 、 pvn和son三重免疫組化染色切片上可見到fos + th + gfap +第四軍醫大學博士學位論文和fos vp gfap三重標記asc ； （ 2 ）免疫電鏡下son內星型膠質細胞突起與神經元樹突或軸突之間接觸部位出現增厚的膜結構一電于緻密區（ edas ） ，高滲刺激后數量明顯增多： （ 3 ）將們個mp注入大鼠一側n卜、卜卜或孤束核（ ws ） ，分別在延髓內臟帶（ mvz ） 、 so和pvn內出現fos hrp th 、 fos hrp八p三重標記神經元和gfap陽性標記ast形成的n asc ； （ 4 ）高滲刺激使培養神經元和ast內鈣水平先升高后降低，最後維持在比高滲刺激前稍高的靜息鈣水平上。Primary culture of rat preadipocyte were prepared from the epididymal, inguinal and perirenal the fat pads of male normal, healthy, 15 - 20 days sprague - dawley rats. the preadipocyte grew better under the condition of 37, 95 % humidity, 5 % co2, ph 7. 0 - 7. 2, centrifuged at 1000r / min, m199medium, and 10 % fetal bo vine serum, seeded at a density of 4 l04, 5 l04, / cm2. oil red o staining was the special method to distinguish adipocyte from other cells, gimsa and he could determine the stage of the adiopcyte differentiation through the number of lipid drop, size and the position of the nucleolus of the staining fat cell
經過多次實驗，確定本實驗室大鼠前體脂肪細胞的最佳培養條件是：溫度為37 ，濕度為95 ， co _ 2濃度為5 ， ph值為7 . 0 7 . 2 ，離心力為1000r / min ，培養基為m _ （ 199 ）培養基，胎牛血清濃度為10 ，合適細胞接種密度為4 10 ~ 4 、 5 10 ~ 4個/ cm ~ 2 ，染色結果表明：油紅o染色是鑒定脂肪細胞的特異方法， gimsa和he染色可根據不同區域染色程度、著色差別判斷細胞核的位置及脂滴大小、多少，觀察大鼠前體脂肪細胞分化過程中的形態變化，進而確定脂肪細胞的分化階段。. from the direct mutant of spirulina platensis ( sp - d ), we got high purity and activity phycobiliprotein which could grow crystals. the algae fluorescent probe prepared by coupling the above polyclonal antibody to phycobilipotein not only keeps the property of stronger anti - fluorescence quenching but also has the lower fluorescent background when it was used for labeling stoma cells of pea tendril
以原核表達的peac1為抗原制備了免疫活性較好的抗豌豆肌動蛋白的多克隆抗體，從螺旋藻中純化了高純度、高活性、能結晶的藻膽蛋白，將兩者偶聯制備的藻熒光探針，不僅保持了藻膽蛋白很強的抗熒光淬滅能力，而且用於豌豆卷須氣孔細胞熒光標記時有更低的熒光背景。In this study, the expression of nfkb p65 and ikba ( inhibitory kappa b alpha, ikba ) in poly - morphonnucleared cells, mononuclear cells and fibroblastic cells during rat skin contusion repair will be investigated using immunohistochemical technique and the relationship with contusion will be revealed, which can provide the theorical evidence to identify the contusion age
I b因其抑制nf b活性，也越來越受到人們的重視，故本實驗應用免疫組織化學方法觀察nf b家族的重要成員nf bp65與其抑制因子i b在大鼠皮膚挫傷后不同時間在多核粒細胞、單核巨噬細胞及成纖維細胞中的表達變化，揭示其與皮膚挫傷時間的關系，為皮膚挫傷時間的判定提供新的理論依據。Estrogen levels are high, there are more smear diversification cells, nuclear deep into dense ; low levels of estrogen, smear a small round or oval, blue - stained nuclear osteoporosis bottom of the cell
雌激素水平高時，塗片中有較多角化細胞，核緻密深染；雌激素水平低時，塗片中出現小而圓或卵圓形，核疏鬆藍染的底層細胞。In young chickens aev induces paralysis, ataxia and muscular dystrophy, while in older chickens, infection is usually subclinical, resulting in a decline in egg production and hatchability. infectivity was shown to remain unaffected by chloroform, low ph, pepsin, trypsin and deoxyribonuclease. magnesium cations were shown to stabilise preparations of the virus against heat inactivation. the buoyant density of virions are 1. 31g / ml. the diameter of the virion was estimated to be 22 to 30nm. the aev can be adapted to grow in chicken embryo. the inability of aev to grow effeciently in most cell cultures
幼雞感染該病毒后，引起麻痹、頭頸震顫甚至共濟失調，而成雞常呈亞臨床感染或導致產蛋量和孵化率下降。病毒的感染性不受氯仿、低ph 、胃蛋白酶、胰酶和脫氧核糖核酸酶的影響，鎂離子可增強病毒對熱的穩定性，病毒的浮密度為1 . 31g ml ，直徑為22 - 30nm ，該病毒主要在雞胚中增殖，在大多數細胞培養物中不生長。Nucleoli exist from spermatocyte stage to early spermatid stage. in this process, the numbers of ribosomes, mitochondrions and endoplasmic reticulums increase until secondary spermatocyte stage, golgi apparatus emerges at the secondary spermatocyte stage, and then, these organelles change into lamellar complex which finally forms the acrosome
核糖體，線粒體和內質網等細胞器數量逐漸增多，到次級精母細胞階段達到最大，在形成精子的過程中，上述細胞器與高爾基體及膜性泡共同分化或參與形成片層小體，並參與頂體的形成。Mutated plasmid was transformed into e. coli tg1 cells to produce engineered peptide, then the peptide was purified by cm sepharose ion - exchange column. in vitro bactericidal assay and drug withdrawal were used to identify the bioactivity of the engineered peptide. the planar lipid bilayer membrane was used to assay the electrophysiology of the engineered peptide. toxicity studies on mammalian cells were used to assay the toxicity of the engineered peptide
