膜分子生物學 的英文怎麼說
中文拼音 [mófēnzishēngwùxué]
膜分子生物學
英文
molecular biology of membrances- 膜 : 名詞1. [生物學] (像薄皮的組織) membrane 2. (像膜的薄皮) film; thin coating
- 分 : 分Ⅰ名詞1. (成分) component 2. (職責和權利的限度) what is within one's duty or rights Ⅱ同 「份」Ⅲ動詞[書面語] (料想) judge
- 子 : 子Ⅰ名詞1 (兒子) son 2 (人的通稱) person 3 (古代特指有學問的男人) ancient title of respect f...
- 生 : Ⅰ動詞1 (生育; 生殖) give birth to; bear 2 (出生) be born 3 (生長) grow 4 (生存; 活) live;...
- 物 : 名詞1 (東西) thing; matter; object 2 (指自己以外的人或與己相對的環境) other people; the outsi...
- 學 : Ⅰ動詞1 (學習) study; learn 2 (模仿) imitate; mimic Ⅱ名詞1 (學問) learning; knowledge 2 (學...
- 生物學 : biology
- 生物 : living things; living beings; organisms; bios (pl bioi bioses); biont; thing; life生物材料 biol...
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Abstract : in this paper interactions of bilayer lipid membrane with clotrimazole as the antifungal agent were reported in order to interpret the action basis about the molecularbiology of this drug. using the injecting method , we prepared three bilayer lipid membranes ( blm ) respectively, and by the cyclic voltammetry method we determined properties of blm before and after adding clotrimazole into the membranes. it was found that clotrimazole could remarkably reduce the membrane resistance and demonstrated that the drug as an antifungal substance might certainly change the permeability of bilayer lipid membrane
文摘:報道了抗真菌藥克霉唑與人工脂膜的相互作用,試圖闡明該藥的分子生物學的作用基礎.用注射方法分別制備3種雙分子層脂膜( blm ) ,並運用循環伏安法測量了修飾物克霉唑加入前後人工雙分子層脂膜的電性質.結果發現克霉唑可顯著降低膜電阻並證實該藥確能改變人工雙分子層脂膜的通透性Such examples are given as the blood filtering principal of hemoglobin, catalysis of enzymes, immune recoglization, prion, glycoprotein and the relationship of structure and function of membrane protein, et al, as well as the applications to medicine
在簡要介紹結構生物學的研究方法的基礎上,主要從分子水平闡述蛋白質和核酸的結構原理、相互作用、結構與功能的關系,通過具體實例闡述血紅蛋白的輸氧機制、酶的催化機制、免疫分子識別、朊病毒、糖蛋白、生物膜的結構功能關系等,以及結構生物學在醫學上的應用。It builds a certain methods on biological study of e2 gene by this expriment through designing primer meticulously and cloning of envelope glycoprotein gene e2
本實驗通過引物設計及bvdv囊膜糖蛋白e _ 2基因的克隆,為e _ 2基因的進一步分子生物學分析及亞克隆奠定了的基礎。Using water - soluble carbodiimide as condensation agent, biomacromolecules such as gelatin, collagen and chitosan were covalently grafted on the plla - g - pmaa membranes. solution of the biomacromolecules was further coated on the above membranes to increase the surface density of the biomacromolecules. this technology was designated " grafting and coating " method
為了將生物大分子化學接枝于plla膜表面,首先在plla膜表面接枝pmaa ,將羧基引入plla表面,利用水溶性碳化二亞胺edac作為縮合劑,與含有氨基的生物大分子反應,可將生物大分子如明膠、膠原和殼聚糖化學接枝在plla膜表面。Subject covers all major areas of cellular and molecular neurobiology including excitable cells and membranes, ion channels and receptors, synaptic transmission, cell type determination, axon guidance and targeting, neuronal cell biology, synapse formation and plasticity
本學科涵蓋了細胞分子神經生物學的所有主要領域,包括興奮細胞和細胞膜、離子通道和受體、突觸傳遞、細胞類型決定、軸突導向和定位、神經元細胞生物學、突觸形成和可塑性。In this research, the wheat, rice and tobacco with salt treatment were used as experimental materials to study the molecular mechanism of stress - tolerance in plant and make the relative gene engineering research. the main results are as following : 1. a novel ca2 + channel gene, named as tatpc1, was isolated from wheat, encoding a two - pore voltage - gated channel protein
本研究在總結當前植物鹽脅迫應答分子生物學研究進展的基礎上,以農作物小麥、水稻、煙草作為主要實驗材料,開展了植物耐逆分子機制和相關基因工程研究,主要結果為: 1從小麥中克隆到一個新的ca ~ ( 2 + )通道蛋白基因, tatpc1 ,它編碼的是一種細胞膜上兩孔電壓門控式通道蛋白。The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins
