活體鑒定 的英文怎麼說

中文拼音 [huótǐjiàndìng]
活體鑒定 英文
bioassay
  • 活 : Ⅰ動詞1 （生存; 有生命） live 2 [書面語]（救活） save （the life of a person）:活人無算 （of a goo...
  • 體 : 體構詞成分。
  • 鑒 : Ⅰ名詞1 （鏡子 古代用銅製成） ancient bronze mirror2 （可以作為警戒或引為教訓的事） warning; objec...
  • 定 : Ⅰ形容詞1 （平靜; 穩定） calm; stable 2 （已經確定的; 不改變的） fixed; settled; established Ⅱ動詞...
  • 活體 : living body活體檢查 biopsy; 活體鑒定 [法律] identification of the living
  • 鑒定 : 1 （評語） appraisal （of a person s strong and weak points） 2 （評定） appraise; identify; auth...
  1. In the experiment, the full code sequence of bar gene was cloned by pcr from transgenic herbicide resistant bobwhite wheat and checked. it was expressed in e. coli and its protein was determined. after having been properly modified, the bar gene which correctly codes pat was cloned into binary vector pbi121 and then transferred into lba4404 by triparantal crossing, which is the prerequisite work for genetic transformation

    本實驗從抗除草劑轉基因bobwhite小麥中，利用pcr克隆的方法擴增出bar基因全長，並在原核表達系統中表達，鑒定表達蛋白的活性，將能夠正確編碼ppt乙酰轉移酶的bar基因片段，經過適當的修飾構建入真核表達載體。
  2. A bacteria strain, numbered dbd11, was isolated from the oil and water mixture sample gathered from the changqing oil field. it is able to make crude oil as the sole silicon source and generate biosurfactant, organic acid and biogas

    從長慶油田油水樣中分離出一株能以原油為唯一碳源產生生物表面活性劑、有機酸、氣體的菌株（編號為dbd _ （ 11 ） ） ，經過鑒定為嗜麥芽窄食假單胞菌。
  3. This part firstly states some of the main problems, and then puts forward the constructive systems including the system of open of authentication act, discovery of expertise prior to court, the system of stating, questioning and cross - examine, and the system of recognition for expertise. all these systems tend to be practical and continuous ; and in each of the system also include some concrete theoretical and practical elements

    本部分首先對這些問題作了一個大致的歸納性闡述;而後針對這些具體問題，更從一個系統解決問題的角度出發，提出了由鑒定活動公開制度、鑒定結論庭前展示制度、鑒定結論質證制度以及鑒定結論認證制度四部分組成的鑒定結論審查評斷的程序設計。
  4. Techniques of culture of prothoracic gland ( pg ) in vitro, 3h - ecdysteroid - radiounniunoassay, and methods of molecular biology and biophysics, for instance, nuclear magnetic resonance ( nmr ), circular dichroism cd ) and so on were engaged. the biological activity on ecdysteroidogenesis in vitro and in vivo of a group of analog peptides of bom - ptsp synthesized by chemical modifications and the relationship between the structures and functions were studied

    本研究利用~ 3h -蛻皮激素放射免疫分析方法、分子生物學、生物物理學的方法和手段、體外培養和體內導入等技術對bom - ptsp及其一組人工合成的與ptsp結構類似的多肽進行了結構和功能關系的研究，鑒定了它們的生理活性及其與結構的關系，以及它們在家蠶體內的活性及其與結構的關系，還對ptsp及其類似多肽進行了核磁共振和圓二色的研究。
  5. The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins

    將帶有hwtx -基因的ppic9k經blgii線性化后，轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌，進一步用遺傳毒素g418篩選多拷貝的轉化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導表達，發酵上清經90飽和度的（ nh _ 4 ） _ 2so _ 4沉澱， yw - 3 （ mwc03000 ）的超濾膜超濾，再經cm陽離子交換， c _ （ 18 ）反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經質譜鑒定，氨基酸組成分析和序列測定為正確的表達產物，生物學活性表明其活性為天然毒素活性70 % ，表達量為80mg / l 。
  6. To the infiltration of social life of various fields with science and technology, modern dispute and science have been already inseparable, the scientific evidence becomes the new " king of the evidence ", has formed the enormous impact to judge ' s judgement right. this text set forth one ' s views in the platform with the judge how to examine and assert scientific evidence, how to restrain invasion and attack to the mechanism of dispute solves from expansion of expert discourse - right