將重組質粒轉化入大腸桿菌tgi工程菌中，生產構建的工程多膚，離子交換純化后獲得工程多膚初步純化產物，體外抗菌試驗、藥物撤離試驗檢測工程多膚的抗菌活性，在人工脂質膜上測定其形成離子通道的特性以初步研究抗菌機理， ?並觀察其對真核細胞的毒性作用。In this paper, the effect of nuclear actin on the process of chromosome construction has been studied by utilizing the precise natural synchrous plasmodium of physarum polycephalum, sds - polyacryl amide gel electrophoresis ( sds - page ), western blotting, the cell - free system and optics microscopy. the major results and conclusions are as follows : 1
本實驗以多頭絨泡菌原質團為材料，採用同步化培養、細胞核提取、 sds - page 、免疫印跡、非細胞體系構建、光學顯微鏡觀察等方法，研究了有絲分裂前期核內肌動蛋白對染色體構建的影響。It distributes widely and survives easily. smears of blood samples were prepared conveniently ; and there is no restriction on seasons. for these reasons, mcn and ona test in erythrocytes of loach is rather useful index for detecting mutagenetic compounds in the aquatic environment
研究結果表明，泥鰍對于核變異的誘發具有較高的敏感性，且泥鰍分佈較廣，易存活，且血量也較搬料多，並能在不同季節評測水質狀況，因此，泥鰍紅細胞微核和核異常測試法可作為監測水環境中致變化合物的一個較為實用的指標。The first biological effect of ionising radiation is the absorption of energy, leading to the removal of electrons from atoms. this occurs almost instantaneously and is followed by chemical damage to cells, which can include damage to dna
第一，電離輻射能吸收能量，令電子脫離原子，這過程差不多即時發生，令細胞因化學反應而受損，包括脫氧核糖核酸dna 。The stage when the block occurs has been correlated to the time the embryonic genome takes over control of development from the maternal genome, which occurs in mouse at the 2 - cell stage. the embryos of balb / cxicr fl mouse show 2 - cell block in vitro and the effects of several culture media on overcoming the 2 - cell block in embryos development were studied. m16 ( sigma ), in which 96. 2 % of 1 - cell embryos developed to 4 - cell stage, were shown to be effective in overcoming the 2 - cell block
培養5h后， 53去核卵母細胞可見標記的mtocs ，但數量明顯少於對照組，而又多於培養0h的去核細胞，說明此時53的化學去核卵胞質中確有mtocs的存在，其微管聚合能力正在恢復； mtocs標記，但其輻射產生的微管纖維短，結構還不夠豐滿；其餘細胞的微管蛋白則喪失重新裝配能力。Based on poor glandular formation, necrosis, cellular atypia, nuclear pleomorphism, invasion of soft tissue and bone, and vascular permeation
主要基於腺管的結構分化不良，壞死，細胞異型性，核多型性，軟組織和骨浸潤以及血管浸潤。Embryonic stem ( es ) cells are pluripotent cells derived from the inner cell mass ( icm ) of early blastrocyts, which possess the ability of unlimitted proliferation and maintain normal dipoid keptypes under differentiation - inhibited culture in vitro, they will differentiate into multiple lineage cell types or occur commentent and differentiation without differentiation - inhibited factors or hi presence of certain induced - factors.
胚胎幹細胞（ es細胞） ，是存在於早期胚胎內細胞團（ icm ）的一種多潛能細胞。在體外抑制分化培養時， es細胞可無限增殖並保持正常的二倍體核型，一旦撤除分化抑制因素，或添加適當誘導劑， es細胞可發生譜系分化或定向分化。The activity of the antiserum was tested by dot enzyme immunization assay ( diba ) with purified antigen. western blot of hela nuclear protein extract, which contained natural hbaf53, showed that the antiserum is also a specific antiserum to natural hbaf53. therefore the highly specific and sensitive antiserum can be applied to various studies
用純化的抗原蛋白，經斑點印跡試驗測得baf53抗血清的效價，又用提取的hela細胞核蛋白（含有天然baf53蛋白）進行免疫印跡分析（ westernblotting ） ，證明天然baf53蛋白也是該抗血清的抗原，說明獲得的多抗血清具有高特異性和敏感性，可用於多方面的研究。Sequence analysis shows that they share 98. 75 % similarity at the dna, and 98. 67 % at the protein level. ns2 gene was cloned into the multiple cloning sites of prokaryotic expression vector pgex - 6p - l. the recombinant plasmid pgex - 6p - ns2 was constructed and transformed to the competent cell bl21 ( de3 ) plyss, positive bacterium strain was induced by iptg
將ns2基因插入到原核表達性質粒pgex - 6p - 1的ecor 、 bamh多克隆位點之間，將重組原核表達質粒pgex - 6p - ns2轉化到bl21 （ de3 ） plyss感受態細胞中，獲得了表達ns2基因的陽性亞克隆重組子，在含amp的lb液體培養基中培養，經iptg誘導表達，用sds - page分析表達產物。分享友人