蹄行性 的英文怎麼說
中文拼音 [tíhángxìng]
蹄行性
英文
unguligrade-
The practical issue of drum brake with oversized overall stroke was resolved by experiments, and it indicates that major effect factors of air chamber overall stroke, which are the drum - shoe clearance, the elastic modulus of lining, the torsion deflection of cam shaft
研究結果表明，氣室推桿總行程過大的主要影響因素為制動蹄與制動鼓之間的間隙、摩擦片的彈性模量、凸輪軸的扭轉變形。In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。Selective microsurgical tibial neurotomy combined with subcutaneous achilles tendon slipping andlengthening for the treatment of spastic cerebral palsy with equines
選擇性脛神經肌支切斷加跟腱皮下滑行延長術治療腦癱馬蹄足痙攣This paper gives a brief introduction of the formation mechanism of sma and the handing property of pavement from the aspects of theory and research. at the same time, this paper also studies the effect of aggregate size on road - related performance in some different grads. except, it also having inspected to use the sbs, the pe and the combination material of sbs and pe to analyze the influence to the function of material road
瀝青瑪蹄脂碎石混合料（ sma ）以其溫度穩定性好、抗滑性能優良、低噪音、使用耐久等優點在公路建設中越來越受到重視，本文對sma組成特點，強度形成機理與路面使用性能從理論和試驗研究角度進行論述，並針對不同級配的瀝青瑪蹄脂碎石混合料，分析了粗、細集料的粒徑變化對其路用性能的影響，同時也考察了用sbs 、 pe 、 sbs和pe復合改性后的瀝青結合料對混合料的路用性能影響。These can make up the shortcoming of slrcp ; 2 3. to study the steel stress development of penstock, ring - form slsfsrcp and horse - shoe slsfsrcp during curing period are monitored. the pre - tension stress due to expansion of self - stressing concrete can be used in design ; 4
對圓形和馬蹄形管道進行了28天養護期內力監測，研究了鋼襯鋼筋鋼纖維自應力混凝土壓力管道的自應力成長過程，明確了鋼筋的預拉應力數值，為此類改性管道設計直接提供依據； 4Based on the chang - yu highway in julin province, this paper focus on gap - graded asphalt mixtures. aggregate grading of the gap - graded hma was designed by means of void design procedure. this paper give out a kind of new gradation of stone matrix asphalt. at the same time, this paper also studies the effect of aggregate size on road - related performance in some different gradings
本文以吉林省長余高速公路為依託，從瀝青混合料的結構形態入手，利用體積法，對骨架密實型級配類型的瀝青混合料的級配進行研究，提出新型的瀝青馬蹄脂級配，並針對不同級配的瀝青馬蹄脂碎石混合料，全面分析了粗，細集料的粒徑變化對其各種路用性能的影響。The global bifurcation analysis of the nonlinear nonplanar cantilever is given by a global perturbation method developed by kovacic and wiggins. it is found that the nonlinear nonplanar cantilever can undergo the hopf bifurcation, heteroclinic bifurcations and silnikov - type homoclinic orbit to saddle focus, which means that the nonlinear nonplanar cantilever can give rise to the chaotic motion in the sense of smale horseshoes
利用kovacic和wiggins的全局攝動法對非線性非平面運動懸臂梁進行了全局動力學分析，發現系統存在hopf分叉和異宿分叉，並證明系統有silnikov型鞍焦點型同宿軌道，可以產生smale馬蹄意義下的混沌。In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals
首先，用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上，並轉化e colidh5a感受態細胞中，經pcr 、酶切以及測序證明得到了完整的2c3abc基因，並與國內外參考序列進行比較分析。然後，將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ，以zeocin ~ （ tm ）抗性篩選陽性克隆，大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞，通過zeocin ~ （ tm ）抗生素梯度濃度篩選，獲得重組酵母用0 . 5甲醇誘導表達，通過sds - page電泳、 westernblotting分析，結果表明， 2c3abc基因在畢赤酵母中成功表達，其表達產物為一95ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。分享友人