    關于科學證據的文章可謂汗牛充棟，其中絕大多數是對所謂鑒定體制的批評與設計，對于科學證據的基本問題以及法官如何審查認定科學證據的論文卻寥若星辰。隨著科學技術向社會生活各個方面的滲透，現代糾紛與科學已經密不可分，科學證據成為新的「證據之王」 ，對法官裁判權形成了巨大的沖擊。
  7. Course contents ( containing practice period ) : introduction to the course ; basic function of cells ; breath ; blood ; blood circulation ; digesting and absorption ; energy metabolism and body temperature ; the excretion of kidney ; sensory organs ; nervous system ; endocrine system ; urogenital system ; physical constitution ; change of physiological function of human body in each sports stage ; lung ' s function measurement ; blood type test ; hemoglobin measurement ; auscultation of heart sounds ; measurement of blood pressure ; electrocardiogram measurement ; measurement of visual field ; measurement of body temperature ; cardiovascular function test before and after sport

    課程內容（含實踐活動） ：緒論、細胞的基本功能、呼吸、血液、血液循環、消化與吸收、能量代謝和體溫、腎臟的排泄、感覺器官、神經系統、內分泌、生殖、身體素質、運動各階段人體生理功能的變化、肺功能測定、血型鑒定、血紅蛋白測定、心音聽診、血壓測定、心電圖測定、視野測定、體溫測定、運動前後心血管功能變化。
  8. Industrial projects refers to the major projects and accessory facilities completed which result in forming production capacity and have been checked and accepted while the living and welfare facilities have been completed and can ensure normal production and formally put into production

    工業項目是指設計文件規定形成生產能力的主體工程及其相應配套的輔助設施全部建成，經負荷試運轉，證明具備生產設計規定合格產品的條件，並經過驗收鑒定合格或達到竣工驗收標準，與生產性工程配套的生活福利設施可以滿足近期正常生產的需要，正式移交生產的建設項目。
  9. The gpa1 gene was obtained via pcr amplification and was cloned in the two - hybrid dna binding domain vector pgbkt7 the combinant plasmid was designated as pgbkt7 - ga. - galactosidase assay indicated that got did not have the property of self - activation. after pgbkt7 - ga was transformed to yeast pj69 - 4a, we transformed arabidopsis vegetative tissue two - hybrid cdna library plasmids to yeast pj69 - 4a containing pgbkt7 - ga

    通過pcr擴增得到gpa1基因並將其克隆到雙雜交dna結合域載體pgbkt7中，得到的重組質粒命名為pgbkt7 - g ， ?半乳糖苷酶活性鑒定表明g不具有自激活特性，將其轉化到酵母菌pj69 - 4a中，再以此為受體菌轉化擬南芥綠色營養組織cdna文庫質粒。
  10. Meanwhile the depiction of mycelia could contribute the identification among species. eight extracellular anzymes of mycelia from phellinus igniarius cultured by shaking were studied for the first time

    同時通過對不同生長時期菌絲體形態的觀察描繪，為桑黃的品種鑒定提供依據，可避免因品種混雜而影響桑黃的生物活性。
  11. Our school held various training in our school such as cbt delivery, assessment and evaluation, work task research, mis, and so on, more than 130 person time in all

    積極開展形式靈活、內容多樣的學校內部培訓。主要培訓內容包括能力本位教學方法、教學鑒定與評估、工作任務調研分析方法、 mis系統軟體操作培訓等，達130多培訓人次。
  12. Analysis and countermeasures on the difference experts reports of injury assesment between clinic and forensic medicine

    活體損傷醫院診斷與法醫鑒定結論差異原因分析及對策
  13. Physiological function, functional mechanisms of bifidobacterium and its applications in foods were investigated systematically by seven experiments in vitro and in vivo. they were composed of isolation and determination of bifidobacterium, comparison of growth characteristics, factors influencing survivability, bioantaganism to pathogen, adherence and colonization to mucus membrane, immunomodulation mechanisms on trial animals and development of products containing bifidobacterium

    所以本課題通過體內及體外實驗方法，共計七個實驗，包括所研究菌的分離與鑒定；生長特性的研究；影響其存活因素的研究；對病原菌的生物拮抗：對腸粘膜的粘附與定植；對試驗動物的免疫調解機理以及含雙歧桿菌產品的開發，系統研究了該菌的生理功能與作用機理及其應用。
  14. The 102 strains which can produce hydrolyzed circles were obtained using alternative medium containing phytate - calcium. after being isolated and purified, these strains were inoculated into fermented medium, shaking in 28 c at 220r / min for 5 days, then their enzymatic activities were determined by ammonium molybdate - phosphate colorimertry under the condition of 37 cand ph2. 5. the result showed there were 24 strains with higher enzymatic activities among the 102 strains, after the rescreening, 7 strains were gained with enzymatic activities beyond 15u / ml and stable ability of producing acidic phytase, of which, enzymatic activity of the strain 14 was the highest, reaching 53. 86u / ml, and it was preliminarilly identified as aspergillus. niger, then numbered as aspergillus. niger 14

