chloroplast gene 中文意思是什麼
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These include phototropism, light - driven chloroplast movements and stomatal movements. the phot i gene of arabidopsis encodes an autophosphosphorylating protein kinase that functions as a photoreceptor for phototropism in response to low - intensity bl. up to now
在高等植物中，藍光調控多個重要生理反應過程，其中包括下胚軸的向光反應、葉綠體在光下的重新分佈和氣孔的開放等。 -
To target this mitochondrial enzyme into chloroplast, the cdna sequence of mnsod was fused to a chloroplast transit peptide from a pea rubisco small subunit gene, whereas expression of the chimeric gene was driven by the camv 35s promoter
Pchlsod質粒含有煙草mnsod基因的cdna序列，與豌豆核糖體小亞基葉綠體引物肽（ tp ）的編碼基因序列構成融合基因，由35s啟動子調控。 npt基因為選擇標記基因， pgv2260為輔助質粒。 -
Three chloroplast transformation vectors including pds16s - cat, ptn1269 - bar and psp72 - n5 - bar - n3 were constructed, using ! 6s rrna or chln gene sequence as a homologous segment and cat or bar as a selective marker gene, respectively. foreign genes were introduced to the cells of d. salina by microprojectile bombardment method and a pilot chloroplast tran
3 .杜氏鹽藻葉綠體165出na基因的克隆和轉化載體的構建根據杜氏鹽藻的近緣藻類的葉綠體基因組序列資料，克隆了杜氏鹽藻葉綠體16srrna基因部分序列1100bp ，並利用克隆的16srrna鄭州大學2003年博士學位論文 -
In the third part of the thesis, a chlamydomonas reinhardtii chloroplast expression vector, pactbvpl, containing the fusion of the foot and mouth disease virus ( fmdv ) vp1 gene and the cholera toxin b subunit ( ctb ) gene was constructed. transformation of c. reinhardtii chloroplast was achieved by biolistic bombardment with pactbvpl
論文第三部分主要敘述了將o型fmdvvp1與強黏膜免疫佐劑霍亂毒素b亞基（ ctb ）的融合基因克隆重組到衣藻葉綠體表達載體中，並採用基因槍法轉化衣藻葉綠體，獲得了具有壯觀黴素抗性的轉化子。 -
The chloroplast shsp gene was screened from the cdna library of tomato flower by pcr strategy and confirmed by sequencing. but difference was found at 3 bases of the sequence from the reported in genbank. then, an integrated vector prok ii of the chloroplast shsp gene and nptii gene ( a kanamycin resistant gene ) with camv35s promoter was constructed and introduced into tomato mediated by agrobacterium tumefaciens lba4404. transgenic tomato were screened by their ability of growing on media containing kanamycin
本實驗採用pcr方法從番茄花cdna文庫中克隆到葉綠體shsp基因，經測序證實與genbank中已發表的序列在編碼區相差2個堿基，其中一個堿基導致1個氨基酸的改變。將葉綠體shsp基因定向克隆于帶有組成性表達啟動子camv35s的植物表達載體prok中，凍融法轉化農桿菌lba4404 ，利用葉圓盤法對番茄進行ti質粒介導的遺傳轉化。 -
Ii. molecular phylogeny in this paper, nucleotide sequences of the chloroplast gene trnl - f region was investigated first in the ferns, the pcr primers were chosen. in the test, 34 species in athyriaceae and 3 outgroup taxa were determined trril - f region sequences
介蔗屬d盡口athyrium 、蛾眉蔗屬lunathyrium 、假蹄蓋蔽屬athyriopsis 、單葉雙蓋蔗diplaziumsubsinuatum組成一支，在兩種系統樹中均得到100 %的支持率。 -
Gene silencing, a common phenomenon in phb production through nuclear transformation, had n ' t been observed in the chloroplast transgenic plants analyzed by northern dot blot
Northern點雜交檢測表明與phb合成相關的三個基因均能在轉錄水平表達，未出現核轉化中經常發生的「基因沉默」現象。 -
Because there are many copies of chloroplast dna and the chloroplast has a strong tolerance to accumulation of the products expressed by the introduced foreign gene, a high level of expression is often happened in chloroplast transformation. in addition, because of prokaryotic property of the chloroplast, the prokaryotic gene can be expressed in chloroplast without any modification and multigene can be simultaneously transferred in " polycistron ", which is impossible in nucleic transformation
另外，由於葉綠體基因組的原核性質，對來自原核生物的外源基因無需改造就可以在葉綠體內高效表達，而且可以將多個外源基因採取「多順反子」的原核表達形式同時引入，並由共同的啟動子控制，既方便操作又可避免由於存在多個相同啟動子所帶來的「共沉默」 。 -
Compared with nucleic genome, the chloroplast genome is very small and easily to be manipulated genetically, and the foreign gene is site - direct integrated into the chloroplast genome through homologous recombination
與核轉化相比，葉綠體基因組小，遺傳操作簡單，外源基因是通過同源重組機制定點整合進葉綠體基因組。 -
The result demonstrated that the ctbvpl fusion gene was inserted into the c. reinhardtii chloroplast genome and after three rounds of spc selection the recombinant chloroplast dnas were the majority, and the wild - type chlorop last dnas
W七sternblot和elisa免疫雜交分析表明ctbvpi融合蛋白在衣藻葉綠體中得到表達，表達量占可溶性總蛋白量的3一4 % （ lmg可溶性總蛋白含有30一40pg的重組蛋白） 。 -
