比克隆 的英文怎麼說
中文拼音 [bǐkèlōng]
比克隆
英文
birkelund-
Thomas j. abercrombie, kabul, afghanistan 1967, as enclosed as her pets, an afghan woman secludes herself behind the traditional chadri as she balances caged goldfinches bought at market
阿富汗，喀布爾， 1967年，托馬斯?亞柏克隆比，這位婦人將市場買來的金翅雀頂在頭上，一襲傳統的察都里袍圍住全身，把她圍得與籠中鳥一樣的禁閉。Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss
將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。Compared with the known antibodies, the antiserum we prepared is highly specific and rules out the possibility of cross - reacting with angiotensin fragments. 4
與己有抗體相比，本實驗制備的抗agt多克隆抗血清不僅具有較高的特異性，而且排除了與血管緊張素短片段的交叉反應。Considering of the specificity of the degenerative primer designed in this pcr reaction, the identity between the sequence we wanted and the fragment of pcr product and the presence of asmaspa, asmaspb, clr and cls ( the homologous gene of masp gene ) in halocynthia roretzi, a japanese ascidian, we believe that the sequence of pcr product is some part of the masp gene or masp homologous gene
基於本實驗中所設計的引物為特異性簡並引物，測序基因通過比較得到與預期片段有一定的同源性以及masp同源物asmaspa 、 asmaspb 、 clr和cls在海鞘中存在的事實，我們可以初步推測，本實驗pcr反應所克隆的片段可能為文昌魚masp或其同源基因的一部分序列。Opium, morphine, heroin, methadone, narcotic analgesics, cannabis, lsd, cocaine, ketamine, gamma hydroxybutyric acid ( ghb ), amphetamine ( e. g. methylamphetamine, ecstasy, phentermine ( duromine ) ), benzodiazepine ( e. g. chlordiazepoxide ( librium ), midazolam ( dormicum ), estazolam, flunitrazepam ( rohypnol ), triazolam, nimetazepam, diazepam ( valium ) ), zopiclone, barbiturate ( e. g. quinalbarbitone ), methaqualone ( mandrax ), hallucinogen, depressant, stimulant or tranquillizer
（俗稱綠豆仔） 、咪達唑侖（俗稱藍精靈） 、舒樂安定、氟硝西泮（俗稱十字架） 、三唑侖、硝甲西泮、安定（俗稱羅氏五號、羅氏十號） ） 、佐匹克隆、巴比士酸鹽（如速可巴比妥、甲?酮（俗稱忽得） ）及其他迷幻劑、鎮抑劑、興奮劑及鎮靜劑。The sobriquet of la carconte had been bestowed on madeleine radelle from the fact that she had been born in a village, so called, situated between salon and lambesc ; and as a custom existed among the inhabitants of that part of france where caderousse lived of styling every person by some particular and distinctive appellation, her husband had bestowed on her the name of la carconte in place of her sweet and euphonious name of madeleine, which, in all probability, his rude gutteral language would not have enabled him to pronounce
卡爾貢特娘們這個綽號的由來，是因為她出生的村莊位於薩隆和蘭比克之間，那個村莊就叫這個名字。而據卡德魯斯所住的法國那一帶地方的風俗，人們常常給每一個人一個獨特而鮮明的稱呼，她的丈夫之所以稱她卡爾貢特娘們，或許是因為瑪德蘭這三個字太溫柔，太優雅了，他那粗笨的舌頭說不慣。In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。In the experiment, in case of the proliferative capability of eg cells, the composition of 0. 25 % trypsin + 0. 04edta was optimal. the growth behavior of icr murine eg cells from pgc was observed
傳代過程中，以0 . 25胰酶與0 . 04 edta聯合作用效果較好，對eg細胞的綜合損傷最小，離散傳代后eg細胞活力比較強， eg克隆出現最多，最高傳至6代。At first. then eight a - amylase gene fragments were cloned with the genomic dnas as templates by routine pcr. following that, these gene fragments and plasmid vectors, pbluescript ii ks + and puc18, were cut by bamh i and kpn i. the prepared insert dna and vector dna were linked by t4 dna ligase
利用vectornti6 . 0軟體，對所克隆的序列用相鄰接點法（ neighborjoining州j ） method ）進行多序列比對，分析其同源性，並構建基因進化樹。Sp2 / 0 myeloma cells are fused with spleen cells of balb / c mice ( the ratio is 1 : 10 ) immunized with at - by routine method. positive clones are detected by elisa
Sp2 / 0細胞和免疫脾細胞按1 ： 10比例融合，融合劑peg分子量為1500 ，陽性克隆篩選採用三步進行。These observations show that reprogramming is easier in interspecific embryos reconstructed with es cells than that reconstructed with somatic cells, and that es cells have the higher ability to direct the reconstructed embryo development normally than fibroblast cells. oocytes were reconstructed with outbreeding kunming albino mouse es cells and enucleated rabbit oocytes, and the effects of the passages of es cells and 6 - dmap on the development of interspecific reconstructed oocytes were analyzed. the interspecific reconstructed es - rabbit oocytes were activated either by combined two set electric pulses and 6 - dmap or by two set electric pulses