    用植酸鈣選擇性平板培養基從土樣中篩選出了102株能產透明圈的菌株，經分離純化后，接入液體發酵培養基， 28 、 220r min發酵5天，在37 、 ph2 . 5條件下用釩鉬酸銨法測定其所產植酸酶的活力，結果顯示，酶活較高的有24株，經再次搖瓶復篩后，酶活大於15u ml且產酶性能穩定的共有7株，其中以14 ~ #菌株的酶活最高，可達53 . 86u ml ，經初步鑒定為黑麴黴，編號為aspergillus . niger14 ~ # 。
  15. Western blot analysis showed that rhpk - 5 ( recombinant human plasminogen kringle 5, rhpk - 5 ) protein was recognized by mab same as native hpk - 5. the result suggested that we obtained correct gene sequence of hpk - 5 and got the purified rhpk - 5. section ii : construction of pbv220 / hpk - 5 vector for obtaining high - level hpk - 5 expression system, the hpk - 5 gene was recombined with plasmid pbv220 to construct the vector of pbv / hpk - 5

    Coli ）作為宿主，經sds - page分析，篩選表達量最高的菌株作為發酵用工程菌株；用western - blot方法鑒定hpk - 5因子的免疫學活性；用搖瓶發酵的方法，研究發酵培養基的體積（溶解氧） 、組成成份及誘導起始時間和誘導持續時間對目的蛋白表達量的影響，優化hpk - 5基因工程菌的表達條件。
  16. The biosurfactant tested preliminarily is lipopeptide or lipoprotein and the average content in fermentation solution per 100ml is 1. 4g. the organic acid is formic acid and acetic acid and the average content in fermentation solution per 100ml is 202mg. the average biogas output is 12ml from fermentation solution per 100ml

    產生的生物表面活性劑初步鑒定為脂肽或脂蛋白，平均每100ml發酵液可得干物質1 . 4g ；產生的有機酸為甲酸、乙酸混合物，平均每100ml發酵液含量為202mg ；平均每100ml發酵液產生氣體12ml 。
  17. In addition, whether and how the bl receptor photl regulate ca2 + - channels in the pm has not been investigated. in present study we aimed to identify ca2 + channels involved in bl signaling cascade. the patch clamp technique was applied to record the channels on the hypocotyl protoplasma of wild type and photoreceptor mutant of arabidopsis thaliana

    採用膜片鉗技術在擬南芥下胚軸原生質體質膜上記錄並鑒定到一種超極化激活的鈣離子通透性通道，對其特性分析發現photl - 5突變體與野生型擬南芥的質膜鈣離子通透性通道電流有顯著差異，該差異可能是二者對胞外鈣表現不同反應的原因。
  18. Objective : to construct the single - chain fv gene of human anti - hbsag and to analyse the expression of the constructed gene in eukaryotic cell and prokaryotic cell

    目的構建人抗hbsag單鏈抗體基因，在真核細胞和原核細胞中進行表達，並對其表達產物的活性進行鑒定。
  19. The positive transformants with the integrates mn - sod gene and cuzn - sod gene were identified by zeocin - resistance, pcr screening and expression in p. pastoris. the recombinant mn - sod protein and cuzn - sod protein was successfully expressed in pichia pastoris based on the evidences that the obvious activity of sod existed in native - page and enzymatic activity test

    Pcr鑒定進一步說明，目標基因已經重組到宿主基因組染色體上； 0 . 5甲醇誘導表達后，活性電泳出現明顯活性條帶，重組酵母發酵液中mn - sod的活性約是對照菌株sod活性的2 . 6倍； cuzn - sod酶活力約是對照菌株sod活性的1 . 3倍。
  20. The positive transformants with the integrates mn - sod gene was identified by zeocin - resistance, pcr screening and expression in p. pastoris. the recombinant mn - sod protein was successfully expressed in pichia pastoris based on the evidences that a relative molecular weight about 23kd appeard in sds - page, the obvious activity of sod existed in native - page and enzymatic activity test, and mn - sod activity was specific base on the inhibition with the mixture of chloroform - enthanol ( 3 : 5 / v : v ) and potassium cyanide. two secreted plasmids ppiczaa - sodm18 and ppiczaa - sodc were constructed and after there linearization were transferred into chromosome of pichia pastoris gs115 by electroporation

    Pcr鑒定及mut表型分析進一步說明，目標基因已經重組到宿主菌基因組染色體上； 0 . 5甲醇誘導表達后， sds - page結果顯示，表達的蛋白相對分子量約為23kd ，活性電泳出現明顯活性條帶；酶活性測定顯示，重組菌株sod活性比對照提高5倍左右；氯仿-乙醇（ 3 : 5 v : v ）和kcn （ 5mmol l ）抑制反應進一步證明，所表達的sod為錳超氧化物歧化酶。
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