primary cell culture 中文意思是什麼

primary cell culture 解釋
原代細胞培養,原代細胞培養物
  • primary : adj 1 第一的，最初的，初級的；初等的；基本的；基層的。2 主要的，為首的，第一位的。3 原始的，根本...
  • cell : n 1 小室，單室；隔間，艙；〈詩〉茅舍；（單個的）蜂窩，蜂房。2 〈詩〉墓穴，墓。3 （大修道院附屬的...
  • culture : n. 1. 教養；修養；磨煉。2. 文化，（精神）文明。3. 人工培養，養殖；培養菌，培養組織。4. 耕作；栽培；造林。vt. 使有教養。
  1. The primary results showed : using m199 as diluents containing 20 % bovine serum, it is better to freeze the cells slowly freezing at fist then increase freezing speed ( for example, from 0 to - 6 freezing speed is about - 0. 05 a minute, from - 6 to - 40, freezing speed is about - 0. 5 a minute ), studies on effect of various concentration of dmso demonstrate that about 12. 5 % dmso gave the highest post - thaw percentage of viable cells. the concentration of bovine serum had no different effect on the percentage of the viable embryo cells of misgurnus auguillicaudatus. the embryo cells derived 6 from the later stage of blastula offish is more resistant to the cryogen than the cells of early stage of blastula. the cells preserved in liquid nitrogen at - 196 were thawed and cultivated, a few cells were found adhere to the surface of culture vessel when the percentage of viable cell was more than 30 %. the cells in only two culture vessels were found to proliferated and gave rise to many small morphologically undifferentiated cells

    研究初步表明:以細胞培養液m199 （含2既的小牛血清，常規量雙抗）為凍存稀釋液對泥鰍胚胎細胞冷凍保存宜採取先慢后快的方式（例如，從0一一6 ，凍存速度為一0 . 05 / min ，再以一0 . 5 / min的速度從一6一一40 ） ; dmso的保護效應濃度為12 . 506左右;小牛血清的濃度對泥鰍胚胎細胞的成活率影響不明顯;囊胚晚期細胞抗凍性比中早期強;通過對不同批次的凍存細胞解凍培養，解凍后成活率為30 %以上細胞培養數天後均有少數細胞貼壁，但只發現兩瓶培養細胞有明顯增殖現象產生許多未分化的小細胞。
  2. Part 1 : the culture and identification of es - d3 cells and the study of the efficiency of eb formation from es cells when grown on mef feeder layer in es culture medium or cultured in es culture medium supplemented with lif 1000u / ml, es - d3 cells being used in our experiments formed normal clones, expressed akp and kept their normal karyotype over many passages. the in vitro and in vivo differentiation experiments showed that es - d3 cells could differentiate into variety of cell types derived from three primary germ layers

    結果顯示： eso3細胞在小鼠胚胎成纖維細胞上和或含白血病抑制因於億f ）的es細胞培養液中形成典型的胚胎幹細胞克隆，堿性磷酸酶染色結果為強陽性，具有正常二倍體核型以及具有在體內外分化為三個胚層來源的組織細胞的潛能，而且具有形成種系嵌合動物的能力。
  3. For the cryogenic preservation of fish, in this paper we made the primary culture of the kidney of allotetroploid crucian carp and primary studies were carried out on cryopreservation culture offish embryo cells derived from misgurnus auguillicaudatus or grass carp, the results of the experiments are as follows : the primary cell culture of the kidney tissue derived from allotetroploid crucian carp was carried out using tissue adherent culture, the primary observations of the growth conditions and morphology of the primary culture and subculture cells which originally come from the kidney tissue were also made

    本文主要從魚類種質保存的目的出發，一方面以四倍體鯽鯉魚為材料，對四倍體鯽鯉魚腎臟組織進行初步培養，為建立相應細胞庫及下一步培養凍存的魚類胚胎細胞奠定基礎；另一方面，以魚類組織細胞培養技術為基礎，泥鰍胚胎細胞為材料，對魚類胚胎細胞凍存培養方法進行初步研究，並應用該技術方法對草魚早期胚胎細胞進行凍存培養實驗。報告如下：本文用組織貼壁法對四倍體鯽鯉魚腎組織進行原代、傳代培養。
  4. The results as follows : 1 ) the primary culture of qinchuan - scalper skin cells could be derived by fragment of tissue dispersed and cold digested single cell, collegenase i ( 150iu ml - 1 " ) and trypsin ( 0. 05 % ) being used at the same time is best to dissociate qinchuan - scalper skin tissue, which is appropriate to obtain qinchuan - scalper skin cells

