酶轉化 的英文怎麼說

中文拼音 [zhuǎnhuà]
酶轉化 英文
enzymatic conversion
  • 酶 : 名詞[生物化學] （生物體的細胞產生的有機膠狀物質） enzyme; ferment
  • 轉 : 轉構詞成分。
  • 轉化 : 1. （轉變） change; transform 2. [化學] inversion; conversion
  1. Transketolase is the key enzyme of pentose - phophate pathway, catalyses transfer of a two - carbon fragment from a ketolase ( donor substance ) to an aldose ( acceptor substance )

    摘要轉酮醇酶是磷酸戊糖途徑的關鍵酶，催化二碳單元在酮糖（供體）和醛糖（受體）間的轉移。
  2. Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method

    利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
  3. Screening target ' gene of artemisinin antimalarials using drug - western from cdna expressing library : 12 b - deoxoartemisinyl - ( 4 ' - oxyacetic acid ) phenyl ether was linked to bsa by using of edc cross ! inker and the product acted as drug - probe

    對重組pmd一18一t克隆載體及pqe一30表達載體雙酶切，提取tctp基因和pqe一30空載體並使二者重組，然後轉化m15 ，挑取陽
  4. In this study, the stem segments of new shoot with axillary buds of well - growth tetraploid black locust trees were used as explants. the effects of different basic mediums, different hormone kinds and their concentrations ratios, different sucrose concentrations on calli induction, buds differentiation and rooting in the process of establishment of high frequency regeneration system of tetraploid black locust were studied. on the base of high frequency regeneration system, the effects of various factors on transformation efficiency of badh mediated by agrobacterium tumefaciens were discussed in the light of gus histochemical assays

    本實驗首先以生長良好的四倍體刺槐優株上當年生新梢的帶腋芽莖段為外植體，研究了在四倍體刺槐高頻再生體系的建立過程中不同基本培養基、不同激素濃度及其配比、不同蔗糖濃度對愈傷組織的誘導、芽的分化及生根的影響；然後在得到高頻再生體系的基礎上，通過農桿菌介導法轉化甜菜堿醛脫氫酶（ badh ）基因，以gus染色組織分析為依據探討了影響轉化效率的各種因素，建立了高效、可重復的基因轉化體系，為四倍體刺槐目的基因的導入打下了基礎。
  5. E. coli xl1 - blue cells were tansformed by psurfpga and phages were rescued by m13ko7 helper phage particles. results showed that the heterodimeric enzyme was expressed as a fusion protein that matures to an active biocatalyst connected to the coat protein of phage fd

    以構建的噬菌粒psurfpga轉化具有琥珀突變的大腸桿菌xl1 - blue ，以輔助噬菌體m13k07超感染，進行青霉素g酰化酶基因的表達和在噬菌體表面的展示。
  6. Biotransformation in organic solvent is an attractived field in nowadays. compared with isolated enzyme whole cell is not used broadly as biocatalyst. in this research the cells of baker ' s yeast is adopted to mediated a model reaction in organic solvent, in which geraniol is converted to citronellol reductively

    有機相生物轉化是當前生物技術中一個具有理論研究意義和應用價值的領域，目前該領域的研究大多集中在利用分離的酶進行生物催化，利用微生物完整細胞進行的研究比較少。
  7. Thrombin is an enzyme that catalyzes the conversion of the soluble plasma protein fibrinogen to the insoluble protein fibrin.

    凝血酶能催化可溶性血漿纖維蛋白轉化為不溶性的纖維蛋白。
  8. Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed

    本實驗以河套蜜瓜果肉基因組dna為模板，用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增，得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上，篩選反向克隆，然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游，構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上，通過花粉管通道法轉化河套蜜瓜，共獲76顆瓜，並進行了硬度和含糖量的分析。
  9. Decarboxylase an enzyme that catalyzes the decarboxylation of carboxylic acids, including the conversion of amino acids to amines

    脫羧酶：能夠催化羧酸進行脫羧反應的酶，包括氨基酸轉化為胺的反應。
  10. Alcohol dehydrogenase an enzyme that converts ethanol into acetaldehyde ( ethanal )

