endoglucanase 中文意思是什麼

endoglucanase 解釋
內葡聚醣酶
  1. Sequencing analysis showed that the sequence of endoglucanase fragment exhibits 35 % homology with b. subtil is chromosomal dna ( from glyb to apre ), and 27 % homology with bacillus sp

    將此重組質粒phchi轉化巨大芽抱桿菌b . megateriumap25 ，得到兩株轉化子，分別命名為p25113一9 ， p25113一10 。
  2. By transformation with the genes. plant disease biocontrol bacteria bacillus subtil is aplls and b. megaterium ap25 were isolated from wheat field soils collected from south australia and tai an. enzyme activity analysis on chitin agar and abp media showed that b. subtilis aplls secreted chitinase and b. megaterium ap25 secreted endoglucanase, respectively

    測序后在genebank上進行序列比較，該基因片段同編號為2634966的枯草芽孢桿菌全序列的2599451到2812870 （功能未知）有85的同源性，但同已發表的13種幾丁質酶的基因（包括枯草芽孢桿菌幾丁質酶基因）的同源性很低，只有30 。
  3. Its km for sacilin at50 andph5. owas3. 73mg / ml. with the analysis of kinetics, the p - glucosidase showed synergistic action to the endoglucanase and the endoglucanase inhibited the p - glucosidase. the mechanism for their interaction was explained that the endoglucanase was combined with its products and the inhibition of these products to endoglucanase was removed by its hydrolysis by p - glucosidase

    經兩組分共同作用的酶反應動力學分析后發現，兩組分這種相互作用機制的核心是：內切酶和?葡萄糖苷酶與內切酶的水解產物，也是其底物類似物? ?纖維二糖和纖維寡糖的結合以及-葡萄糖苷酶對該產物的水解。
  4. Bp23 celb genes, b. pwnilus endoglucanase and b. polymyxa beta - 1, 4 - endoglucanase " genes, respectively. it was recognized as a new gene encoding for endoglucanase of b. mega terium. the recombinant plasmid tvchi ( pmd18 ~ t inserted with chitinase encoding gene from aplls ) and e. coli - bacillus shuttle vector physooplk were digested by ecori and sail completely, and the chitinase gene was ligated with shuttle vector, and the recombinant vector was used to transform b. megaterium ap25 competent cell

    平板拮抗實驗同野生菌株相比，轉化子對麥長蠕抱菌的抑制作用最明顯，抑制百分數最高可達33 . 3 % ，而apll3和ap25分別是23 . 1 %和25 . 6 % ，同時轉化子對小麥紋枯病菌、棉花立枯病菌、棉花枯萎病菌和小麥的全蝕病菌也具有較為明顯的抑制作用。
  5. 3. 2. 1. 4 ) which was prepared by precipitation of the water extract of the culture of aspergillus niger with ammonium sulfate and desalted by sephadex g - 25, and was further fractionated by two steps of deae - toyopearl 650m and one step of poros 20pi chromatography. the other was a p - glucosidase ( ec. 3. 2. 1. 21 ) which was prepared by the above g - 25 fractions and was further fractionated by two steps of deae - toyopearl 650m chromatography. the specific activity of the endoglucanase with sodium carboxymethyl cellulose was estimated to be 433. 38 hj / mg

    -葡萄糖苷酶對水楊素的比活力為597 . 12iu mg ，並對其專一，不能水解棉花和羧甲基纖維素鈉；分子量為117 . 5kda ，加dtt後分子量不變；該組分最適ph和溫度分別為4 . 5和70 ，在ph5 . 0 、 50下對水楊素鈉的米氏常數km為3 . 73mg ml ，最大反應速度vm為0 . 088mg葡萄糖（ ml ? min ） ；與文獻中從黑麴黴中分離的-葡萄糖苷酶比較后發現，該組分是一個新的-葡萄糖苷酶。
  6. This article dealt with cloning and sequencing of chitinase and endoglucanase genes of bacillus spp. and recombinant biocontrol isolates of bacillus spp

    質粒分析證明克隆子中含有重組質粒，外源插入片段大小為2 . 6kb左右，與pcr最初產物的大小一致。
  7. It was thought to be a new gene encoding for chitinanse of b. subtilis. genome library methodology was adopted for cloning of endoglucanase encoding genes

    雖然同源性較低，但酶活性表達較強，認為該基因是編碼內切葡聚糖酶的一個新基因片段。
  8. Genoraic dna of b. megaterium was partially digested by restriction enzyme sau3a and was used to establish the genomic library in plasmid pbluscripts. selection for endoglucanase positive clones from transformed e. coli was carried out on the cmc medium. seventy - four clones that showed hydrolysis ability on the cmc plate were obtained

    用ecori和sall對含有幾丁質酶基因的重組質粒tvchi （含幾丁質酶編碼基因的pmd18一t載體）和穿梭質粒phy300plk進行雙酶切並連接，轉化e . colidhs 。
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