閱讀框架 的英文怎麼說

中文拼音 [yuèdòukuàngjià]
閱讀框架 英文
open-reading frame
  • 閱 : 動詞1. （看） read; go over 2. （檢閱） review; inspect 3. （經歷; 經過） experience; pass through
  • 讀 : 讀名詞（語句中的停頓） a slight pause in reading
  • 框 : 框名詞（框架; 框子） frame; case
  • 架 : Ⅰ名詞1 （用來放置東西或支撐物體等的東西; 架子） frame; rack; shelf; stand 2 （毆打; 爭吵） fight;...
  • 閱讀 : read
  • 框架 : [建築] frame; framing; shell frame; skeleton frame; frame mount; trellis; sash; pigsty（e）; rack...
  1. The pcr product was inserted into expression plasmid pet - 32a ( + ) after restriction digest. then the recombinant plasmid was identified by endonuclease analysis, pcr ampliation and dna sequencing. the report showed that the recombinant plasmid had right open reading frame

    重組質粒經酶切鑒定， pcr鑒定和測序，結果證實豬肺炎支原體黏附因子p97基因的抗原決定簇r1區定向插入了質粒pet - 32a （ + ） ，且閱讀框架正確。
  2. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  3. We conclude that the gene we studied is a new loci required for efficient nitrogen fixation and for competitive nodulation of soybeans by bradyrhizobium japonicum strain gx201

    我們認為在突變體菌株gx217中tn5gusa5所插入的開放閱讀框架是一個新的與競爭結瘤有關的基因。
  4. Orf, open reading frame

    和開放閱讀框架
  5. The results of sequencing showed that jl94 isolate complete gene 6 was 1356bp and had a complete open reading frame which encoded 397 amino acides

    對克隆的vp6基因進行序列測定，測序結果顯示jl94vp6基因全長1356bp ，含有完整的開放閱讀框架，編碼397個氨基酸。
  6. This sequence emergences fourteen times from 1000 ests library indicts that it is a middle affluently gene in cdna library. the cdna of 634 basepairs contains an open reading frame of 339 nucleotides encoding a novel nonspecific lipid transfer protein. the first 23 amino acids constitute the putative signal peptide, characteristic for targeting to the secretory pathway

    測得th - nsltp序列全長為634bp ，含有一個非特異性脂轉移蛋白與植物耐逆性的相關性研究編碼112個氨基酸的閱讀框架， n端的23個氨基酸組成一段信號肽序列，表明它可能和分泌有關。
  7. Badh cdna ( 1901bp ) included a 66 bp 5 " utr, a 329 bp 3 " utr and a 1506 bp orf encoding a 501 - ammo - acid polypeptide which showed 88 % sequence identity to badh from spinach, sugar beet and atriplex hortensis respectively. the deduced amino acid sequence included a decapeptide sequence " vtlelggksp ", which is highly conserved among general aldehyde dehydrogenases ( aldh ), and a cysteine residue

    Badhcdna全長1901bp ， 5端非編碼區66bp ， 3端非編碼區329bp ，含有2個可能的加polya信號： aataa ，開放閱讀框架1506bp ，編碼一個由501個氨基酸構成的多肽，與菠菜、甜菜、山菠菜badh的氨基酸序列同源性均為88 ，其中有醛脫氫酶的保守序列vtlelggksp和半胱氨酸殘基。
  8. Human gnt - v contains 741 amino acids with six potential sites for n - glycosylation and bears high homology to gnt - v of rat. its gene is located on chromosome 2q21 containing 17 exons. gnt - v protein is encoded by exons 2 - 17 as open reading frame

    人類gnt - v由741個氨基酸組成，有6個潛在的n -糖基化位點，基因定位於染色體2q21 ，含有17個外顯子，其開放閱讀框架由外顯子2 - 17進行編碼。
  9. Pcr product was cloned to the downstream of gst gene according to the right open reading frame ( orf ) in pgex - 6p - l vector, and e. coli bl21 was transformed by the recombinant plasmid for expression

    Pcr產物經純化、酶切后，按正確的閱讀框架定向克隆到表達性載體pgex - 6p - 1中谷胱甘肽轉移酶（ gst ）基因的下游。
  10. Nucleic acid sequences of azoreduclase were searched and blasted in genbank. a pair of primers based on the conserved regions were designed. a specific fragment was amplified by pcr from the plasmid of rhodopsedomonas palustris and sequenced. the sequence contained a complete 471bp orf ( open reading frame )

    脫色實驗證明沼澤紅假單胞菌（ rhodopsedomonaspalustris ）對偶氮染料有較強的降解能力，我們通過genbank搜索，對所獲得的所有偶氮還原酶基因在ncbi進行比對並設計引物，從沼澤紅假單胞菌質粒中擴增獲得了一條含471bp完整開放閱讀框架的序列。
  11. Compared with a reported cmv - cp gene sequence, the homology of nucleotide sequences were 100 %. the sequencing result also demonstrated the recombinant vector pet - 22b - cp has a proper orf encoding 218 amino acids. the recombinant vector was transformed into bl21 ( de3 ) cells. transformants were grown and induced by the addition of isothiopropylgalactoside ( iptg ) to a concentration of imm with continued shaking at 37 ?