    組織塊法、分散單細胞及dispase冷消化法單細胞均能獲得好的秦川牛皮膚組織原代培養物。其中150iu ? ml ~ （ - 1 ）膠原酶i與0 . 05胰蛋白酶（ 1 ： 1 ）同時應用能更好的分離秦川牛皮膚組織，是獲得秦川牛皮膚細胞的適宜方法。
  5. Primary culture of rat preadipocyte were prepared from the epididymal, inguinal and perirenal the fat pads of male normal, healthy, 15 - 20 days sprague - dawley rats. the preadipocyte grew better under the condition of 37, 95 % humidity, 5 % co2, ph 7. 0 - 7. 2, centrifuged at 1000r / min, m199medium, and 10 % fetal bo vine serum, seeded at a density of 4 l04, 5 l04, / cm2. oil red o staining was the special method to distinguish adipocyte from other cells, gimsa and he could determine the stage of the adiopcyte differentiation through the number of lipid drop, size and the position of the nucleolus of the staining fat cell

    經過多次實驗，確定本實驗室大鼠前體脂肪細胞的最佳培養條件是：溫度為37 ，濕度為95 ， co _ 2濃度為5 ， ph值為7 . 0 7 . 2 ，離心力為1000r / min ，培養基為m _ （ 199 ）培養基，胎牛血清濃度為10 ，合適細胞接種密度為4 10 ~ 4 、 5 10 ~ 4個/ cm ~ 2 ，染色結果表明：油紅o染色是鑒定脂肪細胞的特異方法， gimsa和he染色可根據不同區域染色程度、著色差別判斷細胞核的位置及脂滴大小、多少，觀察大鼠前體脂肪細胞分化過程中的形態變化，進而確定脂肪細胞的分化階段。
  6. During the course of the primary culture and passage of es cells, co - culture of mouse es cells and mef was the best. in this research, cell digestive juice with 0. 125 % trypsin + 0. 02 % edta was relative gentle to cells

    消化液濃度以0 . 125胰酶+ 0 . 02 edta對es細胞的損傷力最小，傳代后es克隆出現率最高。
  7. Prolonged the culture term, hepatocytes both of experimental groups and control groups became pyknotic and were detached from the wall or fibroblasts became prominent in the culture pl ate. it was important to note that none of the condition caused an increase in the number of cell in the whole experimental process. conclusion : the primary hepatocytes in medium of additional special nutritive may benefit in comparison with common medium

    結論：在培養液中添加轉鐵蛋白、牛胰島素、煙酚胺、 p琉基乙醇以及促肝細胞生長因子，可以促進原代小鼠肝細胞的貼壁生長，並且可以改善肝細胞在體外的代謝，使之維持良好的生活狀態並且延長體外生存時間。
  8. In 96 - well plates, effects of nutritional factors including glutamine, pyruvic acid, iron citrate, silicon, rpmi 1640 and marine broth 2216 were examined on the viable cell density in primary cell culture of s. domuncula after 36 hours of inoculation

    長期培養發現了adcp一p ~ orph中的骨針生長等現象，進一步證明了adcp一pri ~ orph中旺盛的代謝活動。通過兩種方式進行adcp一pri ~ orph傳代培養的初步探索。
  9. By this method of primary culture, cells and tissues could not only subsist for 11 months and more in vitro, but also be subcultured. these works lay a foundation of the establishment of silkworm embryonic cell line

    為建立新的家蠶胚胎細胞系奠定了一定的基礎。原代培養的細胞和組織塊在體外有一定的生長現象，細胞體外最長存活達門個月以上。
  10. There was about 7 days interval between every two passages. eg cell clones can be found in each primary culture from those embryos of appropriate age. in culture of one embryonic cell line, eg cell clones maintained after 9 passages

    在實驗中，幾個適齡的胚胎的原代培養物中都出現了鳥巢狀的eg細胞集落，維持時間最長的一個胚胎細胞株在傳代培養了9代之後仍然可以觀察到eg細胞集落。
  11. Cell adhesion to surface of the substrate is essential to development of the anchorage - dependent cells. only after adhering to surface followed by spreading can cells develop and proliferate. most synthetic polymers used as orthopaedic matrix substitute present hydrophobicity, which may correlates to the low degree of cell attachment. modification with cell adhesion protein / peptides can be benificial to the cell adhesion on polymers and then affect the cell proliferation and differentiation. cell attachment to substrate is primarily mediated by integrins, a widely expressed family of heterodimeric surface receptors. most extrcellular matrix proteins such as fibronectin, osteopontin, collagen type i, bone sialoprotein and vitronectin contain an arg - gly - asp ( rgd ) sequence which is specific to the fixation of cell membrane receptors like integrin. the main aim of this research is to measure, assess adhesion, proliferation of rabbit marrow stromal cells ( mscs ) on the polymers coated by fibronectin, collagen type i or biotie gen, which includes : ( 1 ) biologic characteristics of rabbit mscs were observed by two types of separating method in primary culture. ( 2 ) adhesion, proliferation and differentiation of mscs cultured on polymers coated with biotiegen were assessed. ( 3 ) also, adhesion, proliferation and differentiation of mscs were assessed on plga film or porous plga substrates coated with fibronectin, or collagen type i respectively. ( 4 ) bone formation was observed on the porous plga substrates coated with collagen type i in vivo. this research aims to give new way to make novel synthetic bone with cell adhesion and high bone induction capabilities