將帶有hwtx -基因的ppic9k經blgii線性化后,轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌,進一步用遺傳毒素g418篩選多拷貝的轉化菌株,命名為gh1 ;將gh1甲醇酵母菌用0 . 5的甲醇誘導表達,發酵上清經90飽和度的( nh _ 4 ) _ 2so _ 4沉澱, yw - 3 ( mwc03000 )的超濾膜超濾,再經cm陽離子交換, c _ ( 18 )反相hplc純化得到分子量為4kd左右的組分,其中4289 . 05的組分經質譜鑒定,氨基酸組成分析和序列測定為正確的表達產物,生物學活性表明其活性為天然毒素活性70 % ,表達量為80mg / l 。Research progress on molecular biology of pathogenic factor of clostridium perfringens type c
型產氣莢膜梭菌致病因子分子生物學研究進展“ mutations in notch 1 cause an early developmental defect in the aortic valve, ” said dr vidu garg, an assistant professor of paediatrics and molecular biology
Notch 1基因突變,引起主動脈瓣膜的早期發育缺陷,小兒科和分子生物學助理教授維杜加爾格博士表示。Progress in research of glycoprotein molecular biology of paramyxoviruses
副粘病毒包膜糖蛋白分子生物學研究進展The research team of prof chan hsiao chang, director of the epithelial cell biology research centre, in collaboration with zhejiang academy of medical sciences, demonstrated that cystic fibrosis transmembrane conductance regulator cftr is involved in transporting bicarbonate into sperm, and thus, is vital to sperm fertilizing capacity and male fertility. cftr is an anion channel, mutations of which cause cystic fibrosis, a disease characterized by defective cl - and hco3 - transport with clinical manifestations in a number of organ systems
由陳小章教授領導的香港中文大學上皮細胞生物學研究中心的研究人員,與浙江醫學科學院合作,證實囊性纖維化跨膜電導調節器( cftr )負責輸送碳酸氫根進入精子,對精子授精能力及男性生育能力非常重要; cftr是一個陰離子通道,其基因突變會導致囊性纖維化,因為氯離子和碳酸氫根( hco3 - )分泌缺陷,引發一系列器官病徵。Cell adhesion to surface of the substrate is essential to development of the anchorage - dependent cells. only after adhering to surface followed by spreading can cells develop and proliferate. most synthetic polymers used as orthopaedic matrix substitute present hydrophobicity, which may correlates to the low degree of cell attachment. modification with cell adhesion protein / peptides can be benificial to the cell adhesion on polymers and then affect the cell proliferation and differentiation. cell attachment to substrate is primarily mediated by integrins, a widely expressed family of heterodimeric surface receptors. most extrcellular matrix proteins such as fibronectin, osteopontin, collagen type i, bone sialoprotein and vitronectin contain an arg - gly - asp ( rgd ) sequence which is specific to the fixation of cell membrane receptors like integrin. the main aim of this research is to measure, assess adhesion, proliferation of rabbit marrow stromal cells ( mscs ) on the polymers coated by fibronectin, collagen type i or biotie gen, which includes : ( 1 ) biologic characteristics of rabbit mscs were observed by two types of separating method in primary culture. ( 2 ) adhesion, proliferation and differentiation of mscs cultured on polymers coated with biotiegen were assessed. ( 3 ) also, adhesion, proliferation and differentiation of mscs were assessed on plga film or porous plga substrates coated with fibronectin, or collagen type i respectively. ( 4 ) bone formation was observed on the porous plga substrates coated with collagen type i in vivo. this research aims to give new way to make novel synthetic bone with cell adhesion and high bone induction capabilities