    醇脫氫酶：把乙醇轉化為乙醛的酶。
  11. A double - strand cdna fragment of dh ( diapause hormone ) gene was obtained from kt - pcr amplification. the fragment was digested by two restriction enzyme nde 1 / ecor 1 and then was joined with pet - 30a ( + ) plasmid which was digested by nde 1 / ecor 1 too. then the outcome was transformed into escherichia coli bl21

    Pcr產物經nde / ecor雙酶切后，與同樣雙酶切的pet - 30a （ + ）質粒連接並轉化至大腸桿菌（ escherichiacoli ） bl21中，經檢測篩選，成功得到陽性克隆。
  12. Study of the transport of small molecules in a microemulsion - based organogel is of great significance to broaden the research area of micellar enzymology and to promote its application in biosynthesis, bio - transformation and biosensor

    微乳凝膠中小分子傳質研究對于拓寬膠束酶學研究內涵、加速酶在生物合成與轉化領域中的應用、研究高性能生物傳感器等具有重要理論意義和潛在應用價值。
  13. The potential for a wide range of biotransformations using either immobilized cells or extracted plant enzymes undoubtedly exists, the major restriction to development being our lack of fundamental knowledge of the general biochemistry and enzymology of natural product synthesis

    盡管我們在自然產品合成方面的普通生物化學和酶學知識還很匱乏，但使用固定化細胞或植物提取酶的生物轉化得到大范圍應用的可能性無疑是存在的。
  14. Enzymatic assay were performed using recombinant proteins expressed in e. coli cells. tilapia 17 - hsd1 is nadp ( h ) dependent and it can catalyze the interconversion from estrone to estradiol efficiently and also from androstendion to testosterone but less efficiently

    酶活性實驗結果顯示，在e . coli細胞中表達的17p一hsdi / petbluez重組蛋白既能高效地催化a和t之間的相互轉化，同樣又能更高效地催化e1和ez之間的相互轉化。
  15. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  16. The principle and method for enzymatic synthesis of gallic acid, isolation and selection of the aspergillus niger strains, characteristics of this biotechnology, products quality of gallic acid and the uses in domestic food and pharmaceutical industries are briefly introduced

    摘要概述了黑麴黴單寧酶酶法轉化五倍子單寧酸生產沒食子酸的原理和方法、酶源菌種的分離和選育、工藝技術的特點、產品質量規格及在國內食品、醫藥行業相關部門的應用等。
  17. For example, in glycolysis, glucose phosphate isomerase catalyzes the conversion of glucose 6 - phosphate to fructose 6 - phosphate

    例如，在糖酵解過程中葡糖磷酸異構酶催化6 -磷酸葡糖轉化成為6 -磷酸果糖。
  18. The quality of an enzyme immunoassays depends very much on the purity of the antigen or hapten used for conjugation, the specificity of the antibody and the choice of a suitable enzyme label

    酶免疫分析技術的質量依賴于抗原的純度、抗體的特異性、合適標記酶的選用，其靈敏度取決于標記酶的高度純化和高轉化率。
  19. The number of 3 rhizosphere microorganisms ( bacteria, fungi, actinomycete ) and 5 enzyme ( catalase, protease, urease, phosphatase, invertase ) activities were studied during the whole life of corn plant in sandy loam, loam and clay soil textures. [ method ] using yedan22, the number of 3 rhizosphere microorganisms and 5 enzyme activities with different textural soils were investigated in a pond

    摘要目的明確不同質地土壤（砂壤、中壤、重壤）玉米生育期間根際微生物（細菌、放線菌、真菌）數量與酶（脲酶、蛋白酶、磷酸酶、轉化酶、過氧化氫酶）活性的變化。
  20. [ objective ] the number of 3 rhizosphere microorganisms ( bacteria, fungi, actinomycete ) and 5 enzyme ( catalase, protease, urease, phosphatase, invertase ) activities were studied during the whole life of corn plant in sandy loam, loam and clay soil textures. [ method ] using yedan22, the number of 3 rhizosphere microorganisms and 5 enzyme activities with different textural soils were investigated in a pond

    摘要目的明確不同質地土壤（砂壤、中壤、重壤）玉米生育期間根際微生物（細菌、放線菌、真菌）數量與酶（脲酶、蛋白酶、磷酸酶、轉化酶、過氧化氫酶）活性的變化。
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