    因此將這些蛋白包被、固定到材料表面，觀察骨組織工程種子細胞mscs細胞的粘附、生長特性是本研究的中心環節，並從以下方面進行探討： （ 1 ）採用不同原代細胞分離方法，研究其對mscs細胞的生物學特性影響。 （ 2 ）檢測基因勝肽膠對mscs細胞粘附、增殖及分化的影響。 （ 3 ）分別採用型膠原及纖維粘連蛋白（ fibronectin ， fn ）包被聚乙醇酸-乳酸共聚物（ poly （ 1actide - co - glycolide ） ， plga ）膜及多孔塊型plga材料，觀察細胞在單層或三維培養狀態下，型膠原及fn對mscs細胞粘附、增殖及向成骨細胞分化效應及能力。
  12. Research, japan. there dr. jing helped to set up a primary culture system of neuroepithelial cells of day 10 mouse embryos. then he found a human teratocarcinoma cell line, pa - 1, secreted the growth factors related to egf and igf - i

    曾於1989 1991年赴日本理化學研究所進行博士后研究；於1992 1993年以訪問學者身份赴日本理化學研究所進行合作研究； 1995年以訪問學者身份在法國strasbourg的遺傳與分子細胞生物學研究所（ gbmc ）進行合
  13. The two mediums can meet primary culture and passage culture of the black bear fibroblast cells. the method of single cell cloning by micro - manipulating purifies fibroblast cells. as a result, steady fibroblast cells can be obtained and the rates of the cloning formation is 93. 75 %

    通過單細胞集落顯微法純化細胞，可克服單細胞克隆中因細胞密度太低，難以形成克隆的缺點，而且操作簡單，效率高，速度快，其克隆率可達93 . 75 。
  14. The development of a growth medium is one of the essential prerequisites for sponge cells grown in vitro. the mtt ( 3 - ( 4, 5 - dimethylthiazol - 2 - yl ) - 2, 5 - diphenyltetrazolium bromide ) assay was adapted to screen potential nutritional factors in formulating a growth medium for a primary cell culture of suberites domuncula

    Adcp一pri ~ orph培養具有與mcp一pri ~ orph顯著不同的成團過程，增殖細胞比例提高了近3倍，細胞最大生長達到接種時的4倍，明顯優于mcp一pri ~ orph培養。
  15. Research on the ratio method of measuring intracellular ca2 + concentration with ratio fluorescence imagemaster was reported in the last chapters, which include the folio wings : ( 1 ) the ratio experiment system of measuring intracellular ca concentration with ratio fluorescence imagemaster was established ; ( 2 ) the primary culture mode of suckling mouse cardiac muscle cell was established and the ca2 + concentration in suckling mouse cardiac muscle cell was measured. after adding ne ( norepinephrine ) to cardiac muscle cell, the relative fluorescence intensity was dramatic increased

    本文後半部分主要圍繞用比率熒光光譜儀測量細胞內游離ca ~ （ 2 + ）的研究進行了以下工作： （ 1 ）建立了比率熒光光譜儀測量細胞內游離鈣離子濃度的實驗系統； （ 2 ）建立了乳鼠心肌細胞原代培養的模型，並對培養細胞進行了細胞內ca ~ （ 2 + ）濃度的測量。
  16. However, at 3 ~ 4 days primary culture, almost all the pgcs were formed of 2 ~ 20 cell mass. after 5 ~ 7 days, the numbers of putative eg clonies increased distinctly

    培養3 4天的pgcs大多已轉變為由2 20細胞構成的集落，其中包括從pgcs群集或細胞本身增殖所形成的兩種集落類型。
  17. This dissertaion, on the basis of the other studies, on the one hand, researched and analysed the chromosomes and mitoses of the cell line - bmn, which is widely used in the silkworm baculovirus expression system as an engineering cell. on the other hand, the dissertation attempted to explore establishment of the new silkworm embryoni cell line by primary culture

    同時，家蠶體外細胞培養研究的進行，不僅可以為家蠶細胞生物學的基礎理論研究提供良好的研究系統，而且在家蠶資源的開發與利用方面也具有重大的意義。本研究在借鑒前人研究的基礎上，一方面對現在廣泛應用於家蠶桿狀病毒表達系統的工程細胞? bmn細胞的染色體和有絲分裂進行了研究與分析。
  18. Using misgurnus auguillicaudatus as material, we made a primary study of embryonic cell preparation, cryopreservation, thawing and primary cell culture.,

    另一個方面的有關原理技術，本文主要以泥鰍胚胎細胞為材料，根據細胞凍存，進行泥鰍胚胎細胞制備、冷凍保存、解凍、原代培養研究。
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