以上結果顯示， 6 dmap能增加胚胎幹細胞異種重構卵的卵裂率，對重構卵囊胚的發育率影響不大；高培養代數和低培養代數的胚胎幹細胞用於異種核移植不影響異種重構卵的卵裂率和囊胚發育率；用胚胎幹細胞作為供核細胞；比體細胞作為供核細胞所構建的異種重構胚更容易進行重編程，並且胚胎于細胞指導異種克隆胚胎正常發育的能力強于體細胞。Molecular cloning of 5 ' flanking region of ovine keratin associated protein 6 - 1 gene and comparison of the sequences
端調控區的分子克隆及測序結果比較The result showed that the homology rate of pila gene among the 5 avian pathogenic e. coli strains tested and one human e. coli were from 89. 8 % to 91. 1 %, and the homology rate of amino acid were from 88. 5 % to 91. 8 %. the homology rate of pila gene sequence among 5 avian pathogenic e. coli strains tested and avian pathogenic e. coli reported ( serotype o1, o2, o78 ) were from 87. 8 % to 90. 2 %, and the homology rate of amino acid were from 84. 6 % to 91. 2 %. there had homology in avian pathogenic e. coli. there had some common antigen side in type 1 pili of avian pathogenic e. coli
結果表明：運用msha法檢測1型菌毛的檢出率為80 （ 36 45 ） ， pcr法的檢出率為95 . 5 （ 43 45 ） ， pcr方法用於1型菌毛的檢測比msha更加敏感、快速、特異性強；選擇5株優勢血清型雞源致病性大腸桿菌代表株（ o _ （ 89 ） ， o _ （ 119 ） ， o _ （ 141 ） ， o _ （ 127 ） ）的1型菌毛pila基因的pcr擴增片段經純化后，分別定向克隆到puc18質粒的多克隆位點，構建了含有目的基因片段的克隆質粒，並轉化到dh5株大腸桿菌載體菌中，篩選獲得陽性克隆菌株。Cost - compared to brand - name computers the clone will generally be easier on your pocket book when purchasing one with similar features as its brand - name counterpart
成本比品牌電腦克隆一般容易對您當初購買一個類似袖珍手冊其特點為品牌對應With extension of the stolon, changes of the internode length, branching intensity and ramet length were fit to exponential model. it seemed to " flexibly " adapting to the habitat because the clonal architecture of s. vulgaris had both the traits of " guerilla " and " phalanx "
分株長度、間隔物長度和分枝強度隨匍匐莖延伸呈指數模型變化，其克隆構型兼有「游擊」型和「密集」型的特點，能比較「靈活」的適應環境。Sequence alignment shows that the fragment of pcr product showed some identities to the masp gene of xenopus laevis, branchiostoma belcheri, the trypsin gene in litopenaeus vannamei and the serine protease gene in aurelia aurita
Pcr反應產物基因片段大小為630bp ，序列比較結果表明， pcr克隆產物與爪蟾、文昌魚的masp基因，蝦、水母等的胰蛋白酶基因和絲氨酸蛋白酶基因都有一定的同源性。Meanwhile, these six coat protein genes were sequenced and compared with other homologous sequences in genbank. the strain designation of the six isolates of tumv was finally determined. the results are as the following : 1. six isolates of turnip mosaic virus named tumv - sd1, tumv - sd2, tumv - sd3, tumv - sd4, tumv - sd5 and tumv - sd6 were respectively acquired from infected chinese cabbages and radishes in 3 cities ( taian, yantai and zaozhuang ) of shandong province
利用rt - pcr方法克隆了tumv山東分離物的外殼蛋白（ cp ）基因，測定了它們的核苷酸序列、並將其與已報道的序列進行同源性比較和分析，最終確定了其歸屬地位，具體研究結果如下： 1 .從山東省3地市感病的白菜和蘿卜上分離到蕪菁花葉病毒的6個分離物，分別命名為tumv - sd1 、 tumv - sd2 、 tumv - sd3 、 tumv - sd4 、 tumv - sd5和tumv - sd6 。By blasting the homologous sequences in genbank databases, the sequence of grass carp gh cdna from pituitary is 98 % homologous compared with the previously cloned gh cdna of grass carp. the cgh cdna fragment was inserted into pgex - 4t - l to construct the expression plasmid. the recombinant plasmid was digested by bamh i and ecor i to identify whether the cgh cdna fragment was inserted into the plasmid, the pgcgh was transformed into e. coli bl21 competent cells
將得到的序列在genbank和embl數據庫中進行了同源比較，結果顯示：本研究克隆到的草魚gh基因與genbank中登記的x60474草魚gh基因有12個堿基的差異，編碼的氨基酸有3個氨基酸殘基的差異，同源性為98 ，影響蛋白質高級結構的保守二硫鍵為2個。Clonal plants present in different ecosystems and take important role in most of the ecosystems
同非克隆植物相比，克隆植物大多具有更強的適應環境壓力的能力。分享友人