    酶切鑒定及序列測定表明，重組表達質粒pet - 22b - cp連接區域符合設計要求，具有正確的開放閱讀框架，插入片段含有218個氨基酸的完整編碼區，其核苷酸序列與報道的cmv - cp基因的同源性為100 。
  12. In this research two full - length cdnas have been cloned by a combination of rt - pcr and 3 " - is1 - race with synthesized degenerate primers from young leaves of vicia faba l., pichia methanolica high - level expression systems of the genes have been constructed, and the milligram expressed protein was purified using probond resin purification system, which may result in further identification of the function of the aba binding protein. the full - length cdna of abp370 fragment is 3449 bp long and has an open reading frame of 2304 bp encoding 768 amino acids with 876 bp long 5 ' - utr, 369 bp long 3 ' - utr and poly ( a ) tail. the full - length cdna of abp640 fragment is 1012 bp long and has an open reading frame of 780 bp encoding 260 amino acids with 88 bp long 5 ' - utr, 144 bp long 3 " - utr and poly ( a ) tail

    3 - 5 - race擴增片段序列分析結果表明， abp370擴增片段的全長cdna為3449核苷酸，其中5非翻譯區為876個核苷酸， 3非翻譯區為369個核苷酸並末端帶poly （ a ）尾巴，從起始密碼子atg至終止密碼子tga ，含有一個編碼768個氨基酸殘基的開放閱讀框架（ 2304bp ） ； abp640擴增片段的全長cdna為1012核苷酸，其中5非翻譯區為88個核苷酸， 3非翻譯區為144個核苷酸並末端帶poly （ a ）尾巴，從起始密碼子atg至終止密碼子taa ，含有一個編碼260個氨基酸殘基的開放閱讀框架（ 780bp ） 。
  13. The cdna is 2 149 bp long with an open reading frame of 1 697 bp, which encodes a polypeptide of 565 amino acids, preprotein of 62. 3 kda ; 5 " untranslated regions contains 59nt, 3 " untranslated regions contains 391nt, a poly ( a ) tail signal and long poly ( a ) tail

    該cdna全長2149bp ，包含一個1697bp的開放閱讀框架，編碼565個氨基酸。在起始密碼子上游有一個由59個堿基組成的5 』非編碼區；在終止密碼子下游有一個由391個堿基組成的3 』非編碼區，包括4個分解信號、 1個加尾信號和1個長度為17個腺苷酸的poly （ a ）尾。
  14. The recombinant plasmid was identit1ed with restriction endonuclease, pcr, then sequenced. the resuit of sequence analysis showed that the vp3 gene is 1605bp and inc1uds a complete open reading frame encoding a protein of 534 amino acids. the hl isolate shares 98. 5 % and 98. 3 % identity with b isolate at nucieotide and amino acid levels respectively

    結果表明：鵝細小病毒h1分離株vp3基因全長1605bp ，編碼534個氨基酸，只有一個完整的開放閱讀框架，與國外已發表的鵝細小病毒b株核苷酸序列同源性為98 . 5 ，氨基酸序列同源性為98 . 3 ，表明這二個毒株親緣關系相近。
  15. The cloned s2 gene from lx4 strain was composed of 2023bp in length encoding for a polypeptide of 625 amino acid

    N基因：全序列為1230hp ，編碼409個氨基酸，包含完整的開放閱讀框架。
  16. In chapter three, we investigated the function of hasnpv open reading frame 122 ( hal22 ), which is also present in the closely related h. zea snpv

    Hasnpv基因組中含有20個獨特開放閱讀框架。第三章對hasnpv中獨特基因ha122進行了深入的分子生物學分析。
  17. After sequencing of 84 cdna clones and removing redundant cdnas, we obtained 36 cold - regulated unique cdna clones. 12 cdna clones were expected to be novel genes, because no sequence homology with any known sequences was found in genbank databases

    全長基因ej175共有603bp ，對其可讀閱讀框架進行分析，從57 - 515位核苷酸的一段序列，包含了起始密碼子和終止密碼子，編碼152個氨基酸的多肽。
  18. Dna sequencing of the appa gene showed an open reading frame of 1299 bp. the deduced appa phytase composed of 432 amino acids ( predicted molecular mass, 47. 06 kd ) also contained the reserved active - site motif rhgxrxp, which is shared by other phytases and acid phosphatases

    測序結果顯示appa基因閱讀框架為1299bp ，編碼432個氨基酸，編碼產物理論分子量為47 . 06kd ，同時它也具有其它植酸酶與酸性磷酸酶的活性保守基序rhgxrxp 。
  19. Phaa, phab and phac were inserted to pbv - 220 with double digest of restriction enduonuclease. the expression vectors of pbv - a, pbv - b and pbv - c were constructed by orientaional cloning. indefication of expression vector with restriction enduonuclease digest showed that phaa phab and phac were in right orfs

    將phaa 、 phab和phac片段雙酶切后，定向克隆至原核表達載體pbv220 ，構建了三個原核表達載體pbv - a 、 pbv - b和pbv - c ；經酶切分析表明，所克隆的三個基因phaa 、 phab和phac置於表達載體的正確閱讀框架下。
  20. By cheeking the transformed bacterial colonies, we had filtrated the masculine clone successfully. the sequencing result showed that the inserting fragment contained intact coding sequences of dh. pban ( pheromone biosynthesis activating neuropeptide ) and three sgnps ( suboesophageal ganglion neuropeptide ) and the leading frame of it was correct

    測序結果表明，重組載體的插入片段中含有dh 、性信息素生物合成激活肽（ pheromonebiosynthesisactivatingneuropeptide ， pban ）及三個食道下神經節肽（ suboesophagealganglionneuropeptide ， sgnp ）的完整的編碼序列，且其閱讀框架（ readingframe ）完全正確。
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