因此將這些蛋白包被、固定到材料表面,觀察骨組織工程種子細胞mscs細胞的粘附、生長特性是本研究的中心環節,並從以下方面進行探討: ( 1 )採用不同原代細胞分離方法,研究其對mscs細胞的生物學特性影響。 ( 2 )檢測基因勝肽膠對mscs細胞粘附、增殖及分化的影響。 ( 3 )分別採用型膠原及纖維粘連蛋白( fibronectin , fn )包被聚乙醇酸-乳酸共聚物( poly ( 1actide - co - glycolide ) , plga )膜及多孔塊型plga材料,觀察細胞在單層或三維培養狀態下,型膠原及fn對mscs細胞粘附、增殖及向成骨細胞分化效應及能力。The study on the function and mechanism of phrip1 is important for clarifying how the cell plate and cell wall form in plants. in this study, full length of phrip1 is amplified by pcr and ligated into pks plasmid, then the bait plasmid, peg202 - phrip1, is constructed. the inseret gene are sure to be translated into the right fusion protein through its sequence. in the yeast two - hybrid system, the bait plasmid ( peg202 - phrip1 ) and a reporter plasmid ( psh18 - 34 ) are introduced into the yeast ( egy48 ) by co - transformation. then cdna library ( which is in pjg4 - 5 ) is screened and two genes are obtained. the two insert gene fragments are sequenced. one of them is plastocyanin, the other is putative photosystem i reaction center subunit ii precursor, both of them are the necessary components of photosynthetic chain
成膜素相關蛋白1 ( phrip1 )是一個含608個氨基酸的蛋白質,它對于植物胞質分裂中細胞板的形成起到了十分重要的作用。研究phrip1的功能和機制,對在分子水平上闡明植物細胞板以及細胞壁形成的機理具有重大的生物學意義。在本實驗中,根據phrip1的序列設計引物對其進行pcr擴增,得到該基因后將其連接到了pks質粒上,並進一步構建成了誘餌質粒peg202 - phrip1 。Objective : to detect the telomerase activity in human esophageal carcinoma and tumor ? adjacent tissues and investigate the possible molecular mechanism of esophageal carcinogenesis
目的:檢測食管癌及周圍非癌食管粘膜組織中端粒酶活性,了解食管癌發生可能的分子生物學基礎。Recently, a noval brain - specific na ^ - dependent inorganic phosphate transporters was successfully cloned using the method of molecular biology and was named dnpi because they had the characters of the vesicular glutamate transporter ( vglut ). some studies showed that dnpi were distributed extensively in the brain area where glu was used as the neurotransmitter, such as thalamus, medulla oblongata and spinal cord. the ultrastructural studies indicated that most of dnpi are located in the presynaptic terminals and distributed on the synaptic vesicular membrane
最近人們應用分子生物學技術又成功地在哺乳類動物腦內克隆出一種新的腦內特殊的na 」依賴的無機磷酸轉運體( brain七pecificna dependentinorgarucphosphaternsporter , bnpi ) ,並發現它也具有囊泡膜谷氨酸轉運體( vglllt )的特點,因而被命名為dnpi (又稱作vgiutz人新近的研究報道, dn 』 pi廣泛地分佈於運用m作為神經遞質的腦區,如丘腦、延髓和脊髓。The efforts of developing catalytic biofunctional membranes cantaining site - specifically immobilized enzyme was summarized involving biophysical chemistry and bioanalytical chemistry and chemical engineering, together with molecular biology - several kinds of novel immobilized technology were employed to effectuate the site - specific enzyme membrane immobilization
摘要介紹了從生物物理學、生物分析化學、化學工程學和分子生物學等學科角度研究開發的基於定點固定化技術的生物功能催化膜。Application of microdissection in molecular biology study of endometriosis
顯微切割取材方法在子宮內膜異位癥分子生物學研究中的應用After enzyme restriction and sequencing analysis, the nucleotide data had been further analyzed by antheprot 5. 0 and clutalw softwares. the analysis results showed that the cloned dna fragment had a longest open reading frame ( orf ) of 1035nt, it predicted to be encoded a 344 - aa protein with the molecular weight of 36kda
應用antheprot5 . 0 、 clustalw等分子生物學軟體分析,顯示主要外膜蛋白前24個氨基酸是較強的疏水性區域,可組成信號肽,其與omp基因的同源率達96 ,氨基酸的同源率高達98 。However, fc derivatives could not be used as functional molecules directly in many high - tech fields such as nonlinear optics, light emitting devices, sensors and so on. only when they are organized with some structure or dispersed homogeneously into solid matrix to form functional material, their function could be fully brought into play
在許多領域,如非線性光學、發光器件、傳感器等,二茂鐵衍生物並不能直接應用,但將具有光電活性的二茂鐵基團引入到兩親分子中,形成特定性能的成膜分子,就能真正發揮其作用。The investigation on the powerful laser induced micro - plasma by the radiation focused on a solid surface plays an important role in super - conduct films, nanometer materials, laser isotope separations, medicine, biology, and other industrial applications
對高功率密度激光輻照固體表面形成激光微等離子體的研究,在研製超導薄膜,納米材料,同位素分離,醫學,生物學,以及其他工業應用方面有重要的理論指導意義和廣泛的應用前景。分享友人