In order to form a chloroplast transformation system of d. salina, we have conducted some studies including its sensitivity to antibiotics, the activity of promoter, cloning of the chloroplast genes and construction of transformation vectors, so far a pilot transformation system of the d. salina chloroplast has been completed. methods : the sensitivity of d. salina to seven antibiotics or herbicide used commonly in gene engineering was studied and the biological activity of atpa promoter from c. reinhardtii chloroplast was tested by using enhanced green fluorescent protein ( egfp ) as a reporter. primers were designed in the conservative encoding regions according to the chloroplast genomes from four algae which have close genetic relationship with d. salina, and the sequences of 16s rrna, chll and chln of d. salina chloroplast were cloned and sequenced, respectively
方法:根據杜氏鹽藻的近緣藻類的葉綠體基因組序列資料，在基因編碼區的高度保守區域設計引物，克隆了杜氏鹽藻葉綠體165識na基因、咖l基因和ch n基因，並分別以165識na基因和chln基因序列為同源片段，以cat和bar基因為篩選標記基因構建了三套杜氏鹽藻葉綠體轉化載體: 2鄭州大學2003年博士學位論文杜氏鹽藻（ d ~ iiellasalina ）葉綠體轉化研究pds165一eaf 、 ptn1269一bar和psp72一5一bar一3 ，用基因槍法轉化杜氏鹽藻，初步建立起杜氏鹽藻葉綠體轉化體系。 -
In the second part of the thesis, we described that a tobacco chloroplast expression vector, ptrvp1, containing the foot and mouth disease virus ( fmdv ) vp1 gene and the selective marker aada gene was constructed and transfered to the tobacci chloroplast genome by the biolistic method
論文第二部分主要敘述了煙草葉綠體表達載體ptrvp1的構建，並通過基因槍方法轉化煙草葉綠體基因組，獲得了3株具有壯觀黴素抗性的轉化再生植株。 -
The genomics dna of the transformants was extracted and assayed by pcr with nptii primer camv35 / cp primer and the results indicated that the chloroplast shsp gene has been integrated into the genomics of the tomato. then the transgenic tomato were exposed to low temperature ( in winter, on natural condition, the top temperature was 15 ? and the lowest temperature was 5 and a set of physiology parameters were measured after 6 weeks. the results were shown as follows : 1 ) effect on growth height of the transgenic tomato and the control plants after 6 weeks at low temperature showed that the transformants had been grown faster than the control. in addition, the leaves of the control plants appeared to be much reder than the transgenic tomato, and the change were obvious followed by far from the treated time at low temperature, which suggested that the constituently expression of the chloroplast shsp had some protective fountions to the tomato at low temperature
提取轉基因番茄基因組dna ，分別以npt和35s cp引物對其進行pcr分析，結果表明葉綠體shsp基因已整合進番茄基因組中；對轉基因番茄進行低溫處理（冬季，自然條件下（無加熱的溫室） ，白天最高溫度15 ，夜間最低溫度5 ） ，生長6周后，檢測轉基因番茄的系列生理指標，主要結果如下： 1 ）生長勢：測量轉基因番茄與對照（未轉基因番茄）的株高，結果顯示轉基因植株生長明顯快于對照，且從外觀上看到對照葉片發紅程度遠大於轉基因植株，隨著低溫時間延長，對比更加明顯，說明葉綠體shsp的組成性表達在低溫下對番茄具有一定的保護作用。 -
Chloroplast genetic engineering, a new technology that could overcome many problems associated with nuclear genetic engineering, is of growing interest for bioproduction of valuable materials. it has several advantages including the extraordinarily high level of transgene expression and environmental safety, the favorable environment for prokaryote gene expression, the absence of " position effect " and gene silencing
葉綠體基因工程是隨著植物遺傳轉化技術發展剛剛興起的生物技術，具有超量表達外源基因，為原核基因提供適宜表達環境，消除「位置效應」和基因沉默，環境安全性好等優點，較更適合用於植物生物反應器方面的研究。 -
These include the ability to target the gene of interest to site - specific areas of the chloroplast genome, high - level expression of foreign genes and increased environment security
由於植物葉綠體表達系統能夠高效表達目的基因、環境安全性高，已成為目前植物生物反應器研究的熱點之一。 -
To investigate whether it is suitable for biodegradable plastic production, we constructed the chloroplast integration and expression vector ptrv - phb, and integrated all the genes necessary for phb synthesis into tobacco chloroplast genome through gene - gun transformation. the chloroplast transformants showed phenotypically normal growth and were fertile
本研究在國內率先探討將葉綠體轉化技術引入植物生產生物可降解塑料這一領域的可行性（國外僅有日本一例） ，構建了葉綠體轉化及表達載體ptrv - phb ，通過基因槍法將phb合成相關基因導入煙草葉綠體基因組。 -
To determine the site - specific integration of the foreign gene into the chloroplast genome and the level of homogeneity, the three transformation lines of the second selective generation were analysized through pcr ( pcr - southern blot ) analysis using the primers of fmdv vp1 gene and homologous fragment of tobacco chloroplast, respectively
經過兩輪抗生素篩選后，採用fmdvvp1基因的一對引物和煙草葉綠體同源片段的一對引物分別對抗性植株的葉綠體dna進行的pcr和pcr - southernblot分析。 -
Western blot and elisa assays indicated that fmdv vp1 gene was expressed in tobacco chloroplasts and accounted for 2 % ~ 3 % of the total soluble protein ( about 20 - 30 ( ig of chloroplast - expressed vp1 protein in 1 mg of total soluble protein )
Westernblot和elisa免疫雜交分析證明fmdvvp1抗原蛋白在煙草葉綠體中得到表達，表達量占可溶性總蛋白量的2 3 （ 1mg可溶性總蛋白含有20 30 g的vp1蛋白） 。
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