翻譯移碼 的英文怎麼說
中文拼音 [fānyìyímǎ]
翻譯移碼
英文
translation frameshift- 翻 : 動詞1 (變換位置; 歪倒; 反轉) turn over; turn around; reverse 2 (移動物體尋找) rummage; search...
- 譯 : 動詞(翻譯) translate; interpret
- 移 : Ⅰ動詞1. (移動) move; remove; shift 2. (改變; 變動) change; alter Ⅱ名詞(姓氏) a surname
- 碼 : Ⅰ名詞(表示數目的符號或用具) a sign or object indicating number; code Ⅱ量詞1 (指一件事或一類的...
- 翻譯 : 1 (把一種語言譯成另一種語言) translate; interpret; put into ; turn into ; transcribe; render 2 ...
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This expression vector plbcas - hsa - lgl has the following advantages : i ) the 1. 7kb promoter is able to drive cell - specific and hormone - dependent expression ; ii ) the inclusion of intron - 1 can increase expression level of fusion genes ; iii ) the 5 ' utr of bovine p - casein mrna may have a positive role in both transcriptional and post - transcriptional regulation ; iv ) the gfp gene make the selection of positive clone among embryos possible ; v ) the gfp gene can be easily excised via cre - mediated recombination between the two loxp sites after the expression vector has been integrated into chromosome ; vi ) the two incompatible lox sites, loxp and lox2272, would facilitate cre - recombinase mediated cassette exchange ( rmce ), which in theory will leading to develop a technology of site - specific gene expression in animal mammary glands
該載體的特點是:具有可以調控外源基因在乳腺中特異表達的牛-酪蛋白基因5 `端側翼區和包括第一外顯子及內含子在內的5 `端調控區;將人血清白蛋白cdna準確地置於牛-酪蛋白基因第二外顯子中的翻譯起始密碼子atg之後,而且沒有增加額外的序列和使人血清白蛋白cdna移碼;引入標記基因gfp ,便於在胚胎期鑒定陽性胚胎,減少受體;引入cre lox重組系統: ( ? )標記基因gfp的兩端的兩個loxp位點可以在表達載體整合到基因組后,刪除標記基因; ( ? )餘下的一個loxp位點可以和前面的lox2272位點組成cre重組酶介導的盒式交換系統。Binary translation is an important technique to resolve the problem of code migration
二進制翻譯技術是用軟體方法解決代碼移植問題的重要手段。A new e. coli promoter - probe vector phn1005 was constructed by using a red - shift enhanced gfpmut3 as reporter gene and showed the following characters : 1, bamhi at the 5 " end of gfpmut3 structure gene could be used to clone promoter - active fragment and the strength of promoter could be quantitatively assayed. 2, a rrnbtlt2 terminator from rrna gene at the 3 " end of gfpmut3 could permit clone strong promoter. 3, another rrnbt1t2 terminator was inserted upstream bamhi to prevent reading through of promoters from puc19
進一步以紅移且熒光強度提高21倍的gfpmut3為報告基因,構建了大腸桿菌啟動子探針載體phn1005 ,該載體上gfpmut3結構基因5 』端的bamhi位點可用來克隆具有啟動子活性的dna片段並定量分析插入的啟動子強度;其3 』端含rrnat1t2終止子,可允許克隆強啟動子;在bamhi上游同樣插入rrnat1t2終止子以防止載體puc19上的啟動子的轉錄通讀; gfpmut3結構基因上游還插入一段內含子序列使正反六種讀碼框的翻譯均可被終止,可保證gfpmut3以正確的讀碼框翻譯。The research on the binary translation and the related optimization has signality not only for legacy code migration but also for the program performance improvement and other aspect
二進制翻譯及相關優化技術的研究,不僅在遺產代碼移植而且在程序性能提高等其它方面都有重要的意義。分享友人